论老子天道观思想对中国古典园林审美文化的影响

＂天人合一＂思想是中国传统文化的理论核心,是中华传统审美文化的灵魂,而老子的天道观思想是中国传统文化的＂天人合一＂思想之源头。＂天人合一＂思想对中国古典园林审美文化有着深刻的影响。     

人力资本理论对继续教育投资倾向的影响

纵观世界发展史,劳动力一直是经济学关注的生产要素,但近半个世纪以来劳动力才作为人力资本来系统地加以研究。人力资本理论从古典经济学中萌芽,经过马克思主义经济学和新古典经济增长理论的理论准备,最终促成舒尔茨从宏观层次研究并建立了人力资本理论。此后,贝克尔等人从中观层次做了大量的实证研究,而现代劳动经济学则更关心微观层次的实证研究,为继续教育的理性投资提供了更加科学的依据。     

汉、回民族4对遗传性状的调查——以河西学院为例

通过对河西学院汉、回民族学生的ABO血型、单双眼睑、耳垂有无及舌卷曲4对遗传性状的调查,比较了这4对性状的基因频率在两民族间的分布.结果表明:ABO血型、单双眼睑及耳垂有无3对遗传性状的基因频率在汉族和回族间存在明显差异,而舌卷曲基因频率在两民族间差异不大.     

对高职公共英语课时减少的思考

一方面公共英语课程在高职教育中有着举足轻重的作用,另一方面却面临其课时减少的尴尬困境。面对这一情况,我们要全面认识公共英语课程的作用和采取应对措施。     

维特根斯坦的人格瑕疵审视

哲学界从不同角度把维特根斯坦的人格瑕疵笼统地界定为:傲慢、苛求、粗暴、自私、多疑、悲观,并且以此贬损或撼动其伟大与崇高的哲学地位。从历史和现实的视角观之,维特根斯坦的人格瑕疵不但有其独特的历史背景,而且折射出他冲破哲学藩篱的人品光辉,具有治学严谨、和蔼可亲和乐善好施的美德。     

Danshen protects endothelial progenitor cells from oxidized low-density lipoprotein induced impairment

In this study,we examined the protective effects of Danshen both on endothelial progenitor cells(EPCs) in patients with hypercholesterolemia and on in-vitro EPCs of healthy volunteers.In the clinical study,we randomly divided 24 subjects with hypercholesterolemia into two groups(the control group and the Danshen-treated group).At the end of two weeks of treatment,the EPC cellular functions of both groups were tested.The results indicated that,compared to the control group,EPCs in the Danshen-treated group showed significantly better cellular functions,which was manifested in the cloning number,the proliferation capacity,the number of EPC adhesions,and cell migration.In the subsequent in-vitro experiments,EPCs were treated with vehicle,oxidized low-density lipoprotein(Ox-LDL,100 μg/ml),or Ox-LDL(100 μg/ml) plus different concentrations of Danshen(Danshensu 2,10,or 50 μg/ml,respectively) for 24 h.The results showed that Danshen treatments can prevent the detrimental effects of Ox-LDL on EPC cellular functions measured by proliferation capacity(0.24±0.08,0.37±0.11,0.30±0.04 vs.0.13±0.02,P0.05,P0.01,and P0.01,respectively),and adhesion ability(63.00±11.60,70.00±10.80,85.50±11.41 vs.40.50±6.85,all P0.01).Compared to the group treated with Ox-LDL alone,Danshen treatment significantly decreased the lipid peroxidation end product malondialdehyde(MDA) [(4.34±0.54),(3.98±0.47),(3.46±0.31) vs.(5.57±0.64) nmol/ml,all P0.01],increased the production of superoxide dismutase(SOD) [(29.74±0.71),(31.09±0.83),(30.41±0.65) vs.(14.76±3.99) U/ml,all P0.01],and lowered the expression of interleukin-6(IL-6) [(24.62±7.69),(27.04±3.14),(33.38±18.86) vs.(230.67±33.53) pg/ml,all P0.01] and tumor necrosis factor-α(TNF-α) [(41.72±6.10),(17.02±6.82),(3.73±2.26) vs.(228.71±41.53) pg/ml,all P0.01] in Ox-LDL treated EPCs.These results suggest that Danshen may exert a protective effect through its antioxidant and anti-inflammatory features.     

