基于考生水平表现标准评价的探索与实践——以2014年高考语文(天津卷)为例

论述了近年来天津市基于考生水平表现标准的高考评价的理论探索与实践。以2014年高考(天津卷)为例展现基于考生水平表现标准评价的具体应用和分析结果。笔者依据《考生水平表现标准》,从知识范畴和能力属别两个维度,对全市、不同区域全体及不同水平组的普通高中应届生考生在2014年高考语文试卷的作答表现进行分析。全市共有90.23%的考生达到基本水平及以上,且分别在语言知识和语言表达知识范畴及分析综合能力属别的作答表现最好。2014年不同区域考生的语文水平差距在缩小,趋于均衡。通过典型试题进一步分析了考生水平和教学质量中存在的共性问题,并提出了切实改变课堂教学方式,加强积累、注重落实,提高学生的思维品质等教学建议。     

基层公共图书馆员服务礼仪建设初探

目前，我国中西部地区的基层公共图书馆硬件条件普遍较差，加之图书馆员队伍整体素质不高，致使这些图书馆的读者利用率一直徘徊不前。要打破这种局面，图书馆员服务礼仪建设是一项重要的基础性工作。     

Cloning, expression, purification, and characterization of LC-1 ScFv with GFP tag

INTRODUCTION Because of its low molecular weight and ability to fluoresce independently (George, 1997), the new molecular tag, green fluorescent protein (GFP), has become more and more popular after Prasher et al.(1992) cloned its cDNA in 1992. There are many reports describing the co-expression of GFP and a specific antibody or cytokine gene, with the fusion protein expressing the fluorescent activity and bio-logical activity of the complement protein (Haraguchi et al., 1999; Mclean…     

"三个代表"思想与"三个有利于"标准

江泽民同志“三个代表”思想的提出,是在坚持“三个有利于”标准的基础上,为适应新的历史时期的党的工作而提出的新的目标要求,是对“三个有利于”标准的坚持和发展。     

耐力运动对高脂膳食诱导的脂肪肝小鼠UCP2与Mn-SOD表达及与脂肪肝关系的研究

目的:研究耐力运动对高脂膳食诱导的C57BL/6J脂肪肝小鼠肝脏UCP2及Mn-SOD表达与脂肪肝的关系.方法:以高脂膳食诱导的脂肪肝小鼠为模型,分析耐力运动20周后肝脏UCP2及Mn-SOD表达变化与肝脏脂肪变的关系.结果:肝脏UCP2与Mn-SOD在高脂膳食组(HS组)均显著升高;恢复正常膳食结合耐力运动20周组(RE组),Mn-SOD表达进一步升高,而UCP2表达显著降低.HS组形成明显的脂肪肝,恢复正常膳食加20周不运动组与恢复正常膳食结合20周耐力运动组脂肪肝得到明显改善,但RE组改善更为明显.结论:耐力运动可能以上调Mn-SOD表达而下调UCP2表达,达到既清除活性氧又减少肝脏线粒体ATP合成降低目的,从而保护肝脏免受过多活性氧和ATP耗尽的损伤.     

Considerations for protein intake in managing weight loss in athletes

Abstract

A large body of evidence now shows that higher protein intakes (2–3 times the protein Recommended Dietary Allowance (RDA) of 0.8 g/kg/d) during periods of energy restriction can enhance fat-free mass (FFM) preservation, particularly when combined with exercise. The mechanisms underpinning the FFM-sparing effect of higher protein diets remain to be fully elucidated but may relate to the maintenance of the anabolic sensitivity of skeletal muscle to protein ingestion. From a practical point of view, athletes aiming to reduce fat mass and preserve FFM should be advised to consume protein intakes in the range of ～1.8–2.7 g kg^?1 d^?1 (or ～2.3–3.1 g kg^?1 FFM) in combination with a moderate energy deficit (?500 kcal) and the performance of some form of resistance exercise. The target level of protein intake within this recommended range requires consideration of a number of case-specific factors including the athlete's body composition, habitual protein intake and broader nutrition goals. Athletes should focus on consuming high-quality protein sources, aiming to consume protein feedings evenly spaced throughout the day. Post-exercise consumption of 0.25–0.3 g protein meal^?1 from protein sources with high leucine content and rapid digestion kinetics (i.e. whey protein) is recommended to optimise exercise-induced muscle protein synthesis. When protein is consumed as part of a mixed macronutrient meal and/or before bed slightly higher protein doses may be optimal.     

放射性物位计在连续重整装置的应用

催化剂再生系统是连续重整装置反再部分的核心环节。在催化剂再生和循环的过程中需要准确检测催化剂的物位,为控制系统提供可靠参数。放射性物位计就是一种能准确检测设备中催化剂物位的仪表。本文介绍了放射性物位计的工作原理、配置方法和防护事项。同时,结合国产连续重整工艺,介绍了放射性物位计在连续重整中的设置和标定。     

Effect of microcystin-LR on protein phosphatase 2A and its function in human amniotic epithelial cells

Due to their toxicity, the increased distribution of microcystins (MCs) has become an important worldwide problem. MCs have been recognized as inhibitors of protein phosphatase 2A (PP2A) through their binding to the PP2A catalytic subunit. However, the exact mechanism of MC toxicity has not been elucidated, especially concerning the cellular response and its autoregulation. To further dissect the role of PP2A in MC-induced toxicity, the present study was undertaken to determine the response of PP2A in human amniotic epithelial (FL) cells treated with microcystin-LR (MCLR), one of the MC congeners. The results show that a low-dose treatment of MCLR in FL cells for 6 h induced an increase in PP2A activity, and a high-dose treatment of MCLR for 24 h decreased the activity of PP2A, as expected. The increased mRNA and protein levels of the PP2A C subunit may explain the increased activity of PP2A. Furthermore, MCLR altered microtubule post-translational modifications through PP2A. These results further clarify the underlying mechanism how MCLR affects PP2A and may be helpful for elucidating the complex toxicity of MCLR.     

中国女排竞技水平发展特征及成因研究(一)——初步发展阶段分析

以中国女排竞技水平发展历程为研究主题,运用文献资料、问卷调查、数理统计等方法对建国初中国女排竞技水平发展特征及主要影响因素进行分析,分析结果显示建国初中国女排竞技水平发展特征为起点低、进步快,技战术水平呈现两个高峰;其主要影响因素为政治环境、训练理论与方法、运动员、竞训管理、教练员和训练环境因素。     

游泳训练科学化水平的分析与展望

本文对我国游泳训练的规律以及训练方法等方面做了分析和归纳,从中找出可借鉴的成功游泳训练经验,对游泳科学训练的发展提出了建议,希望能为游泳科学训练提供参考依据。