Allograftic bone marrow-derived mesenchymal stem cells transplanted into heart infarcted model of rabbit to renovate infarcted heart

Objective: To investigate the directed transplantation of allograftic bone marrow-derived mesenchymal stem cells (MSCs) in myocardial infarcted (MI) model rabbits. Materials and Methods: Rabbits were divided into 3 groups, heart infarcted model with MSCs transplanted treatment (MSCs group, n=12), heart infarcted model with PBS injection (control group, n=20), sham operation with PBS injection (sham group, n=l 7). MSCs labelled by BrdUrd were injected into the MI area of the MSCs group. The same volume of PBS was injected into the MI area of the control group and sham group. The mortality, LVIDd, LVIDs and LVEF Of the two groups were compared 4 weeks later. Tropomyosin inhibitory component (Tn I) and BrdUrd immunohistochemistry identified the engrafted cells 4 weeks after transplantation. Result: The mortality of the MSCs group was 16.7% (2/12), and remarkably lower than the control group's mortality [35% (7/20) (P＜0.05)].Among the animals that survived for 4 weeks, the LVIDd and LVIDs of the MSCs group after operation were 1.17±0.21 cm and 0.74±0.13 cm, and remarkably lower than those of the model group, which were 1.64±0.14 cm and 1.19±0.12 cm (P＜0.05); the LVEF of the MSCs group after operation was 63±6%, and remarkably higher than that of the model group,which was 53±6% (P＜0.05). Among the 10 cases of animals that survived for 4 weeks in the MSCs group, in 8 cases (80%),the transplanted cells survived in the non MI, MI region and its periphery, and even farther away; part of them differentiated into cardiomyocytes; in 7 cases (70%), the transplanted cells participated in the formation of blood vessel tissue in the MI region. Conclusion: Transplanted allograftic MSCs can survive and differentiate into cardiomyocytes, form the blood vessels in the MI region. MSCs transplantation could improve the heart function after MI.     

何杰金氏病肿瘤细胞生物学特征研究

目的对何杰金氏病肿瘤细胞进行形态学观察和免疫表型研究.材料方法福尔马林固定,石蜡包理的何杰金氏病组织12例(NSHL6例,MCHL5例,LDHL1例)进行HE染色和CD30、CD15、CD20、CD45RO、Ki-67抗原免疫组化在肿瘤细胞和背景成分中的表达情况.结果(1)R-S细胞有各种变体形式.(2)各型HL具有不同的背景成分和组织学特征.(3)NSHL肿瘤细胞表达CD30(6/6)、CD15(1/6),不表达CD20(0/6).MCHL肿瘤细胞表达CD30(5/5)、CD15(0/5),LDHL肿瘤细胞表达CD30(1/1).CD30阳性率明显高于CD15(0.01＜P＜0.025).结论(1)NSHL中纤维组织增生形成结节,瘤细胞以陷窝细胞为主;MCHL中诊断型R-S细胞及其它变体细胞数量较多;LDHL以淋巴细胞减少为其特征.均以表达CD30为其特征.(2)在何杰金氏病鉴别判断中一定要应用免疫组化.CD30、CD45RO、Ki-67、CD20一般为一线抗体.     

Induction of diabetes by Streptozotocin in rats

The objective of this study is to induce experimental diabetes mellitus by Streptozotocin in normal adult Wistar rats via comparison of changes in body weight, consumption of food and water, volume of urine and levels of glucose, insulin and C-peptide in serum, between normal and diabetic rats. Intra-venous injection of 60mg/kg dose of Streptozotocin in adult wistar rats, makes pancreas swell and at last causes degeneration in Langerhans islet beta cells and induces experimental diabetes mellitus in the 2–4 days. Induction of experimental diabetes mellitus is indeed the first step in the plan of purification of pancreatic Langerhans islet cells of normal rats for transplanting under the testis subcutaneous of experimentally induced diabetic rats. Streptozotocin induces one type of diabetes which is similar to diabetes mellitus with non-ketosis hyperglycemia in some animal species. For induction of experimental diabetes in male adult rats weighted 250–300 grams (75–90 days), 60mg/kg of Streptozotocin was injected intravenously. Three days after degeneration of beta cells, diabetes was induced in all animals. The diabetic and normal animals were kept in the metabolic cages separately and their body weight, consumption of food and water, urine volume, the levels of serum glucose, insulin and C-peptide quantities in all animals were measured and then these quantities were compared. For a microscopic study of degeneration of Langerhans islet beta cells of diabetic rats, sampling from pancreas tissue of diabetic and normal rats, staining and comparison between them, were done. Induction of diabetes with Streptozotocin decreases Nicotinamide-adenine dinucleotide (NAD) in pancreas islet beta cells and causes histopathological effects in beta cells which probably intermediates induction of diabetes. In this study, we used Streptozotocin for our experiments in induction of experimental diabetes mellitus. After Induction of diabetes, consumption of food and water, volume of urine and glucose increased in the diabetic animals in comparison with normal animals, but the weight of body and the volume of insulin and C-peptide decreased in the diabetic animals. Sampling and staining of pancreas tissue of diabetic and normal rats showed that the Langerhans islet beta cells of diabetic rats have been clearly degenerated. In three days, Streptozotocin makes pancreas swell and at last causes degeneration in Langerhans islet beta cells and induces experimental diabetes. It also changes normal metabolism in diabetic rats in comparison with normal rats. Consumption of water and food, volume of urine, serum glucose increases in diabetic animals in comparison with normal rats but the levels of serum insulin, C-peptide and body weight decreases.     

