毛细管电泳电化学检测法测定连翘中的芦丁

采用毛细管电泳电化学检测法测定了连翘中的芦丁.考察了工作电极的氧化电位、分离电压和进样时间对分离和检测的影响.在优化条件下,以300μm直径的碳圆盘电极为检测电极,检测电位为+0.9 V(vs.SCE),在50 mmol/L硼酸盐(pH8.4)的运行缓冲液中,被测物浓度与峰电流在三个数量级范围内呈良好线性,检测限为2×10-7g/mL.方法有着良好的重现性,被测组分的迁移时间和峰高的相对标准偏差(RSDs)小于4%(n=7).该法简单、可靠、快速,单次测定可在16 min完成,已经成功的应用于实际样品中芦丁的测定,样品处理简单,无须预富集,检测结果令人满意.①     

男子30km跑后尿液蛋白质组差异性表达的研究

目的:从蛋白质组学整体的角度,探讨30 km跑对尿液蛋白质组图谱差异性表达的影响,筛选对运动应激具有重要意义的目标蛋白。方法:以参加全国绿道长跑挑战赛30 km跑的10名男子为研究对象,分别留取运动前(晨)、后尿液,应用双向凝胶电泳确立运动前后尿液蛋白质组的差异性表达图谱,选取运动前后差异性表达量上调≥5倍和新增的蛋白点10个,用基质辅助激光解吸附电离串联飞行时间质谱仪进行质谱鉴定。结果:30 km跑前、后尿液2-DE图谱平均检测到蛋白质点(1011±243)和(1737±15)个,共获得差异性表达蛋白点110个,上调与下调各55个。其中运动后上调≥5倍的蛋白质点10个,新增点23个;运动后下调≥5倍的蛋白质点18个,消失点6个。对目标蛋白进行质谱分析,共鉴定出6个蛋白点,它们分别是锌α2-糖蛋白、白蛋白、维生素D结合蛋白、前列腺特异性抗原、β-肌动蛋白和本斯-琼斯蛋白,上述蛋白分别与机体能量代谢方式的转变、物质的转运、应激保护有关。结论:30 km跑引起尿液蛋白质组表达显著差异,ZAG、Alb、VDB、PSA、β-Actin、BJP的差异表达可作为运动营养补剂的筛选、运动负荷评价、运动性疲劳及肌损伤诊断的潜在目标蛋白;尿液蛋白图谱可作为运动员血液生物护照的补充用于反兴奋剂检测,实现监测无创化。     

尾悬吊大鼠股直肌废用性肌萎缩的相关蛋白分析

目的:通过双向电泳技术分离骨骼肌蛋白质组份,探讨悬吊后肌萎缩组股直肌与正常骨骼肌的差异蛋白。方法:建立大鼠尾悬吊模型,使大鼠后肢去负荷,两周后取大鼠股直肌进行双向电泳技术分离,使用PDQuest软件对凝胶图像进行差异蛋白组学分析。结果:研究发现在悬吊所致肌萎缩后有23个点发生了明显而稳定的改变,其中下调的蛋白有18个,上调的有5个。结论:悬吊致肌萎缩后,股直肌收缩蛋白、骨架蛋白、运输蛋白含量下调,而与代谢有关的蛋白或酶的表达有升有降,表现了在肌萎缩过程中代谢调节的复杂性。     

纳米TiO2对染料敏化纳米薄膜太阳电池的影响

在染料敏化纳米薄膜太阳电池中,纳米TiO₂ 是重要的组成物质之一.用溶胶 凝胶法制备纳米TiO₂的过程中,为了控制纳米TiO₂ 的大小及晶型采用了一系列方法.主要介绍热处理方法及实验结果.随着热处理温度的升高,纳米TiO₂ 的晶粒度随着长大.而且当水解pH～1,热处理温度达到 2 70℃时就已经有 43 %的金红石相纳米TiO₂出现.通过计算发现,其中金红石相纳米TiO₂ 比锐钛矿相纳米TiO₂ 的晶粒度大得多.将制备的纳米TiO₂ 应用于染料电池,通过太阳电池的测试实验证实,合适的热处理温度可得到较好的光电转换效率     

