MET对油菜幼苗活性氧代谢的影响

试验采用质量比值分别为100×10(-6),150×10~(-6),200×10~(-6)的MET喷施二叶一心期油菜幼苗,于第7,14,21(或23)和45d随机取功能叶,以比色法测定POD活性,滴定法测CAT活性及GSH含量,抗坏血酸过氧化物酶活性采用紫外吸收法测定,结果表明,喷施多效唑的油菜幼苗叶中POD和抗坏血酸过氧化物酶活性增高,CAT活性降低,GSA含量增加,从而说明了MET提高作物的抗逆性,可能是由于提高某些保护酶活性及增加非酶类自由基净化剂含量所致。     

Differential generation of hydrogen peroxide upon exposure to zinc and cadmium in the hyperaccumulating plant specie （Sedum alfredii Hance）

Sedum alfredii Hance has been identified as zinc (Zn) and cadmium (Cd) co-hyperaccumulator. In this paper the relationships of Zn or Cd hyperaccumulation to the generation and the role of H2O2 in Sedum alfredii H. were examined, The results show that Zn and Cd contents in the shoots of Sedum alfredii H. treated with 1000 μmol/L Zn2 and/or 200 μmol/L Cd2 increased linearly within 15 d. Contents of total S, glutathione (GSH) and H2O2 in shoots also increased within 15 d, and then decreased. Total S and GSH contents in shoots were higher under Cd2 treatment than under Zn2 treatment. However, reverse trends of H2O2 content in shoots were obtained, in which much higher H2O2 content was observed in Zn2 -treated shoots than in Cd2 -treated shoots. Similarly, the microscopic imaging of H2O2 accumulation in leaves using H2O2 probe technique showed that much higher H2O2 accumulation was observed in the Zn2 -treated leaf than in the Cd2 -treated one. These results suggest that there are different responses in the generation of H2O2 upon exposure to Zn2 and Cd2 for the hyperaccumulator Sedum alfredii H. And this is the first report that the generation of H2O2 may play an important role in Zn hyperaccumulation in the leaves. Oar results also imply that GSH may play an important role in the detoxification of dissociated Zn/Cd and the generation of H2O2.     

金樱子果肉Cu·Zn—SOD纯化及其活性测定

通过（ＮＨ４）２ＳＯ４分级沉淀和ＳｅｐｈａｄｅｘＧ—１００分离提纯得到金樱子Ｃｕ·Ｚｎ—ＳＯＤ，经聚丙烯酞胺凝胶电泳发现有５—６条同工酶谱带，最明显的３条迁移率分别为０．４３，０．４６和０．５５，ＳＤＳ—ＰＡＧＥ测得亚基分子量约为１３ＫＤ，其活性被２ｍｍｏｌ／ＬＫＣＮ或Ｈ２Ｏ２抑制，不受氯仿—乙醇影响，在ｐＨ７．６磷酸缓冲液中活性最大，对热相对稳定。ＳＯＤ活性与ＶＣ、ＧＳＨ含量有明显的相关性，相关系数为０．９３４２和０．９１０６，果实由金黄转褐黄阶段有—ＳＯＤ活性高峰。认为以Ｖｃ为指标可以判断ＳＯＤ活性水平。     

限制肌血流量运动训练对非系统训练者血中抗氧化酶活性影响的实验研究

通过研究递增缺血强度的限制肌血流量运动训练前后血中抗氧化酶活性的变化 ,探讨限制肌血流量运动训练对机体脂质过氧化水平和抗氧化能力的影响。     

中等强度运动对去卵巢大鼠子宫刺激、骨骼肌MDA含量及SOD、GSH-PX活性的影响

目的:探讨中等强度运动对去卵巢子宫刺激、骨骼肌MDA含量及SOD、GSH-PX活性的影响。方法:将48只3月龄雌性大鼠按体重随机分成假手术组(Sham组)、造模组(Model组)、己烯雌酚组(DES组)、运动组(EX组),每组12只,假手术组仅切除卵巢附近一团脂肪,其余各组均切除双侧卵巢。术后按不同要求灌胃给药和运动,12周后称重后处死大鼠。分离子宫,测量子宫湿重及子宫内膜厚度。取各组大鼠股四头肌制备成10%的组织匀浆行MDA、SOD、GSH-PX检测,统计分析各组数据。结果:与DES组比较,其它各组体重显著降低(P<0.05或P<0.01);与DES组比较,其它各组子宫内膜厚度极显著降低(P<0.01);与Model组比较,DES组和EX组MDA均显著升高(P<0.05),SOD、GSH-PX均显著下降(P<0.05或P<0.01)。结论:中等强度的运动能维持去卵巢大鼠体重;对子宫刺激作用较雌激素低;能显著降低去卵巢大鼠骨骼肌MDA含量,增强SOD、GSH-PX活性,减少自由基的堆积,提高机体抗氧化能力,改善骨骼肌性能,延缓骨骼肌衰老。较雌激素药物干预更安全,优势明显,可作为临床选择。     

