康脑液对皮质区干细胞因子基因表达的影响

目的:在康脑液方剂干预下观察皮质区内源性神经细胞干细胞因子(SCF)mRNA在大鼠脑缺血再灌注损伤后不同时间的表达情况。方法:成年SD大鼠,以线栓法建立大脑中动脉缺血再灌注模型,随机分为模型组、药物干预组和假手术组。原位杂交技术检测脑缺血1.5h再灌注1～14d后,脑皮质区SCFmRNA表达情况。结果:脑缺血再灌注后,药物干预组、模型组SCFmRNA的表达在皮质区均明显高于假手术组,于第7天达高峰,第14天下降。结论:药物干预后脑缺血再灌注脑皮质区SCFmRNA表达在不同时间点与模型组具备相同的表达规律,两组SCFmRNA的表达无显著性差异。     

CRISPR/Cas: a Nobel Prize award-winning precise genome editing technology for gene therapy and crop improvement

Since it was first recognized in bacteria and archaea as a mechanism for innate viral immunity in the early 2010 s,clustered regularly interspaced short palindromic repeats(CRISPR)/CRISPR-associated protein(Cas) has rapidly been developed into a robust, multifunctional genome editing tool with many uses. Following the discovery of the initial CRISPR/Cas-based system, the technology has been advanced to facilitate a multitude of different functions. These include development as a base editor, prime editor, epigenetic editor, and CRISPR interference(CRISPRi) and CRISPR activator(CRISPRa) gene regulators. It can also be used for chromatin and RNA targeting and imaging. Its applications have proved revolutionary across numerous biological fields, especially in biomedical and agricultural improvement. As a diagnostic tool, CRISPR has been developed to aid the detection and screening of both human and plant diseases, and has even been applied during the current coronavirus disease 2019(COVID-19) pandemic. CRISPR/Cas is also being trialed as a new form of gene therapy for treating various human diseases, including cancers, and has aided drug development. In terms of agricultural breeding, precise targeting of biological pathways via CRISPR/Cas has been key to regulating molecular biosynthesis and allowing modification of proteins, starch, oil, and other functional components for crop improvement. Adding to this, CRISPR/Cas has been shown capable of significantly enhancing both plant tolerance to environmental stresses and overall crop yield via the targeting of various agronomically important gene regulators. Looking to the future, increasing the efficiency and precision of CRISPR/Cas delivery systems and limiting off-target activity are two major challenges for wider application of the technology. This review provides an in-depth overview of current CRISPR development, including the advantages and disadvantages of the technology,recent applications, and future considerations.     

真核生物基因表达的转录后调控

本文概要叙述了在核mRNA加工成熟过程中各种调控基因表达的方式。     

瞄准定位 变中求新——从《小学教学设计》的持续发展看教学类期刊的编辑创新

近年来以教师为读者对象的教学类期刊订数普遍滑坡,而<小学教学设计>2008年的邮局订数却再次跃上新高,成为同类刊物的佼佼者.<小学教学设计>之所以取得这样的成绩,主要在于有明确的编辑思想,面对变化的期刊市场,面对动态中的小学教学改革,能始终瞄准定位,以变应变,变中出新,以新取胜.     

植物抗虫基因工程的研究

在当今的农业生产中,虫害问题已成为减产的重要因素之一,随着植物基因工程的兴起与发展,该问题已在很大程度上得到解决。本文介绍了利用基因工程手段而分离到的几种抗虫基因,为抗虫基因工程的发展奠定了基础,并探讨了植物抗虫基因工程中存在问题和解决方案。     

PCR技术在肿瘤检测及治疗中的应用

本文概述了聚合酶链反应技术在癌基因、抑癌基因、端粒酶的活性、肿瘤病毒、肿瘤免疫的研究及常见肿瘤的检测及预后等方面的应用。     

Effects of medium composition on the production of plasmid DNA vector potentially for human gene therapy

INTRODUCTION The difficulties associated with large-scaleproduction of biotherapeutics provide a constantchallenge to the biotechnology industry. FDA hadadded “therapeutic DNA plasmid vectors” to the listof well-characterized biotechnology product (DoHHs,1996), and gene therapy has moved rapidly fromlaboratory scale to clinical trials. It is urgent to de-velop new protocols to obtain high-quality plasmidswith high yields and minimal or no contamination ofRNA and chromosomal D…     

基因沉默及其生理学意义

基因沉默包括转录水平上的基因沉默和转录后水平上的基因沉默。发生沉默的基因可以是外源性转移基因,也可以是入侵的病毒或宿主内源性基因。双链RNA(dsRNA)作为启动因素或中间体为不同生物基因沉默所共有。基因沉默是生物体在基因调控水平上的一种自我保护机制。通过基因沉默,可以限制外来核酸的入侵;同时,基因沉默还与生物的生长发育有关。     

生物芯片及应用

本概述了生物芯片的发展历史、制作方法及其广阔的应用前景，分析了基因芯片领域的最新成果。生物芯片的发展，必将对人类生活产生深远的影响。因此，生物芯片的研究受到人们的广泛关注。