“辣”、“倔”、“蛮”——丁玲个性气质的文化基因及其丰富、发展

丁玲的个性气质 ,可以归结为“辣”、“倔”、“蛮”三个字。她火辣、热情 ;她倔强、执着、乐观洒脱、刚毅勇悍、好胜任性 ;她又具有“霸蛮”的精神 ,吃得苦 ,耐得劳 ,不达目的决不罢休。这些个性气质既有父母的遗传基因 ,同时又是湖湘文化孕育的结果 ,是一系列环境因素造成的 ,是丁玲长期社会实践的产物     

人体基因的客体性质研究

人体基因是DNA分子上具有遗传信息的特定核苷酸序列。它与每一个个体都息息相关。目前对于人体基因的研究较多,但是对于人体基因在法律上的客体性质如何来界定还没有定论。本文在分析人体基因的基本生物学特征和作用的基础上着重分析人体基因研究的法律意义及人体基因的法律客体性质,认为在法律上人体基因不是一种物,也不是一般的隐私信息,而是自然人的最机密、最根本的人格信息。     

富硒酵母抗动物热应激的研究进展

热应激是热带、亚热带及温带地区影响畜牧业健康发展的最常见因素之一。许多国家积极采取多种措施防控热应激的发生。富硒酵母因其广泛、确实、高效的生物学特性广泛用于缓解动物热应激的研究。本文对富硒酵母在提高热应激动物生产性能、改善营养物质代谢和增强机体免疫力等方面的作用进行综述,为进一步开发利用富硒酵母缓解动物热应激提供研究基础。     

不同肥料处理下茶园土壤细菌和古菌群落的时间变化研究

研究目的：研究化学肥料和有机肥处理条件下,茶园酸性土壤细菌和古菌群落结构,以及氮素转化相关功能酶基因丰度的时间变化规律。 创新要点：研究肥料、土壤温度及土壤含水量对茶园酸性土壤细菌和古菌群落结构,以及氮素转化相关功能酶基因丰度的影响。 研究方法：应用末端限制性片段长度多态性（T-RFLP）技术分析茶园酸性土壤中细菌和古菌群落结构随时间的变化规律,应用荧光定量聚合酶链式反应（PCR）技术,研究茶园酸性土壤细菌、古菌、硝化作用功能酶基因（细菌和古菌amod基因）和细菌反硝化作用功能酶基因（narG、nirK、nirS和nosZ基因）丰度的时间变化规律。 重要结论：茶园土壤细菌和古菌群落结构受到肥料的影响,并随着取样时间有显著的变化。细菌、古菌和古菌的amoA基因的丰度在7月份最小,而细菌的amoA基因和反硝化作用功能酶基因（除nirK基因）的丰度在9月份最小。有机肥处理增加了细菌、古菌和氮素转化相关功能酶基因的丰度,但化学肥料的施用对菌群及功能酶基因丰度的影响较小。土壤温度显著影响了土壤细菌和古菌的群落结构。土壤含水量与细菌反硝化作用功能酶基因有显著的相关性。土壤有机碳含量与细菌、古菌及功能酶基因丰度之间有显著的相关性。     

Identification and characterization of profilin gene family in rice

BackgroundProfilin proteins (PRFs) are small (12–15 kD) actin-binding protein, which play a significant role in cytoskeleton dynamics and plant development via regulating actin polymerization. Profilins have been well documented in Arabidopsis, Zea mays L. as well as Phaseolus vulgaris, however no such fully characterization of rice (Oryza sativa L.) profilin gene family has been reported thus far.ResultIn the present study, a comprehensive genome-wide analysis of rice PRF genes was completed and three members were identified. OsPRF1 and OsPRF2 shared 98.5% similarity (6 nucleotide divergence), but the deduced amino acid sequences of OsPRF1 and OsPRF2 are fully identical. In contrast, the OsPRF3 presents relatively lower similarity with OsPRF1 and OsPRF2. Phylogenetic analysis also support that OsPRF1 has a closer relationship with OsPRF2. Expression pattern analysis revealed the differential expression of OsPRFs in tissues of mature plant, which suggested the potential spatial functional specificity for rice profilin genes. Subcellular localization analysis revealed the OsPRFs were localized in cytoplasm and nucleus and all of them could bind actin monomers. Furthermore, abiotic stresses and hormones treatments assay indicated that the three OsPRF genes could be differentially regulated, suggesting that OsPRF genes might participate in different stress processes in rice.ConclusionsTaken together, our study provides a comprehensive analysis of the OsPRF gene family and will provide a basis for further studies on their roles in rice development and in response to abiotic stresses.How to cite: Zhang Y, Dong G, Wu L, et al. Identification and characterization of profilin gene family in rice. Electron J Biotechnol 2021;54. https://doi.org/10.1016/j.ejbt.2021.08.004.     

