无水溶胶凝胶法制备LiFe1-xCoxPO4/C正极材料及其性能研究

在无水溶胶凝胶法基础上,以Fe3+盐为铁源,添加聚乙二醇-400作为表面活性剂,制备了掺杂钴的LiFePO4/C正极材料(LiFe1-xCoxPO4/C,x=0,0.05,0.1)。并通过XRD、SEM、恒流充放电等方法研究了不同钴掺杂量对LiFePO4/C结构、形貌和电化学性能的影响。     

葡聚糖为筛分介质的无胶筛分毛细管电泳相互作用分析

本文利用线性高分子聚合物葡聚糖对大分子物质的筛分作用,通过改变大分子蛋白的电泳淌度,扩大迁移时间窗口,将无胶筛分毛细管电泳成功地运用于相互作用分析中,测定了伊贝沙坦与牛血清白蛋白之间的结合常数,建立了测定具有相似淌度的药物与蛋白之间结合常数的模型,进一步发展了毛细管电泳相互作用分析方法.     

高效毛细管电泳在手性药物拆分中的应用

介绍了高效毛细管电泳用于手性化合物拆分的主要模式,探讨了影响分离结果的主要因素,综述了各类手性选择剂的研究现状及在手性药物拆分中的应用.     

毛细管电泳电化学检测法测定荞麦中的多酚

采用毛细管电泳电化学检测法同时测定了荞麦的表儿茶素、芦丁、金丝桃甙和槲皮素的含量.考察了电极电位、运行缓冲液的酸度和浓度、电泳电压及进样时间对分离和检测的影响.在最佳实验条件下,以直径为300μm的碳圆盘电极为检测电极,检测电位为 0.90 V(υs.SCE),在50 mmol/L硼酸盐(pH8.7)的运行缓冲液中,上述各组分在20 min内能完全分离.被测物浓度与峰电流在三个数量级范围内呈良好线性,检测限在1×10-7g/ml~5×10-7g/ml之间,该法简单可靠,已经成功的应用于荞麦多酚的测定.yh     

Science Letters： Proteomic analysis of the serum in patients with idiopathic pulmonary arterial hypertension

Idiopathic pulmonary arterial hypertension （IPAH） is a rare disease of unknown etiology. The exact pathogenesis of pulmonary arterial hypertension is still not well known. In the past decades, many protein molecules have been found to be involved in the development of IPAH. With proteomic techniques, profiling of human plasma proteome becomes more feasible in searching for disease-related markers. In present study, we showed the protein expression profiles of the serum of IPAH and healthy controls after depleting a few high-abundant proteins in serum. Thirteen spots had changed significantly in IPAH compared with healthy controls and were identified by LC-MS/MS. Alpha- 1-antitrypsin and vitronectin were down-regulated in IPAH and may be valuable candidates for further explorations of their roles in the development of IPAH.     

磷酸化氨基酸分析进展

蛋白磷酸化的作用与功能研究日渐成为细胞生物学和生物医药科学的焦点,测定磷酸化蛋白质和磷酸化氨基酸对深入了解和研究细胞生物学和生物医学具有十分重要的意义。本文重点阐述了液相色谱-荧光检测和毛细管电泳-激光诱导荧光检测在分析磷酸化氨基酸方面的应用,并对两种方法进行了比较。     

醋豆小分子肽的提取与分析

目的：提取醋豆小分子多肽,分析降解的小分子肽的分子量分布谱及其意义。方法：用磷酸缓冲液提取小分子肽,用Tris-Tricine-SDS-PAGE凝胶电泳分析醋浸后蛋白质的降解。结果：大豆用醋和乙酸浸泡后,分子量大于66.4KD的均已降解,醋及乙酸浸泡液中几乎不含6.5KD以上的多肽,凝胶浓度为20%,并加入80%的甘油后,可见Marker清晰的9条带,低分子量可溶性多肽在这种条件下可以得到比较清晰的谱带。     

中小强度有氧运动后大鼠右心室肌蛋白质组差异表达的研究

目的:运用蛋白质组学的理论和方法来研究中小强度有氧运动对大鼠右心室肌蛋白质组的影响。方法:将20只SD雄性大鼠随机分为对照组和运动组(各1组,每组n=10)。采用中小强度运动负荷跑台训练建立运动实验模型,提取右心室肌组织全蛋白样品,采用双向凝胶电泳进行蛋白样品分离。运用ImageMaster 2D Platinum图像分析软件对2-DE胶进行分析,通过图像分析,选取运动后差异表达量≥5倍的蛋白点,用串联飞行时间质谱蛋白质仪(ULGRAFL-FLEX-TOF/TOF)进行质谱鉴定。结果:经过8周运动后,在2-DE图谱上运动组和对照组检测到的蛋白质点分别为(547±18)个和(530±14)个,匹配率分别为(71.34±3.25)%和(70.28±2.38)%。运动后共有58个蛋白质点发生了变化:表达量上调≥2倍的点有33个,下调≥2倍的点有25个,其中上调≥5倍的点有8个,下调≥5倍的点有8个。选择差异表达量≥5倍的蛋白质点为目标蛋白质进行质谱分析,共鉴定出了5个差异表达蛋白质点。结论:在长期中小强度有氧运动后,大鼠右室肌蛋白质组发生了明显的差异性表达,这些差异性表达的蛋白质点为进一步观察在运动应激状态下对心脏...     

Adjustments of serum lactate dehydrogenase isoenzymes and their significance in monitoring the treatment in patients with tubercular pyothorax

The adjustments and diagnostic significance of polyacrylamide gel electrophoretic (PAGE) profiles of lactate dehydrogenase isoenzymes (LDH: 1.1.1.27) was evaluated in the sera and pleural fluid of patients with tubercular pyothorax. Sera and pleural fluid samples were randomly collected from 72 and 18 patients respectively at two different timings; first, when patients were admitted to the Hospital and second, after an intensive phase of treatment. Sera of 20 healthy individuals served as control. Our results demonstrate significant differences in sera LDH (sLDH) and pleural fluid LDH (pfLDH) isoenzymes. In patients the order of LDH isoenzyme in sera and pleural fluid followed: LDH−5>−4>−2>−3>−1 and LDH-5>−4>−3>−2>−1 respectively. The ranking of activity levels in control was LDH−2>−1>−3>−5>−4. In the second phase of sampling from 31 patients, values of sLDH isoenzymes showed recovery and resembled profiles of controls. Therefore, the sLDH zymograms of patients can be used as the prognostic marker, since they tend to reach the normal level during recovery signifying the effect of chemotherapy in hospitalized patients. Moreover, according to the present findings on LDH-PAGE profiles, the levels of LDH-5 and-4 rise in pyothorax patients significantly (P<0.05). This elevation along with the rise in total LDH activity may, therefore, be used in the diagnosis and monitoring of tubercular pyothorax.     

单细胞组分分析的研究进展

单细胞内组分的分析和测定对疾病的治疗和药物筛选具有重要的意义。本文比较了两种单细胞组分分析的方法即毛细管电泳和微流控芯片,并叙述了微流控芯片在单细胞研究方面的进展。