从酵母细胞中抽提谷胱甘肽的研究初探

还原型谷胱甘酞(L-GSH)是一种天然的强还原性短肽,具有清除体内自由基,解毒护肝等生理功能.考察了有机溶剂、pH、温度和菌体浓度等不同条件从酵母湿菌体中提取谷脱甘肽(GSH)的抽提效果.比较分析结果表明.8%-10%浓度乙酸乙酯在pH值60左右抽提效果最好,其抽提收率达90%以上、耗时短、经济性强而明显.     

外源GSH对盐胁迫下大麦幼苗生长的影响

３００ ｍｍｏｌ／Ｌ ＮａＣｌ明显抑制大麦幼苗的生长, ＮａＣｌ处理的同时加入 ２０ ｍｇ／Ｌ的 ＧＳＨ可明显增加大麦幼苗干重、根系长度、叶片叶绿素以及Ｋ＋含量,降低Ｎａ＋含量;同时叶片液泡膜Ｈ＋－ＡＴＰａｓｅ和Ｈ＋－ＰＰａｓｅ的活性均明显增加,电解质渗漏率降低．     

Oxidant-antioxidant status in colorectal cancer patients—Before and after treatment

Erythrocyte antioxidant glutathione and malondialdehyde levels in erythrocytes and plasma glutathione S-transferase levels were estimated in patients with colorectal cancer and compared to controls. Further, the patients underwent four weeks of radiotherapy with adjuvant chemotherapy. The same parameters were estimated after four weeks of radiotherapy and compared with pretreatment levels. It was observed that there was a decrease in erythrocyte glutathione and malondialdehyde levels in patients with colorectal cancer compared to controls, but not in case of GST. However, after chemoradiotherapy, there were no statistically significant differences in all the parameters studied.     

低氧运动对血清MDA、SOD、GSH的影响及电解质饮料干预效果的研究

探讨了低氧运动对血清MDA、SOD、GSH的影响及补充电解质饮料的干预效果.8名受试者每周在15.4%低氧环境下以70%VO2max强度蹬功率自行车1 h,常氧下恢复,共持续3周,并分别在运动前0.5 h、运动后即刻、运动后0.5 h及运动后1 h采静脉血,测定血清MDA、SOD、GSH.第一周运动期间不补充任何液体,以研究低氧运动对血清MDA、SOD、GSH的影响.第二周运动期间补充不含抗氧化剂的电解质饮料--宝矿力水特,第三周作为对照补充纯净水,以研究电解质饮料干预效果.结果表明低氧运动后血清MDA没有产生明显变化,但血清SOD、GSH显著性提高(P＜0.05),可能是机体加快自由基清除的代偿性反应;低氧运动中补充电解质饮料或纯净水,运动后SOD、GSH均不发生显著性改变,说明低氧运动时补充水分有助于减少自由基的产生.     

"复方抗氧化制剂"对训练大鼠一次运动后骨骼肌谷胱甘肽抗氧化系统影响的研究

为探讨"复方抗氧化制剂"及运动训练对大鼠骨骼肌谷胱甘肽抗氧化系统的影响,给训练大鼠补充"复方抗氧化制剂",结果显示:训练组、补充组大鼠在安静状态骨骼肌中GSH-PX活性、GSH含量都明显高于对照组(P<0.05),GST活性与对照组相比变化不明显,在定量负荷运动后,GSH含量都明显高于对照组(P<0.05),在力竭运动后GSH含量、GST活性都明显高于对照组(P<0.05).提示:"复方抗氧化制剂"能够并协同运动训练增强大鼠骨骼肌谷胱甘肽抗氧化系统能力,提高机体抗氧化能力.