爬梯、跑台运动对小鼠骨骼肌中细胞凋亡调控基因的影响

目的观察爬梯与跑台两种不同运动方式对小鼠骨骼肌中细胞凋亡调控基因的影响。方法将实验小鼠分为安静对照组(YC)、爬梯运动组(YR)和跑台运动组(YE),采用实时荧光定量PCR的方法,分别对各组腓肠肌中凋亡调控基因(ARC、Bc l-2、Bax、HSP70、X IAP)及Caspase-3基因进行检测。结果 (1)与YC组相比,YR组ARC和HSP70 mRNA水平均显著上调(P<0.05),而Bax和Caspase-3 mRNA水平显著下调(P<0.05);(2)与YC组相比,YE组Bc l-2、HSP70和X IAP mRNA水平均显著上调(P<0.05),而ARC和Bax mRNA水平均显著下调(P<0.05)。结论爬梯和跑台运动均可干预凋亡调控基因而弱化骨骼肌细胞凋亡的潜能。     

过度训练对大鼠红细胞免疫功能的影响

为了探讨过度训练对红细胞免疫功能的影响。采用红细胞免疫检测花环试验的方法，对红细胞的免疫功能进行了定性和定量分析。结果表明，第四周末，训练组红细胞C3b受体花环率(RBC-C3bRR)和红细胞免疫复合物花环率(RBC-ICR)与对照组比较，无显著性差异；第八周末，训练组RBC-C3bRR和RBC-ICR与对照组比较，显著下降。以上结果显示，过度训练对红细胞免疫有抑制作用。     

Immune disorders and dyslexia

The Geschwind-Behan-Galaburda hypothesis of cerebral lateralization postulates an association between immune disorders and learning disabilities. So far, the empirical evidence for such a relationship has been ambiguous. The present study is an attempt to investigate the hypothesis in a population where all the individuals were affected by immune disorders. Thus, 29 students in a special school for asthmatic children (total number of students in the school: 32) were investigated. A number of reading tests with special focus on word decoding were administered. The parents filled in a detailed questionnaire on the prevalence of reading difficulties and immune disorders among the other family members. The proportion of students with reading problems — especially phonological problems — was much higher than would be expected in a normal population. Among the family members we also found an elevated incidence of both reading problems and immune disorders. A cautious interpreptation of these findings leads to tentative and partial support of the Geschwind-Behan-Galaburda hypothesis.     

Application of a recombinant replicase to localize the Trionyx sinensis hemorrhagic syndrome virus and evaluate its effects on antiviral genes of T. sinensis

Trionyx sinensis Hemorrhagic Syndrome Virus(TSHSV) is an arterivirus newly discovered in Chinese softshell turtles. Little is known about the effect of antibodies against the virus or the distribution of the virus in different organs of infected turtles. In this study, a partial protein of TSHSV-HP4 was produced using a prokaryotic expression system, and its polyclonal antibody was generated. The polyclonal antibody was confirmed by western blot and dot enzyme-linked immunosorbent assay(dot-ELISA). The distribution of TSHSV in different organs of T. sinensis was examined by immunohistochemistry(IHC) and the expression of immune-related genes was analyzed using quantitative real-time polymerase chain reaction(qRT-PCR). The results indicated that the recombinant TSHSV-HP4 protein was successfully expressed, and the generated polyclonal antibody showed specific binding to viral particles in the lung tissues of infected turtles. The IHC assay indicated that the virus was highly localized in various cells, including intestinal lymphocytes,enterocytes, kidney epithelial cells, spleen cells, lung macrophages, and cardiomyocytes. The qRT-PCR analysis revealed that TSHSV was detected in all organs tested, including the lungs, liver, kidneys, spleen, and heart. The numbers of viral mRNA copies in lung and heart tissues were significantly higher in the virus-antibody group than in the virus group. The interferonstimulated genes(ISGs), myxovirus resistance protein 2(MX2) and radical S-adenosyl methionine domain containing 2(RSAD2) were highly upregulated in all groups of infected turtles. Antibody-dependent enhancement(ADE) seemed to occur after stimulation by the polyclonal antibody, because significantly greater expression of the two genes was detected in the virus-antibody group than in the virus group. Overall, these results are important in understanding the cell localization of TSHSV and the immune response of infected turtles.     

几丁质对肿瘤及免疫作用的实验研究

采用微晶几丁质，观察其对小鼠肿瘤的抑制作用及对免疫功能和细胞毒性的影响。结果显示：经灌胃途径给予剂量为４００ｍｇ／ｋｇ的几丁质制剂，具有延缓小鼠腹水瘤生和作用；对小鼠实体瘤Ｓ１８０生长具有抑制作用，其抑瘤率达３３％。研究还表明，几丁质对小鼠腹腔巨噬细胞的吞噬功能具有明显的促进作用；对人二倍体２ＢＳ细胞和小鼠骨髓瘤ＳＰ２／０细胞地直接