生态学专业《3S技术及其应用》课程的教学实践与思考

根据"3S"技术在生态学领域中的应用和发展趋势,结合近年来华南农业大学生态学专业《3S技术及其应用》课程的教学实践,分析了该课程教学中存在的问题,针对性地选择了教学重点内容,探讨了该课程的实践教学方式,提出了该课程今后在实践教学方面的发展方向和教学改革建议,旨在不断提高该课程的教学质量。     

VDR,RXR, Coronin-1 and Interferonγ Levels in PBMCs of Type-2 Diabetes Patients: Molecular Link between Diabetes and Tuberculosis

Diabetes and tuberculosis are world’s most deadly epidemics. People suffering from diabetes are susceptible to tuberculosis. Molecular link between the two is largely unknown. It is known that Vitamin A receptor (RXR) heterodimerizes with Vitamin D receptor (VDR) and Peroxisome proliferator-activator receptor-γ (PPARγ) to regulate Tryptophan-aspartate containing coat protein (TACO) expression and fatty acid metabolism respectively, so it would be interesting to check the expression of these genes in diabetes mellitus (DM) patients which might explain the susceptibility of diabetics to tuberculosis. In this study, we checked the expression of RXR, VDR, TACO and Interferon-γ (IFNγ) genes in type-2 DM patients for understanding the link between the two diseases. We observed down regulation of RXR gene and corresponding up regulation of TACO gene expression. We have not observed significant change in expression of VDR and IFNγ genes in type-2 DM patients. Repression of RXR gene could hamper VDR-RXR heterodimer formation and thus would up regulate TACO gene expression which may predispose the type-2 DM patients to tuberculosis. Also, decrease in RXR-PPARγ heterodimer could be involved in DM.     

H3C网络实验室远程开放的设计与实现

为实现H3C网络实验室的远程开放,提高网络实验室的利用率,文章首先就网络实验室及实验机架的网络设计、网络设备集中管理以及SSL VPN技术特点等问题进行了阐述,然后结合实例给出了具体的配置实现方法。     

湘南土话知三、章读如端组的性质

湘南土话普遍存在知三、章读如端组的现象。读如端组时,韵母有的为洪音,有的为细音。考察知三、章读如端组时韵母的洪细及其与其他声纽的分合关系,发现,韵母为细音时,知三读如端组一般是"古无舌上音"的表现,而韵母为洪音时,读如端组的性质多种多样;章组读为端组则是与精、庄、知二或知三、见合流后的演变,属于晚期的演变。     

茶树中咖啡酰辅酶A氧甲基转移酶的基因克隆及其催化活性研究(英文)

目的:从茶树中克隆一种可以催化表没食子儿茶素没食子酸酯(EGCG)生成甲基化EGCG的酶——咖啡酰辅酶A氧甲基转移酶(CCo AOMT),实现甲基化EGCG的酶学合成,为甲基化EGCG的进一步开发利用提供理论依据和技术指导。创新点:本研究首次从茶树中克隆了一条CCo AOMT基因组序列;分析了CCo AOMT基因在不同茶树品种和不同成熟度茶鲜叶中的基因表达规律;证明了CCo AOMT具有催化合成甲基化EGCG的生物活性。方法:采用聚合酶链式反应(PCR)和序列分析获得CCo AOMT的编码序列和基因组序列;采用高效液相色谱-四级杆-飞行时间串联质谱技术(HPLC-QTOF-MS)分析酶促反应生成的甲基化EGCG产物(图4);采用实时荧光定量PCR分析CCo AOMT基因的表达差异(图5)。结论:本研究从茶树中克隆了CCo AOMT基因的编码序列(738 bp)和基因组序列(2678 bp),明确了该基因具有4个内含子和5个外显子;揭示了CCo AOMT可以催化EGCG生成EGCG4"Me、EGCG3"Me和EGCG3’Me等多种甲基化产物;证明了CCo AOMT具有催化生成甲基化EGCG的活性;并发现该基因的表达量高低与茶鲜叶的成熟度呈正相关关系。     

“3+4”衔接培养高层次技术技能人才的探究--以金陵科技学院土木工程专业为例

探索中职与应用型本科的有效衔接机制是江苏省现代职业教育体系建设试点项目的主要目的。分析了“3+4”衔接培养土木工程高层次技术技能人才的必要性、可行性。以金陵科技学院土木工程专业“3+4”项目为例,从项目的组织领导、分段培养目标的确定、课程体系的构建、各种能力的培养、教学组织与质量管理、学生教育管理等方面入手,提出分段衔接培养的策略和衔接机制。     

用友T3软件操作常见问题及对策

用友T3软件是会计信息化课程的常用教学软件之一。经过长期教学实践发现学生在操作中经常会出现账套号用错、演示版到期、凭证遗漏等问题,针对这些问题进行分析并探讨出通过数据库、账套输出文件及软件操作技巧等方式来有效解决的对策,供授课教师和软件学习者参考。     

我国3G发展现状与前景分析

今年是工业和信息化部为三大电信运营商发放第三代牌照的第二年,一年半以来我国3G终端、网络覆盖等方面取得了较大程度的发展,就我国3G发展状况及其发展前景等进行了分析。     

三维动画模型制作的混合建模流程探讨

要制作出一个优秀的三维动画模型需要具备艺术修养和软件技术,同时不能忽略制作方法及工作流程的总结和提炼。本文分析了各种制作方法,初步提出混合建模的工作流程,以探索三维动画模型的制作方法。     

A recessive gene controlling male sterility sensitive to short daylength/low temperature in wheat (Triticum aestivum L.)

Utilization of a two-line breeding system via photoperiod-thermo sensitive male sterility has a great potential for hybrid production in wheat (Triticum aestivum L.). 337S is a novel wheat male sterile line sensitive to both short daylength/low temperature and long daylength/high temperature. Five F₂ populations derived from the crosses between 337S and five common wheat varieties were developed for genetic analysis. All F₁’s were highly fertile while segregation occurred in the F₂ populations with a ratio of 3 fertile:1 sterile under short daylength/low temperature. It is shown that male sterility in 337S was controlled by a single recessive gene, temporarily designated as wptms3. Bulked segregant analysis (BSA) coupled with simple sequence repeat (SSR) markers was applied to map the sterile gene using one mapping population. The wptms3 gene was mapped to chromosome arm 1BS and flanked by Xgwm413 and Xgwm182 at a genetic distance of 3.2 and 23.5 cM, respectively. The accuracy and efficiency of marker-assisted selection were evaluated and proved essential for identifying homozygous recessive male sterile genotypes of the wptms3 gene in F₂ generation.