日本公共图书馆地方文献信息(历史照片数据)公开和信息公开化的现状与课题

本文就日本公共图书馆对历史照片、历史文献等人文资源的收集、保存、公开和信息公开化的情况与课题,研讨公共图书馆馆藏资料的电子化和信息公开化的必要性以及未来。迄今为止,日本公共图书馆的主要资料是纸质书籍。大部分公共图书馆尚未对图书馆资料进行电子化,而且也没有采购电子化资料。但是在公共图书馆收集的资料当中,尤其是对地方文献信息进行系统性收集、保存、公开和传承,是日本图书馆法对公共图书馆规定的主要任务之一。在这一点上,中国公共图书馆法也是一样的。保存、公开地方文献最合适的办法是电子化。所以地方文献信息必须要考虑电子化和在网络上公开。另外,在对照片资料进行电子化时除了照片本身,还应该对照片信息(照片年代、拍摄地点、照片内容等)进行登记。但是目前日本公共图书馆在公开历史照片时,对这些内容尚无统一规定。在此,我将介绍日本公共图书馆在历史照片、历史文献的电子化和信息公开化方面的现状,并探讨相关课题,以及对公共图书馆关于数字人文学进行展望。     

人性的复苏与抗争——论曹禺剧作中的女性形象兼及曹禺的女性观

曹禺剧作中的女性形象,大胆﹑叛逆﹑与众不同,曹禺把她们作为一个真正的“人”凸现出来,她们不再是男人的影子,而是像男人一样能够争取主动权、拥有独立人格的人,这也是曹禺不同于同时代作家的创作特点;但剧中女性人物的性格在她们悲剧性的人生历程中决不起主导作用,“情出于性”,所以,作者从情爱角度,将其投放在情感的三角关系中,用人性化的文笔剖析中国千百年来封建女性悲剧的实质——人性的复苏乃至与整个男权社会抗争造成的悲剧。     

Study of Resistin gene expression in peripheral blood mononuclear cell and its gene polymorphism in a small range population

INTRODUCTION Recently discovered as an endocrine organ, adi-pose tissue can secrete many cytokines such as Re-sistin, adiponectin and free fatty acids, and receives much attention in the study of insulin resistance and type 2 diabetes mellitus (T2DM). As a peptide hor-mone secreted by adipocyte, Resistin is considered to be the linkage between obesity and insulin resistance (Steppan et al., 2001). Most studies on Resistin in-vestigate adipose tissue. In this experiment we de-tected t…     

公正行政视阈中行政人员的角色定位

公正行政是构建社会主义和谐社会的前提条件和必然要求。行政人员只有在行政活动中不断反省自己所扮演的角色,通过否定“经济人”角色,肯定“公共人”角色和自觉实现“经济人”角色向“公共人”角色观念的转化,才能使自己的行政行为符合行政伦理道德,实现公正行政,促进和谐社会的构建。     

“人”、“文”的终极追求——中国佛教人文关怀的内涵

中国佛教人文关怀的内涵分为两个方面,即在人的内在方面,注重对于＂人＂本的弘扬,突出＂众生为本＂、＂以人为本＂和重视人的＂心＂,体现为对于众生的服务意识、对于人本身的生命意识和重视人的主观意志;在外在层面,注重对于＂文＂明的追求,分别从弃恶从善的道德追求、以和为贵的精神追求和人佛平等的境界追求等论述;＂人＂、＂文＂对现代和谐社会具有启示。     

人文地理实践教学体系构建与实现路径探讨

实践教学是高校地理学各专业人文地理课程教学的重要组成部分,薄弱的实践教学现状决定了人文地理课程教学改革的必然性。目前,在多重因素约束下,人文地理实践教学构建应遵循以人为本、量力而行、开放性等原则设计形成立体化实践教学体系,其实现路径包括科学制定培养方案、强化课堂实践教学、加强课程野外实习、完善课程论文写作、全面参与各种社会实践活动等。