串通投标犯罪问题研究

通过分析论证刑法与招标投标法、建筑法之间的规律和特点,旨在强化与协调招标投标法与刑法在控制招标投标领域犯罪的进路。     

目标信息系统的依赖约简

以决策属性关于条件属性的依赖度为标准，提出了一种基于依赖度的约简概念，并讨论了相应的约简方法以及这种约简与已有的一些约简的关系。     

试论英语中委婉语及其表达

如何充分掌握利用委婉的表达是个很复杂的问题，启用何种委婉的说法会因人因时因地等不同的环境或条件而有所不同。作为一种修辞手段，委婉语还不仅仅是所谓“捡好听的说”，它与语言学中关于如何判定词义褒贬不无关系。实际上没有一种委婉的说法不打上交际者个人交际能力的烙印。本文重点讲了生活中常见的几种委婉的表达，也是外语教师教学中常常忽略的问题。     

基于营养动力学的两种群模型的最优控制

讨论了基于营养动力学的捕食．食饵模型的最优控制,得到了使资源管理者获得最大利润并使种群系统持续下去的最优控制率．     

大学生非理性行为探析

处于青年中期大学生 ,受生理和社会经济环境的影响 ,在社会交往中容易产生非理性行为。本文拟就大学生非理性行为的表现、原因及矫正大学生非理性行为的对策作一初浅探析 ,以引导大学生理智克服非理性行为     

目标法教学在《电子线路》课堂教学中的应用

针对解决教师满堂灌,学生被动学的问题,提出了运用目标教学法,使学生对枯燥难懂的内容有了分层、分步的理解和掌握。     

Study on the expression and mutation of human telomeric repeat binding factor ( hTRF1 ) in 10 malignant hematopoietic cell lines

Objective: Detecting the expression and mutation of human telomeric repeat binding factor (hTRF1) in 10 malignant hematopoietic cell line cells on the base of determining its genomic structure and its four pseudogenes to clarify if hTRF1 mutation is one of the factors of the activation of telomerase. Methods: hTRF1cDNA sequences were obtained from GenBank, its genome structure and pseudogenes were forecasted by BLAST and other biology information programs and then testified by sequencing. Real-time RT-PCR was used to detect the expression of hTRF1mRNA in 10 cell line cells, including myelogenous leukemia cell lines K562, HL-60, U-937, NB4, THP-1, HEL and Dami; lymphoblastic leukemia cell lines 6T-CEM, Jurkat and Raji. Telomerase activities of cells were detected by using telomeric repeat amplification (TRAP)-ELISA protocol. PCR and sequencing were used to detect mutation of each exon of hTRF1 in 10 cell line cells. Results: hTRF1 gene, mapped to 8q13, was divided into 10 exons and spans 38.6 kb. Four processed pseudogenes of hTRF1 located on chromosome 13, 18, 21 and X respectively, was named as ψhTRF1-13, ψhTRF1-18, ψhTRF1-21 and ψhTRF1-X respectively. All cell line cells showed positive telomerase activity. The expression of hTRF1 was significantly lower in malignant hematopoietic cell lines cells (0.0338, 0.0108:_0.0749) than in normal mononuclear cells (0.0493, 0.0369:_0.128) (P=0.004). But no significant mutation was found in all exons of hTRF1 in 10 cell line cells. Four variants were found in part of intron 1, 2 and 8 of hTRF1. Their infection on gene function is unknown and needs further studies. Conclusion: hTRF1 mutation is probably not one of the main factors for telomerase activation in malignant hematopoietic disease. Project supported by the National Basic Research Program (973) of China (No. 2002CB713700) and the National Natural Science Foundation of China (No. 39870339)     

汉字形体变化影响形声字"声符"表意

形声字“声符”表意日益复杂化的另一促成因素,是形声字本身形体结构的变化“。声符”书写变式、变体“,声符”形体改换、借用“,声符”形近,声近混误,讹传的形声字“的声符”误认,或文字归并,致使形声字“的声符”异字同体,因而产生庞大“的共用同一声符”的形声字字群,从而搅乱了形声字“声符”表意的直接性,有序性,往往造“成声符”表意的隐晦,游离。     

Transient expression of chicken alpha interferon gene in lettuce

We investigated the possibility of producing chicken alpha interferon (ChIFN-α) in transgenic plants.The cDNA encoding ChIFN-a was introduced into lettuce (Lactuca sativa L.) plants by using an agro-infiltration transient expression system.The ChIFN-α gene was correctly transcribed and translated in the lettuce plants according to RT-PCR and ELISA assays.Re-combinant protein exhibited antiviral activity in vitro by inhibition of vesicular stomatitis virus (VSV) replication on chicken embryonic fibroblast (CEF).The results demonstrate that biologically active avian cytokine with potential pharmaceutical ap-plications could be expressed in transgenic lettuce plants and that it is possible to generate interferon protein in forage plants for preventing infectious diseases of poultry.