“意”与“匠”——谈现代环境小品设计的创意与表达

过去的二十几年,我国环境建设不断升温,各类环境小品越来越多地出现在公众视野。该文以两个引发争议的环境小品案例分析了目前环境小品设计存在的创意和表达问题．并分别就这两方面做出一些思考探讨。     

新课程下语文阅读教学中“度”之探微

在目前语文阅读教学中,出现了一些把握“无度”的做法,不但影响教学效果,而且会影响语文课程改革的顺利推进。因此,切实把握阅读教学中的“度”,那就是：教学目标要适度;课堂提问要适度;多元解读要适度;拓展延伸要适度。     

康脑液对皮质区干细胞因子基因表达的影响

目的:在康脑液方剂干预下观察皮质区内源性神经细胞干细胞因子(SCF)mRNA在大鼠脑缺血再灌注损伤后不同时间的表达情况。方法:成年SD大鼠,以线栓法建立大脑中动脉缺血再灌注模型,随机分为模型组、药物干预组和假手术组。原位杂交技术检测脑缺血1.5h再灌注1～14d后,脑皮质区SCFmRNA表达情况。结果:脑缺血再灌注后,药物干预组、模型组SCFmRNA的表达在皮质区均明显高于假手术组,于第7天达高峰,第14天下降。结论:药物干预后脑缺血再灌注脑皮质区SCFmRNA表达在不同时间点与模型组具备相同的表达规律,两组SCFmRNA的表达无显著性差异。     

串通投标犯罪问题研究

通过分析论证刑法与招标投标法、建筑法之间的规律和特点,旨在强化与协调招标投标法与刑法在控制招标投标领域犯罪的进路。     

目标信息系统的依赖约简

以决策属性关于条件属性的依赖度为标准，提出了一种基于依赖度的约简概念，并讨论了相应的约简方法以及这种约简与已有的一些约简的关系。     

目标法教学在《电子线路》课堂教学中的应用

针对解决教师满堂灌,学生被动学的问题,提出了运用目标教学法,使学生对枯燥难懂的内容有了分层、分步的理解和掌握。     

Study on the expression and mutation of human telomeric repeat binding factor ( hTRF1 ) in 10 malignant hematopoietic cell lines

Objective: Detecting the expression and mutation of human telomeric repeat binding factor (hTRF1) in 10 malignant hematopoietic cell line cells on the base of determining its genomic structure and its four pseudogenes to clarify if hTRF1 mutation is one of the factors of the activation of telomerase. Methods: hTRF1cDNA sequences were obtained from GenBank, its genome structure and pseudogenes were forecasted by BLAST and other biology information programs and then testified by sequencing. Real-time RT-PCR was used to detect the expression of hTRF1mRNA in 10 cell line cells, including myelogenous leukemia cell lines K562, HL-60, U-937, NB4, THP-1, HEL and Dami; lymphoblastic leukemia cell lines 6T-CEM, Jurkat and Raji. Telomerase activities of cells were detected by using telomeric repeat amplification (TRAP)-ELISA protocol. PCR and sequencing were used to detect mutation of each exon of hTRF1 in 10 cell line cells. Results: hTRF1 gene, mapped to 8q13, was divided into 10 exons and spans 38.6 kb. Four processed pseudogenes of hTRF1 located on chromosome 13, 18, 21 and X respectively, was named as ψhTRF1-13, ψhTRF1-18, ψhTRF1-21 and ψhTRF1-X respectively. All cell line cells showed positive telomerase activity. The expression of hTRF1 was significantly lower in malignant hematopoietic cell lines cells (0.0338, 0.0108:_0.0749) than in normal mononuclear cells (0.0493, 0.0369:_0.128) (P=0.004). But no significant mutation was found in all exons of hTRF1 in 10 cell line cells. Four variants were found in part of intron 1, 2 and 8 of hTRF1. Their infection on gene function is unknown and needs further studies. Conclusion: hTRF1 mutation is probably not one of the main factors for telomerase activation in malignant hematopoietic disease. Project supported by the National Basic Research Program (973) of China (No. 2002CB713700) and the National Natural Science Foundation of China (No. 39870339)     

Transient expression of chicken alpha interferon gene in lettuce

We investigated the possibility of producing chicken alpha interferon (ChIFN-α) in transgenic plants.The cDNA encoding ChIFN-a was introduced into lettuce (Lactuca sativa L.) plants by using an agro-infiltration transient expression system.The ChIFN-α gene was correctly transcribed and translated in the lettuce plants according to RT-PCR and ELISA assays.Re-combinant protein exhibited antiviral activity in vitro by inhibition of vesicular stomatitis virus (VSV) replication on chicken embryonic fibroblast (CEF).The results demonstrate that biologically active avian cytokine with potential pharmaceutical ap-plications could be expressed in transgenic lettuce plants and that it is possible to generate interferon protein in forage plants for preventing infectious diseases of poultry.     

Family Communication Patterns as Predictors of Organizational Dissent: A Replication Study

This study replicated a previous research project addressing connections between family-of-origin communication and organizational dissent expression. We predicted that family communication patterns (FCPs), specifically conversation and conformity orientations, would predict upward, lateral, and displaced dissent. As in the original study, significant findings emerged for upward dissent and conformity orientation. This replication study indicated family type as an inconsistent predictor of expressed dissent. Contrary to the original study, we found no evidence to support a connection between pluralistic family types and upward dissent. Finally, as an extension of the original study we analyzed effects of FCPs on displaced dissent, finding an inverse association with conversation orientation. Implications for future research are discussed.     

“搞”的艺术化用法探略

阐述“搞”的艺术化用法及其修辞效果,澄清对“搞”字运用的某种误解。认为“搞”具有随意性、口语化、概括性等特点,实际运用中出现的适应特定语境之用法,含有亲切、谦虚、个性化等意味,能表达出各种复杂的意思,可以收到精炼简洁、轻松诙谐、容易理解等表达效果。有的带“搞”的说法还呈现凝固化、词汇化趋势,说明某些“搞”字在词语组合中具有不可替代的功用。