钛凝胶与二氧化钛粉末的光致变色

在实验基础上研究了钛凝胶和二氧化钛粉末在盐酸－－正丁醇体系的光致变色现象利用光谱仪对两种产物的光致变色特点进行了比较和分析。并提出了钛凝肽和TiO2粉末光致变色原理。     

醋酸纤维素膜电泳实验条件的探讨

针对血清蛋白醋酸纤维素膜电泳实验教学中存在的电泳图谱分离区带过窄而欠清晰的现状,分析和改进其实验条件,得出0.07mol·L-1缓冲溶液、200V电场强度、每厘米膜宽电流强度0.4-0.7mA和35分钟电泳时间的结果,提高了实验教学效果.     

毛细管电泳中固定套管式电极定位装置的改进

研究采用固定套管式电极调节定位方式,实现了工作电极与毛细管检测端口的完美对接。将两石英玻璃套管(内径400μm)经精确准直后固定在检测池中,工作电极由细微的石英玻璃毛细管(外径375μm,内径200μm)制作而成,工作时只需将制作的工作电极和电泳毛细管(石英玻璃质,外径375μm)分别插入套管,在两套管(间距约3 mm)中部轻微接触对接即可。该方法定位精确,操作简单方便,工作效率高,检测重现性好,较目前市面上部分使用的三维自由调节方式和螺杆调节方式的检测池有明显的优势和实用意义。     

铅离子诱导超螺旋DNA断裂的研究

利用琼脂糖凝胶电泳研究了不同浓度的铅离子对准X174RFDNA超螺旋结构的影响.铅离子浓度越高,对应的DNA超螺旋百分含量-时间曲线随时间变化越明显.实验结果表明铅离子浓度为5×10-4mol/L时,超螺旋DNA结构被破坏,超螺旋形式所占的百分含量与对应的作用时间具有相关性.     

Gamma heavy chain disease in a patient with rheumatoid arthritis – a laboratory evaluation

Introduction:

Heavy chain diseases (HCD) are neoplastic proliferations of B cells which secrete truncated immunoglobulin heavy chains without associated light chains. Being rare and probably underdiagnosed diseases the aim of this report is to show an additional case of gamma heavy chain disease in a 48 year old female patient with rheumatoid arthritis focusing on the laboratory presentation.

Materials and methods:

Laboratory work-up included agarose gel electrophoresis (AGE), capillary zone electrophoresis (CZE), immunofixation and nephelometrically determined immunoglobulin and immunoglobulin subclasses of the patient’s serum. Urine samples were also subjected to immunofixation and to a SDS-PAGE with consecutive immunoblot.

Results:

Nephelometrically measured elevated IgG concentrations were noted in combination with a decreased gamma globulin region and an increased beta globulin region on AGE. A definite monoclonal spike was not identified on AGE but at least suspected on CZE; finally serum and urine immunofixation demonstrated a monoclonal gamma heavy chain devoid of any corresponding light chains confirming the diagnosis of HCD. Analysis of the gamma heavy chain (HC) with means of SDS-PAGE revealed proteins of 40 kD and 80 kD most likely presenting a truncated HC in its monomeric and dimeric form and possibly leading to the failure of IgG-subclass typing with the applied IgG subclass antisera.

Conclusion:

This case report illustrates a new case of gamma HCD demonstrating variable laboratory manifestations and therefore the need for heightened awareness concerning this disease when confronted with abnormal and discrepant protein profiles in routinely applied laboratory tests.     

Characterization and separation of DNA complexes containing poly-L-lysine with or without the modification of PEG by capillary electrophoresis

The characterization of complexes is particularly critical for quality control and development of gene delivery systems. Here, the method of capillary zone electrophoresis (CZE) for the characterization of DNA and poly-L-lysine (MW 28 500) or DNA and poly-L-lysine modified with polyethylene glycol (MW10000) complexes at various charge ratios in phos-phate buffer is described firstly. During the characterization, DNA complexes can be separated into various components with different charge-to-mass ratio, i.e, components with single physicochemical property. And also the size and zeta potential of complexes were characterized by using photon correlation spectroscopy. This method is useful to characterize various com-plexes formed by DNA and polycations, and has the potential to separate complexes into homogeneous component for better transfection efficiency in vitro and in vivo in future.