中国半边莲属山梗菜亚属的修订

本文以可收集到的标本，运用形象化散点图、柱状图等方法，对国产山梗菜亚属Subgen．Tupa E．Wimm．性状的变异幅度及相关性进行了统计分析,并结合花粉特征及种皮表面纹饰，对该亚属的国产种进行了修订，发现一新种(叶苞山梗菜L．foliiformis)一新亚种(贵州山梗菜L ．colorata subsp．guizhouensis)，同意廉永善对L ．hybrida的处理，并把L ．taliensis也归人L ．colorata，将后者分为三个亚种。对于L ．kwangsiensis我们的分析结果支持廉永善在《中国植物志》中的划分，给予降级，并置于L ．davidii内。     

Cu^2＋对水稻幼苗某些生理特性的影响

正常浸种后的水稻种子,在不同深度CuSO4处理下进行培养,结果表明L随着Cu62 浓度的增高,对种子的发芽率影响较小,但根的生长受到强烈抑制,芽的生长也受到影响,幼苗体内的淀粉酶活性下降,过氧化物酶活性却升高,脯氨酸含量亦增加。     

沙棘籽油中脂肪酸的分析

对沙棘籽油中脂肪酸的组成和含量进行分析．对沙棘籽油中脂肪酸进行甲酯化处理,采用气相色谱-质谱联用仪对脂肪酸进行分离和鉴定,分离鉴定出19种脂肪酸,脂肪酸的主要组成是不饱和脂肪酸,占脂肪酸的85．41％．用高效液相色谱法对亚油酸进行了定量分析．     

试论我省春小麦四级繁种程序

经过分析青海省春小麦良种“三圃制”生产存在的弊端,提出了四级繁种程序,并以影响品种群体遗传稳定性的四个主要方面了该生产程序在保持品种群体遗传稳定和真实性方面的理论。     

Response to temperature stress of reactive oxygen species scavenging enzymes in the cross-tolerance of barley seed germination

A number of studies have shown the existence of cross-tolerance in plants, but the physiological mechanism is poorly understood. In this study, we used the germination of barley seeds as a system to investigate the cross-tolerance of low-temperature pretreatment to high-temperature stress and the possible involvement of reactive oxygen species (ROS) scavenging enzymes in the cross-tolerance. After pretreatment at 0 °C for different periods of time, barley seeds were germinated at 35 °C, and the content of malondialdehyde (MDA) and the activities of ROS scavenging enzymes were measured by a spectrophotometer analysis. The results showed that barley seed germinated very poorly at 35 °C, and this inhibitive effect could be overcome by pretreatment at 0 °C. The MDA content varied, depending on the temperature at which seeds germinated, while barley seeds pretreated at 0 °C did not change the MDA content. Compared with seeds germinated directly at 35 °C, the seeds pretreated first at 0 °C and then germinated at 35 °C had markedly increased activities of superoxide dismutase (SOD), ascorbate peroxidase (APX), catalase (CAT), and glutathione reductase (GR). The SOD and APX activities of seeds germinated at 35 °C after 0 °C-pretreatment were even substantially higher than those at 25 °C, and GR activity was similar to that at 25 °C, at which the highest germination performance of barley seeds was achieved. These results indicate that low-temperature pretreatment can markedly increase the tolerance of barley seed to high temperature during germination, this being related to the increase in ROS scavenging enzyme activity. This may provide a new method for increasing seed germination under stress environments, and may be an excellent model system for the study of cross-tolerance.     

重金属铜、锌、铅对苦荞种子萌发的影响

铜（Cu）、锌（Zn）、铅（Pb）都是土壤重金属污染源,对土壤、植物、动物及人体产生巨大危害。本文以苦荞种子为实验材料,研究经不同浓度硫酸铜、硫酸锌和乙酸铅催芽后的苦荞种子发芽率、发芽势、发芽指数和活力指数的变化情况。结果表明：当硫酸铜、乙酸铅浓度大于100 mg/L时,苦荞种子发芽率、发芽势和发芽指数均会受到不同程度的抑制,不同浓度硫酸锌对苦荞种子的发芽率、发芽势及发芽指数均无影响。经3种不同浓度重金属盐催芽后的苦荞种子,幼苗胚根平均长度和种子的活力指数均随着重金属盐浓度的增加而降低,且三种重金属盐对其抑制效果的大小顺序为硫酸铜〉乙酸铅〉硫酸锌。     

外源ZLI对水稻萌发的生理效应

以不同浓度的植物生长调节剂(ZLI)处理水稻种子,分别测定其根系活力、淀粉酶活力及可溶性糖的含量。结果表明:不同浓度的ZLI对种子的萌发影响各不相同,当浓度为10-5g/L时,种子的根系活力、淀粉酶活力均明显提高,可溶性糖含量稍有下降。说明ZLI是一个剂量因子,浓度为10-5g/L可以促进种子的萌发。     

亚临界水萃取元宝枫种壳中单宁工艺条件优化初探

以元宝枫种壳为原料,采用亚临界水萃取技术探索单宁提取的最佳工艺条件。以单因素实验为基础,通过正交实验确定了元宝枫种壳中单宁提取的最佳工艺条件为：粉碎粒度为40-60日,萃取时间为60min．去离子水用量为18mL／g,萃取温度为106％。在此工艺条件下单宁的提取率为94．65％。经正交实验方差分析,粉碎粒度和萃取温度是影响单宁提取的关键因素。     

用RAPD方法鉴定杂交辣椒种子纯度

用ＲＡＰＤ（随机扩增多态性ＤＮＡ）方法对辣椒种子的父本、母本及杂种一代进行分析研究,筛选出适合辣椒杂种一代种子纯度鉴定的随机引物Ｓ５,并对其进行了种子纯度的初步鉴定．ＲＡＰＤ结果和田间鉴定的相比,在误差允许范围内,进一步证明了ＲＡＰＤ方法在蔬菜种子纯度鉴定中应用的可行性