Effect of interrupted endogenous BMP/Smad signaling on growth and steroidogenesis of porcine granulosa cells

Bone morphogenetic proteins (BMPs) play a critical role in the growth and steroidogenesis of granulosa cells (GCs). BMP signals act through membrane-bound heteromeric serine/threonine kinase receptors. Upon ligand binding, BMPs activate intracellular Smad proteins and regulate growth and apoptosis in various cell types. The objective of this study was to demonstrate the effects of BMP/Smad signal on growth and steroidogenesis of porcine GCs. A strategy of RNA interference (RNAi)-mediated ‘gene silencing’ of Smad4, a core molecule mediating the intracellular BMP/Smad signal transduction pathways, was used to interrupt endogenous BMP/Smad signaling. Results indicate that Smad4-small interfering RNA (siRNA) caused specific inhibition of Smad4 mRNA and protein expression after transfection. Interrupted endogenous BMP/Smad signaling significantly inhibited growth, and induced apoptosis of porcine GCs, while decreasing estradiol production. In addition, interrupted BMP/Smad signaling significantly (P<0.05) changed the expression of Cyclin D2, CDK4, Bcl-2, and Cyp19a1. These findings provide new insights into how BMP/Smad signaling regulates the growth and steroidogenesis of porcine GCs.     

Arachnoid cell involvement in the mechanism of coagulation-initiated inflammation in the subarachnoid space after subarachnoid hemorrhage

Objective: To assess if arachnoid cells have the capability to present antigen and activate T-lymphocytes after stimulation by bloody cerebrospinal fluid (CSF), and to illuminate the mechanism of coagulation-initiated inflammation in the subarachnoid space after subarachnoid hemorrhage (SAH). Methods: Arachnoid cells were cultured, characterized, and examined by immunofluorescence for the basal expression of human leukocyte antigen-DR (HLA-DR). Expression of HLA-DR, after co-culturing arachnoid cells in vitro with bloody CSF, was investigated by immunofluorescence and flow cytometry (FCM). The variation of arachnoid cells' ultrastructure was observed by transmission electron microscope (TEM). Arachnoid cells were co-cultured with peripheral blood mononuclear cells (PBMCs). The content of soluble interleukin-2 receptor (sIL-2r) in culture medium was detected by enzyme-linked immunosorbent assay (ELISA). Results: (1) Arachnoid cells were successfully cultured for many passages. The immunofluorescent staining was positive for HLA-DR in over 95% of the human arachnoid cells. The punctate HLA-DR was distributed in cytoplasm and not in the karyon. (2) After co-culturing arachnoid cells in vitro with bloody CSF, numerous particles with strong fluorescence appeared in the cytoplasm on Day 6. On Day 8, the quantity of particles and fluorescent intensity were maximal. FCM showed that the percentage of HLA-DR expressing cells was (2.5±0.4)% at the first 5 d, increasing to (60.8±3.6)% on Day 7. (3) After co-culturing arachnoid cells in vitro with bloody CSF, many lysosome and secondary lysosome particles were present in the cytoplasm. Hyperplasia of rough endoplasmic reticulum and enlarged cysts were observed, with numerous phagocytizing vesicles also observed at the edge of the arachnoid cells. (4) Arachnoid cells stimulated by bloody CSF were co-cultured in vitro with PBMCs. The content of sIL-2r in the culture medium, having been maintained at around 1.30 ng/ml during the first 3 d, had increased by Day 4. The content of sIL-2r peaked 7.53 ng/ml on Day 7 and then reduced gradually. Conclusions: (1) Basic HLA-DR expression is present in arachnoid cells. (2) After stimulation by bloody CSF, arachnoid cells have the potential to serve as antigen presenting cells (APCs) and the ability to activate T-lymphocytes, indicating that arachnoid cells are involved in the mechanism of coagulation-initiated inflammation in the subarachnoid space after SAH.     

药品专利与人权的冲突与调适

近年来,随着全球化的推进、商品和人口的自由流动,各种传染性疾病快速蔓延于全球,人类对药品的需求也随之变得迫切。而由于对药品实施专利权的保护,导致药品价格居高不下,很多人由于经济原因而无力购买治疗疾病所需的基本药品,这与国际社会所大力提倡的人权保护显得格格不入。作为私权的药品专利权和作为人权的生命健康权之间存在着冲突与矛盾。在协调二者的关系上,药品专利制度本身的完善和新药品的研发是关键。     

对远程开放教育人力资源管理课程教学的思考与探索

远程开放教育人力资源管理课程的教学,应以培养学生自主学习的能力,掌握最基础的管理理论与实务,创造性的开展管理工作为核心。为经济建设培养既具备理论知识,又能学以致用的适用性人才,是远程开放教育人力资源管理教学的最终目标。