用密度梯度离心法富集源于小鼠胚胎干细胞的运动神经元样细胞前体

目的:我们的目标是从源于胚胎体(EBs)的小鼠胚胎干细胞(mESCs)中分离运动神经元样细胞前体(MNLCPs),以用于发展针对运动神经元疾病的药物筛选试验和移植疗法.天然Shh蛋白(或Shh通路激动剂)和维甲酸诱导的胚胎体中,MNLCPs和未分化细胞的含量是不确定的.如果不把未分化的细胞从细胞培养中充分去除,其可能会干涉药物筛选试验或在移植后增生.我们开发了一种以密度梯度离心法为基础的富集MNLCPs的方法.方法:我们用Wichterle等人2008年改进的方法,将mESCs( HBG3:eGFP:HB9)扩大和分化.通过化学和酶学的无研磨处理,含有绿色荧光蛋白的MNLCPs和未分化细胞的胚胎体被小心轻轻地离解成单细胞.利用OptiprepTM8％～20％逐步梯度离心技术将MNLCPs回收.拥有绿色荧光蛋白的MNLCPs的含量由流式细胞仪检测.结果:我们的结果表明,在胚胎体形成前,mESCs在明胶包被的培养板上生长,其分化为MNLCPs的能力减少.比较mESCs在明胶,明胶与PMEFs,及PMEFs包被的培养板上的生长发现,mESCs在PMEFs包被的培养板上产生含绿色荧光蛋白的MNLCPs的得率为(54.1±11.0)％(x-±s;n=12),在明胶包被的培养板上的得率为(2.8±1.1)％(x-±s;n=9).用密度梯度离心法获得的含绿色荧光蛋白的MNLCPs的平均含量为(87.7±5.5)％(x-±s;n=3).结论:我们的数据表明,不使用细胞分选器,无研磨解离和密度梯度离心法也能用于富集具有高存活率的MNLCPs.有必要对MNLCPs在体外、体内和表型上进行进一步的生理学意义(如神经轴突的生长及形成神经肌肉接头的能力)上的鉴定.     

An efficient method of addressing ectopic beats: new insight into data preprocessing of heart rate variability analysis

Heart rate variability (HRV) analysis is affected by ectopic beats. An efficient method was proposed to deal with the ectopic beats. The method was based on trend correlation of the heart timing signal. Predictor of R-R interval (RRI) value at ectopic beat time was constructed by the weight calculation and the slope estimation of preceding normal RRI. The type of ectopic beat was detected and replaced by the predictor of RRI. The performance of the simulated signal after ectopic correction was tested by the standard value using power spectrum density (PSD) estimation, whereas the results of clinical data with ectopic beats were compared with the adjacent ectopic-free data. The result showed the frequency indexes after ectopy corrected had less error than other methods with the test of simulated signal and clinical data. It indicated our method could improve the PSD estimation in HRV analysis. The method had advantages of high accuracy and real time properties to recover the sinus node modulation.     

行人最大速度对行人流影响的研究

应用改进的格子气模型(即将行人的最大速度扩展到大于1)研究行人的最大速度对过道中单向和双向行人流的影响。模拟结果显示:最大速度为5时所对应基本图的临界密度跟实验值比较符合,说明该扩展是必要的;在一定范围内随着行人最大速度的增大道路的整体通行能力增强同时临界密度减小;双向行人流的时空斑图显示行人最大速度的增大加快了拥堵的形成过程(即缩短了行人从局部拥堵演化为完全拥堵的时间)。     

基于遗传算法的题库选题设计与实现

从遗传算法的应用入手,阐述了遗传算法的相关概念和执行流程,深入研究了遗传算法在题库选题系统中的应用,探讨了初始种群、适应度函数、选择算子、交叉算子、变异算子的设计方法,并给出了关键实现代码。遗传算法为题库选题提供了高效的解决方案,能够取得良好的选题效果。     

基于动态密度的空中交通复杂度计算方法研究

空中交通复杂度是指管制员所指挥空域的复杂程度。当航空器在空中的运行发生变化时,会产生大量的潜在冲突。本文基于成熟的空中动态密度模型,将诸多参数进行分类,并选出最能表征这一类的突出参数,建立新的复杂度计算模型。并参考管制员解决冲突时所产生的冲突负荷,分别从交通密度、交通进展、飞行流结构、交通混合四个方面进行修正,得出复杂度的计算公式。并利用雷达数据对某一空域进行测算,得出该空域的交通复杂度值。     

Childhood sexual abuse by representatives of the Roman Catholic Church: A prevalence estimate among the Dutch population

Estimates of the extent of childhood sexual abuse (CSA) within in the Roman Catholic Church (RCC) in the general population are difficult to find. The independent Commission of Inquiry into sexual abuse of minors in the RCC in the Netherlands collected population-based data to estimate its prevalence. A large random online population sample was surveyed using a two-phase stratified sampling procedure. In Phase 1, 34,267 subjects aged 40 years and older were screened for childhood exposure to sexual abuse by non-family members, a history of institutionalization and a Roman Catholic upbringing. In Phase 2, a stratified subset of 2,462 subjects was assessed to obtain more detailed target information about sexual abuse reports within the RCC. We employed multiple imputation for the estimation of RCC CSA in the original Phase 1 sample. The prevalence of non-familial CSA in general (14.0%) was higher among women (17.2%) than among men (10.6%). The prevalence of CSA within the Dutch RCC (1.7%) was higher among men (2.7%) than among women (0.7%). As expected, older subjects reported more often CSA in the RCC than their younger counterparts. Respondents who stayed for some time in RCC run institutions for education or child protection had a higher risk to report sexual abuse. Although sexual abuse of minors by representatives of the RCC was a structural problem during a period that the Church was highly influential in the Netherlands, the estimated prevalence of the phenomenon is only a fraction of the prevalence rate of non-familial CSA.     

电磁场动量流密度的应用

阐述了由电磁场动量流密度张量求电磁场合力的方法,举例说明对于同一个问题可以用多种积分限计算的物理原因．     

双运动原子与单模光场相互作用系统的原子布居数演化特性

运用全量子理论研究单模光场与双运动原子相互作用系统的原子布居演化规律,结果表明,原子布居数振荡的频率随原子运动速度和光子数的增大而增大,振幅随原子运动速度和光子数的增大而减小。     

健身气功·八段锦对中老年人血脂的影响

研究哈尔滨市健身气功·八段锦练习者75天锻炼前后血脂指标的影响,结果表明,习练健身气功·八段锦后,老年男、女的血清TC浓度显著低于锻炼前;老年男、女锻炼后的血清LDL浓度非常显著低于锻炼前;中年男、女锻炼后的血清吼浓度非常显著高于锻炼前.通过结果分析对健身气功·八段锦的健身效果进行客观评价,使"八段锦"这一养生锻炼的形式更科学、更合理,并进一步提高其健身效果,为改善人民的健康水平发挥更大作用,为全民健身服务,为社会的政治稳定服务.     

广州市城乡居民参加体育活动的情况暨体育人口调查

采用PPS和KISH标准化抽样方法，对广州市12个区／县的4800户(人)进行了入户调查。并运用社会科学分析软件包SPSSl1．0统计软件，在对所得数据进行统计处理后。全方位地描述分析了广州市社会体育人口的地理分布、各项特征、参加体育活动的主客观动因以及影响社会体育人口形成的主要因素，最后阐述了本次调查的几点启示。