全文获取类型
收费全文 | 398篇 |
免费 | 1篇 |
国内免费 | 16篇 |
专业分类
教育 | 262篇 |
科学研究 | 123篇 |
体育 | 6篇 |
综合类 | 12篇 |
信息传播 | 12篇 |
出版年
2022年 | 1篇 |
2021年 | 8篇 |
2020年 | 3篇 |
2019年 | 5篇 |
2018年 | 2篇 |
2017年 | 6篇 |
2016年 | 4篇 |
2015年 | 11篇 |
2014年 | 22篇 |
2013年 | 24篇 |
2012年 | 19篇 |
2011年 | 27篇 |
2010年 | 22篇 |
2009年 | 42篇 |
2008年 | 30篇 |
2007年 | 43篇 |
2006年 | 30篇 |
2005年 | 30篇 |
2004年 | 21篇 |
2003年 | 12篇 |
2002年 | 19篇 |
2001年 | 4篇 |
2000年 | 7篇 |
1999年 | 1篇 |
1998年 | 6篇 |
1997年 | 4篇 |
1996年 | 1篇 |
1995年 | 1篇 |
1994年 | 3篇 |
1993年 | 3篇 |
1992年 | 2篇 |
1991年 | 2篇 |
排序方式: 共有415条查询结果,搜索用时 31 毫秒
71.
本文提出了一种求解带约束非线性规划问题的一般方法。与现有的方法相比 ,它不受可导性 ,单峰性和是否病态等因素的限制 ,适用范围广。实例说明该算法对带约束非线规划问题的求解十分有效 相似文献
72.
WU Xiao-juan ZHU Xin-jian CAO Guang-yi TU Heng-yong 《浙江大学学报(A卷英文版)》2007,8(9):1505-1509
The solid oxide fuel cell (SOFC) is a nonlinear system that is hard to model by conventional methods. So far,most existing models are based on conversion laws,which are too complicated to be applied to design a control system. To facilitate a valid control strategy design,this paper tries to avoid the internal complexities and presents a modelling study of SOFC per-formance by using a radial basis function (RBF) neural network based on a genetic algorithm (GA). During the process of mod-elling,the GA aims to optimize the parameters of RBF neural networks and the optimum values are regarded as the initial values of the RBF neural network parameters. The validity and accuracy of modelling are tested by simulations,whose results reveal that it is feasible to establish the model of SOFC stack by using RBF neural networks identification based on the GA. Furthermore,it is possible to design an online controller of a SOFC stack based on this GA-RBF neural network identification model. 相似文献
73.
74.
Xiu-ping Lu Bing-guang Xiao Yong-ping Li Yi-jie Gui Yu Wang Long-jiang Fan 《Journal of Zhejiang University. Science. B》2013,14(7):570-577
Diversity arrays technology (DArT) is a microarray-based marker system that achieves high throughput by reducing the complexity of the genome. A DArT chip has recently been developed for tobacco. In this study, we genotyped 267 flue-cured cultivars/landraces, including 121 Chinese accessions over five decades from widespread geographic regions in China, 103 from the Americas, and 43 other foreign cultivars, using the newly developed chip. Three hundred and thirty polymorphic DArT makers were selected and used for a phylogenetic analysis, which suggested that the 267 accessions could be classified into two subgroups, which could each be further divided into 2–4 sections. Eight elite cultivars, which account for 83% of the area of Chinese tobacco production, were all found in one subgroup. Two high-quality cultivars, HHDJY and Cuibi1, were grouped together in one section, while six other high-yield cultivars were grouped into another section. The 330 DArT marker clones were sequenced and close to 95% of them are within non-repetitive regions. Finally, the implications of this study for Chinese flue-cured tobacco breeding and production programs were discussed. 相似文献
75.
GLABROUS INFLORESCENCE STEMS regulates trichome branching by genetically interacting with SIM in Arabidopsis 总被引:1,自引:0,他引:1
Li-li Sun Zhong-jing Zhou Li-jun An Yan An Yong-qin Zhao Xiao-fang Meng Clare Steele-King Yin-bo Gan 《Journal of Zhejiang University. Science. B》2013,14(7):563-569
Arabidopsis trichomes are large branched single cells that protrude from the epidermis. The first morphological indication of trichome development is an increase in nuclear content resulting from an initial cycle of endoreduplication. Our previous study has shown that the C2H2 zinc finger protein GLABROUS INFLORESCENCE STEMS (GIS) is required for trichome initiation in the inflorescence organ and for trichome branching in response to gibberellic acid signaling, although GIS gene does not play a direct role in regulating trichome cell division. Here, we describe a novel role of GIS, controlling trichome cell division indirectly by interacting genetically with a key endoreduplication regulator SIAMESE (SIM). Our molecular and genetic studies have shown that GIS might indireclty control cell division and trichome branching by acting downstream of SIM. A loss of function mutation of SIM signficantly reduced the expression of GIS. Futhermore, the overexpression of GIS rescued the trichome cluster cell phenotypes of sim mutant. The gain or loss of function of GIS had no significant effect on the expression of SIM. These results suggest that GIS may play an indirect role in regulating trichome cell division by genetically interacting with SIM. 相似文献
76.
Hong-dan Wang Chun-mei Shen Wen-juan Liu Yu-dang Zhang Guang Yang Jiang-wei Yan Hai-xia Qin Bo-feng Zhu 《Journal of Zhejiang University. Science. B》2013,14(6):533-540
We studied the allelic frequency distributions and statistical forensic parameters of 21 new short tandem repeat (STR) loci and the amelogenin locus, which are not included in the combined DNA index system (CODIS), in a Russian ethnic minority group from the Inner Mongolia Autonomous Region, China. A total of 114 bloodstain samples from unrelated individuals were extracted and co-amplified with four fluorescence-labeled primers in a multiplex polymerase chain reaction (PCR) system. Using capillary electrophoresis, the PCR products of the 21 STR loci were separated and genotyped. A total of 161 alleles were observed in the Russian ethnic minority group, and corresponding allelic frequencies ranged from 0.0044 to 0.5965. The 21 non-CODIS STR loci of the Russian ethnic minority group were characterized by high genetic diversity and therefore may be useful for elucidating the population’s genetic background, for individual identification, and for paternity testing in forensic practice. 相似文献
77.
78.
79.
80.
目的:考察苍术遗传变异与挥发油变异的关系,揭示苍术挥发油变异的生物学基础。方法:分单株取来源于多个居群的苍术,同株苍术根茎与叶片采用同一编号,根茎用GC—MS分析挥发油变异,叶片采用随机扩增多态性(RAPD)技术分析遗传变异,然后利用方差分析、聚类分析等分析二者的关系。结果:①不论在个体水平还是居群水平,苍术非道地药材与道地药材的差异显著,前者(居群Ⅰ和Ⅱ的亚居群1~5,样品1~36)主要由茅术醇和p-桉叶醇组分;后者(居群Ⅲ的亚居群6、7,样品37—47)主要由苍术酮和苍术素组成,并含有较多茅术醇和p-桉叶醇;②RAPD数据在居群水平的分析显示,苍术居群Ⅱ和Ⅲ聚类后,再与居群Ⅰ聚类。个体水平上,居群Ⅲ的全部11个个体聚为一类,亚居群5的5个个体聚类,其他苍术个体均为按居群或亚居群聚类。结论:不论在居群水平还是个体水平,苍术挥发油变异与遗传变异均呈现出特有的规律性,但二者并不一致,提示除了遗传因子外,环境因子对苍术道地药材的形成具有重要作用。 相似文献