PglnA启动子控制的ω-3去饱和酶基因的转基因集胞藻的构建

为了能在常温下提高多不饱和脂肪酸产量,以及用单交换的方式在集胞藻PCC6803的染色体上整合入基因片段,本研究首先构建出链霉素抗性基因、常温启动子、desB基因片段依次相连的质粒,然后以单交换的方式将这些片段整合在集胞藻Pcc6803的染色体上,最后成功筛选出了转基因藻.该研究为常温下大量表达多不饱和脂肪酸奠定了基础.     

匀鞭蚜小蜂(Encarsia sp.)扫描电镜观察 Ⅰ.成虫足的形态及感器

匀鞭蚜小蜂3对足的特点是:3对足的基节均具有相同数量的指状感器,转节上具有同样的盘状感器。前足胫节及基跗节发达,并有触角清洁器。后足腿节粗壮,便于跳跃。     

过滤海水培养异胶藻

本文根据过滤海水培养异胶藻的试验，探讨了在海产双壳类人工育苗过程中，采用经济、简便的过滤海水培养异胶藻的方法及在生产上运用的可能性．     

Improved elastase production by Bacillus sp.EL31410--further optimization and kinetics studies of culture medium for batch fermentation

An efficient culture medium producing a bacterial elastase with high yields was developed further following preliminary studies by means of response surface method. Central composite design (CCD) and response surface methodology were applied to optimize the medium constituents. A central composite design was used to explain the combined effect of three medium constituents, viz, glucose, K2HPO4, MgSO4@7H2O. The strain produced more elastase in the completely optimized medium, as compared with the partially optimized medium. The fitted model of the second model, as per RSM,showed that glucose was 7.4 g/100 ml, casein 1.13 g/100 ml, corn steep flour 0.616 g/100 ml, K2HPO4 0.206 g/100 ml and MgSO4@7H2O 0.034 g/100 ml. The fermentation kinetics of these two culture media in the flask experiments were analyzed. It was found that the highest elastase productivity occurred at 54 hours. Higher glucose concentration had inhibitory effect on elastase production. At the same time, we observed that the glucose consumption rate was slow in the completely optimized medium, which can explain the lag period of the highest elastase production. Some metal ions and surfactant additives also affected elastase production and cell growth.     

Improved elastase production by<Emphasis Type="Italic">Bacillus</Emphasis> sp. EL31410 —Further optimization and kinetics studies of culture medium for batch fermentation

An efficient culture medium producing a bacterial elastase with high yields was developed further following preliminary studies by means of response surface method. Central composite design (CCD) and response surface methodology were applied to optimize the medium constituents. A central composite design was used to explain the combined effect of three medium constituents, viz, glucose, K₂HPO₄, MgSO₄·7H₂O. The strain produced more elastase in the completely optimized medium, as compared with the partially optimized medium. The fitted model of the second model, as per RSM, showed that glucose was 7.4 g/100 ml, casein 1.13 g/100 ml, corn steep flour 0.616 g/100 ml, K₂HPO₄ 0.206 g/100 ml and MgSO₄·7H₂O 0.034 g/100 ml. The fermentation kinetics of these two culture media in the flask experiments were analyzed. It was found that the highest elastase productivity occurred at 54 hours. Higher glucose concentration had inhibitory effect on elastase production. At the same time, we observed that the glucose consumption rate was slow in the completely optimized medium, which can explain the lag period of the highest elastase production. Some metal ions and surfactant additives also affected elastase production and cell growth. Project (No. 300024) supported by the Natural Science Foundation of Zhejiang Province, China     

试论大学体育教学中的心理健康教育

通过对大学体育教学中学生心理障碍的分析，强调了开展心理健康教育的重要性，并提出了相应对策，以提高体育教学效果。     

THE GENERATING THEORY OF THE FLUTES''''PROFILE OF SPECIAL REVOLVING TOOLS WITH CONSTANT-SPIRAL-ANGLED EDGES

The manafacturing theory proposed in this paper is composed of the se-lection of the machining type(motions and adjustments)and the determination of thefunctions of motion parameters.The paper shows that the special revolving tools withconstant spiral angled egdes can be ground in the[x y (?)]or[x y z (?)]systembased on the conjugate theory and differential geometry.The functions of motion pa-rameters in these two systems are deduced.     

油茶组织培养研究进展

本文对目前我国油茶的组织培养研究现状进行总结,从培养条件、培养基、外植体类型、外源激素等方面分析油茶组织培养的特点和影响因素,提出油茶组织培养的研究方向及组织培养技术应用中注意的问题。     

高校体育股份制俱乐部系统管理模式的构建

从系统科学的角度出发，运用管理学方法，构建了高校体育股份制俱乐部的系统管理模式，从而有效解决高校体育教学和高校体育的社会化问题，形成科学的“学校—社区—社会”一体化的全面健身服务体系。     

慢性乙型肝炎病毒感染学生体育锻炼的现状

运用问卷调查法对高校慢性乙肝感染学生的体育锻炼意识、目的、行为和技能进行了调查分析,结果表明,慢性乙肝病毒感染学生担心体育锻炼损伤身体,体育锻炼意识及达标率明显低于正常学生,体育锻炼目的主要是应付考试。有必要对这些学生加强卫生宣传教育,改革教学方法。