BBO晶体光参量放大研究

The noncollinear optical parametric amplification in BBO crystal is theoretically investigated. The phase matching angle, gain bandwidth, optimal noncollinear angle and conversion efficiency for both type-Ⅰ and type-Ⅱ BBO are simulated. The numerical simulation results are important to the practical optical parametric amplification experiments with BBO crystal.     

宽带拉曼放大系统传输特性研究

这是一种用于计算宽带拉曼放大系统传输特性的新方法,综合考虑了色散效应、自相位调制(SPM)、交叉相位调制(XPM)等非线性效应,以及泵浦泵浦、信号信号、泵浦信号之间的受激拉曼散射效应(SRS)和放大的自发辐射噪声(ASE)的影响.作为仿真算法的一个应用,对32×10Gb/s的多波长泵浦的拉曼放大器的四个级联传输系统进行了模拟,比较了不同入射功率对接收性能的影响.     

SRY基因的分子机制及其在奶牛性别控制中的应用

综述了性别决定的生物学机制和性别决定基因（SRY）的定位、结构、功能、表达特异性及其作用的分子机制,在此基础上阐述了SRY在奶牛精子性别控制与胚胎性别鉴定中的应用,着重介绍了用于胚胎鉴定的PCR扩增法和DNA探针法。     

Classrooms for Children with Developmental Disabilities: Sound‐field and public address amplification systems compared

Background noise poses adverse effects on speech sounds and affects student learning, especially for children with developmental disabilities. Sound‐field and public address amplification systems can help to solve this problem by amplifying speech sounds relative to background noise. This study surveyed school classrooms for children with special needs, and compared the performance of a sound‐field and a portable public address system in classroom environments. Unoccupied room noise levels and reverberation times were measured in eight classrooms at four Hong Kong schools for children with special needs. Speech levels in each classroom were measured under three conditions: without amplification, with public address system amplification, and with sound‐field amplification. Speech‐to‐noise ratios were calculated for each condition. Noise and unamplified speech‐to‐noise ratio values exceeded recommended acoustic standards in all classrooms. When sound‐field and public address amplification systems were installed, speech‐to‐noise ratios improved considerably. When either amplification system was used, a uniform sound‐field resulted. The applicability of both types of amplification system and their relative merits in special education classrooms are discussed.     

快速纯化高活力基因工程Taq DNA聚合酶

用含有TaqDNA聚合酶基因的pTaq表达质粒转化E.coli DH5α菌株，IPTG诱导表态TaqDNA聚合酶。利用该酶的热，经两轮－70℃深度冷冻和75℃水浴，细菌裂解释放内容物，以高速离心除去冻融变性的细胞碎片及核酸蛋白的复合以达到快速纯化Taq DNA聚合酶的目的。PCR扩增反应表明所制备的Taq DNA聚合酶的活力、敏感性、特异性均达到试验要求。该方法具有快速简便的优点。     

MGB probe assay for rapid detection of mtDNA11778 mutation in the Chinese LHON patients by real-time PCR

Objective:Leber's hereditary optic neuropathY (LHON)is a maternally inherited degeneration of the optic nerve caused by point mutations of mitochondrial DNA(mtDNA).Many unsolved questions regarding the penetrance and pathophysiological mechanism of LHON demand efficient and reliable mutation testing.This study aims to develop a minor groove binder(MGB) probe assay for rapid detection of mtDNA11778 mutation and heteroplasmy in Chinese LHON patients by real-time polymerase chain reaction(PCR).Methods:Forty-eight patients suspected of having LHON and their maternal relatives underwent a molecular genetic evaluation,with 20 normal individuals as a control group at the same time.A real-time PCR involving two MGB probes was used to detect the mtDNA 11778 mutation and heteroplasmy.A linear standard curve was obtained by pUCmLHONG and pUCmLHONA clones.Results:All 48 LHON patients and their matemal relatives were positive for mtDNA 11778 mutation in our assay,27 heteroplasmic and 21 homoplasmic.Eighteen cases did not show an occurrence of the disease,while 9 developed the disease among the 27 heteroplasmic mutation cases.Eleven did not show an occurrence of the disease,while 10 cases developed the disease among 21 homoplasmic mutation cases.There was a significant difierence in the incidence between the heteroplasmic and the homoplasmic mutation types.The time needed for running a real-time PCR assay was only 80 min.Conclusion:This real-time PCR assay is a rapid,reliable method for mtDNA mutation detection as well as heteroplasmy quantification.Detecting this ratio is very important for predicting phenotypic expression of unaffected carriers.     

SINGLE CELL DEGENERATE OLIGONUCLEOTIDE PRIMER-PCR AND COMPARATIVE GENOMIC HYBRIDIZATION WITH MODIFIED CONTROL REFERENCE

ＩＮＴＲＯＤＵＣＴＩＯＮＦｌｕｏｒｅｓｃｅｎｃｅｉｎ ｓｉｔｕｈｙｂｒｉｄｉｚａｔｉｏｎ (ＦＩＳＨ )ａｎｄｐｏｌｙｍｅｒａｓｅｃｈａｉｎｒｅａｃｔｉｏｎ (ＰＣＲ)ｈａｄｂｅｅｎｓｕｃｃｅｓｓｆｕｌｌｙｕｓｅｄｆｏｒｐｒｅｉｍｐｌａｎｔａｔｉｏｎｇｅｎｅｎｔｉｃｄｉ ａｇｎｏｓｉｓ (ＰＧＤ ) (Ｖｅｒｌｉｎｓｋｙｅｔａｌ.,1 997,Ｓａｂｔａｌｏｅｔａｌ.1 995)ａｌｔｈｏｕｇｈｔｈｅｙｎｏｒｍａｌｌｙｏｎｌｙｏｆｆｅｒｉｎｆｏｒｍａｔｉｏｎｆｒｏｍｏｎｅｏｒｆｅｗｃｈｒｏｍｏｓｏｍｅｒｅ ｇｉｏｎｓｏｒｓｐ…     

聚合酶链式反应

介绍了分子生物学前沿技术聚合酶链式反应（PCR）的原理、方法及应用。     

猴源溶组织内阿米巴原虫巢式PCR检测方法的建立

目的:建立猴源溶组织内阿米巴原虫巢式PCR检测方法并初步应用于临床检测;方法:根据溶组织内阿米巴原虫16S r DNA基因序列,设计1对阿米巴属原虫保守引物和1对溶组织内阿米巴原虫特异性引物,建立猴源溶组织内阿米巴原虫巢式PCR检测方法,摸索出了该检测方法的最佳反应条件,进了行特异性和敏感性试验,并对96份猕猴粪便样品进行检测;结果:该巢式PCR方法能特异性地扩增出猴源溶组织内阿米巴原虫目的片段,与莫氏内阿米巴、波氏内阿米巴、迪斯帕内阿米巴、大肠内阿米巴、微小隐孢子虫等11种相关原虫基因组DNA无交叉反应;最低能检测到0.3 fg的阳性参照DNA;对临床样品检测结果表明该PCR技术检查阳性者显微镜检查均为阳性;结论:建立的巢式PCR方法具有高度的特异性和敏感性,对于溶组织内阿米巴原虫病的诊断和流行病学调查具有重要的应用价值。