Magnetic cell sorting and flow cytometry sorting methods for the isolation and function analysis of mouse CD4＋ CD25＋ Treg cells

Objective: In this paper we compared the two methods of cell sorting (magnetic cell sorting and flow cytometry sorting) for the isolation and function analysis of mouse CD4~+ CD25~+ regulatory T (Treg) cells, in order to inform further studies in Treg cell function. Methods: We separately used magnetic cell sorting and flow cytometry sorting to identify CD4~+ CD25~+ Treg cells. After magnetic cell separation, we further used flow cytometry to analyze the purity of CD4~+ CD25~+ Treg cells, trypan blue staining to detect cell viability, and propidium iodide (PI) staining to assess the cell viability. We detected the immune inhibition of CD4~+ CD25~+ Treg cells in the in vitro proliferation experiments. Results: The results showed that compared to flow cytometry sorting, magnetic cell sorting took more time and effort, but fewer live cells were obtained than with flow cytometry sorting. The CD4~+ CD25~+ Treg cells, however, obtained with both methods have similar immunosuppressive capacities. Conclusion: The result suggests that both methods can be used in isolating CD4~+ CD25~+ Treg cells, and one can select the best method according to specific needs and availability of the methodologies.     

下乡家电运筹式TOC供应链系统订单排序模型研究

从下乡家电中标企业供应链系统的全局出发,结合数学与TOC方法,分析了多订单条件下家电产品供应的排序问题,提出了基于TOC的供应链系统中的订单排序法,给出了运筹式TOC供应链系统中的订单排序模型,提供了基于TOC供应链系统的运筹管理示例.该方法可以有效地降低供应链系统中的库存,提高家电下乡服务的水平,增加企业有效产出.     

零散有存档权PDF文件的采、编、检管理工具

通过"电子文献网络结构技术",来解决国内重点关注的"对开放存取数字全文资源即采、即编、即检管理系统"问题。实现了本地允许保存零散PDF文件的再标引,通过云计算技术实现了对文献题录的时时采集、时时编辑、时时检索。通过PubMed中PMID编号自动链接PDF,实现允许保存的零散PDF电子资源即采、即编、即检管理系统。     

基于果蝇优化算法的模拟滤波器设计

基于粒子群优化算法的无源模拟滤波器优化设计方法容易陷入局部最优,收敛速度慢迭代次数多、运算量大且稳定性不够好。提出果蝇优化算法对滤波器的整个参数空间进行高效并行搜索直到获得最优的参数值,实例仿真表明,采用该方法设计的滤波器在相同的带宽准确度及阻带衰减的情况下,具有更快的运算速度及收敛性能。     

Organizing Interpretive Text for a Digital Library: How User Testing Found the Lost Frontier

SUMMARY

This paper looks at exercises done with user focus groups and how they have influenced the reorganization of Meeting of Frontiers' interpretive text content. Meeting of Frontiers is an international collaborative digital library project at the Library of Congress. Testing consisted of a user survey and card sorting exercises in order to refine the site hierarchy and identify problems with design proposals.     

Card-Sorting Usability Tests of the WMU Libraries’ Web Site

ABSTRACT

This article describes the card-sorting techniques used by several academic libraries, reports and discusses the results of card-sorting usability tests of the Western Michigan University Libraries’ Web site, and reveals how the WMU libraries incorporated the findings into a new Web site redesign, setting the design direction early on. The article briefly describes open and closed card-sorting techniques and quantitative and qualitative methods of analyzing data commonly used in computer and library science fields. Findings from this study allowed the library design team to vastly improve its initial redesign decisions for a new Web site tabbed navigation system. Card sorting not only helped the design team validate its redesign decisions, but it also opened the WMU libraries to outside innovation, inviting Web site visitors to redesign the Web site free from the libraries’ influence. The simple and inexpensive techniques used here may be useful to any Web librarian or design team embarking upon redesign and usability testing of their own sites and interested in building a more compelling, insightful Web site.     

Toward a Usable Academic Library Web Site: A Case Study of Tried and Tested Usability Practices

Usability testing is an important element when designing useful, usable academic library Web sites. Since 2001, members of the Florida International University Libraries Web team have worked toward establishing a process that identifies user needs through usability testing. Starting with the libraries’ first Web site redesign project in 2001, the team has taken an active part in improving Web site user experience. After engaging in multiple redesign efforts, the Web team has developed a process that supports awareness of user experience through continual usability testing and feedback gathering. This article presents strategies and practices to measure Web site user experience, including classic usability testing methods (e.g., card sorting activities, focus groups, and task-based user testing), as well as the team's latest effort to assess Web site analytics and content to identify Web site usage patterns and areas of concern. A history of the Florida International University Libraries’ Web site redesign process is presented to illustrate lessons learned and best practices to facilitate future redesign and testing efforts. Taking a longitudinal look at usability testing at one institution, the study aims to inform the development of an effective strategy for user research and content management.     

基于模板法的苹果图像与背景快速分离技术的研究

机器视觉对水果的品质进行检测并实现自动分级时,将水果图像从背芾中分割出来是一项非常重要的预处理步骤。其分割效果的好坏直接关系到后续分级的准确性。现提出一种基于模板法的图像与背蒂分离方法,通过大量的实验证明该方法对苹果与背景的分离是快速且有效的。     

表/数据库文件中名次数据的自动添加

本文针对FOX系列中用SORT命令不能给表 /数据库文件自动地填上名次字段这一问题 ,较为巧妙地使用两种实现方法解决了这一问题 ,具有较强的实践性和可参考性     

中国荞麦属果实形态特征

应用解剖镜和扫描电镜对中国产荞麦属Fagopyrum Mill．8种1变种的果实形态和微形态特征进行了观察。结果表明这些种类的果实可分为3类：(1)果实三棱锥状,表面不光滑,无光泽,具皱纹网状纹饰,此种类型的植物有苦荞麦、金荞麦。(2)果实卵圆三棱锥状,表面光滑,有光泽,具条纹纹饰,此种类型的植物有荞麦、长柄野荞麦、线叶野荞麦。(3)果实卵圆三棱锥状,表面光滑,有光泽,具大量的瘤状颗粒和少数模糊的细条纹纹饰,此种类型的植物有硬枝野荞麦、细柄野荞麦、小野荞麦、疏穗小野荞麦。研究结果认为金荞麦与栽培种苦荞麦关系较近。