对虾白斑综合症病毒囊膜蛋白Vp28基因的克隆、表达及蛋白纯化

以对虾白斑病毒DNA为模板扩增出Vp28基因,经限制性内切酶（SmaI,Not I）酶切后按正确的读码框顺序插入到pGEX-4T-2表达载体上,重组质粒转化大肠杆菌,经菌落PCR和质粒双酶切鉴定、序列测定确认,证实成功地构建了Vp28基因原核表达载体．转化菌经IPTG诱导表达,SDS-PAGE显示有与预期大小约52kD相吻合的融合蛋白带,18℃诱导24h后获得了可溶性表达的目的蛋白,采用Glutathione Sepharose 4B亲和层析对重组蛋白进行纯化,获得了纯度很高的目的蛋白．     

Funding higher education and wage uncertainty: Income contingent loan versus mortgage loan

We propose a simple theoretical model which shows how the combined effect of wage uncertainty and risk aversion can modify the individual willingness to pay for a HE system financed by an ICL or a ML. We calibrate our model using real data from the 1970 British Cohort Survey together with the features of the English HE financing system. We allow for individual heterogeneity by considering different family backgrounds and occupations. We find that graduates from poor families, males and graduates working in the private sector are more willing to pay to switch to an ICL. Using the UK Labour Force Survey we evaluate the distributive effects of our model. We compute the repayment burdens and taxpayer subsidies for average, low and high earnings graduates. The results confirm the important insurance benefits of an ICL compared to a ML, with lower burdens and higher subsidies for poorer graduates.     

Do teacher characteristics matter? New results on the effects of teacher preparation on student achievement

Research fairly consistently demonstrates that teachers are an important measurable factor in student learning, yet few teacher characteristics are shown to be consistently related to student achievement. Using a state administrative dataset that matches individual students to their teachers over time, I find that math teachers’ undergraduate performance, as measured by GPA (overall, math, and math education) and course hours (math and math education), is predictive of 5th grade math achievement. The effects of the teacher characteristics are mediated by years of experience and vary according to student types.     

浅谈数学教学的“生活味儿”

让数学走进生活是新课标的要求。让课堂生活化,引导学生在生活情境中感受数学,体验数学源于生活的真正含义,对提高数学教学效果具有事半功倍的作用。但同时切忌把数学生活化片面理解,而从一个极端走向另一个极端。     

课程建设与教材建设相结合对精神医学专业发展的促进效果研究

课程与教材建设是高校专业建设重要重要内容,如何有效发挥两者的协调促进作用是高等教育工作中的一项重要任务,精神医学专业在建设发展过程中以课程建设为基础,科学引导教材建设内容和方向,并以教材建设为篮板,促进课程体系改革和教学方法的完善,经多年的实践,我院的精神医学专业得到了蓬勃发展,为我国精神卫生事业的进步做出了积极的贡献.     

KYN28A-12型高压开关柜接地刀闸的改造

通过对KYN28-10型手车式开关柜接地刀闸隐患的分析,找出了原因,提出了解决问题的方法,保证了高压开关柜的安全可靠运行。     

《庄子》二题

庄子的“吾丧我”涵盖工夫与本体两个层面,其含义是“唯丧我才能见道”,又是“见道后的自然解脱”。庄子“大木”的形象是丑的,但“大木”意象是美的,“大木”的本质是庄子对个体生死意义的独到领悟,又是一种超然而可求的人生态度。     

投掷运动员体成分的调查分析

通过对受试者体成分皮褶厚度的测定,并对其派生指标的比较发现：投掷选手皮脂较厚,其中重器械投掷选手达到肥胖标准。分析认为,这是由于其肌肉体积逐渐增大的同时,总伴随着皮下脂肪逐渐堆积的结果。肥胖不仅影响运动的效果,对身体健康也不利。建议投掷选手在促使肌肉体积增大的同时应积极采取消除脂肪的措施。