人细胞周期蛋白D1在大肠杆菌中的可溶性表达与纯化

将人细胞周期蛋白D1基因克隆入原核表达载体pET-20b中获得重组质粒pET-20b-eyeD,经酶切鉴定正确后转化大肠杆菌BL21PlaysS后获得表达菌株．该菌株经IPTG诱导后表达的目的蛋白有部分分泌到培养基上清中,将培养基上清中的蛋白沉淀后用Ni^2＋螯合柱进行纯化,最后可得到纯度达到95％以上的目的蛋白．蛋白电泳显示纯化蛋白的分子量约为33KD,Westemblot分析表明,在电泳胶的相应分子量处出现特异性条带,说明已经成功表达和纯化了重组人细胞周期蛋白D1．     

基于层次网络的最大流问题研究

为了分层实现最大流问题,提出了一种应用DFS策略搜索增广路径,先求得层次网络阻塞流进而最终求得一个最大流的方法。     

督师导教 稳步提升我校教育教学水平

伴随着我校改制的顺利完成和新的发展任务出现,构建严谨的教学质量监控体系成了迫切的需要。我校成立教学督导组织机构,旨在通过督师导教,促进教学秩序的稳定和教学质量的整体提高。     

谈如何推动我国企业间的技术合作

在我国企业间进行的合作远不如西方国家广泛,深入,如何才能为企业创造一个有利于合作形成和发展的环境并保进我国企业间合作水平的提高呢,本以技术合作为例,提出了苦干条推动企业间技术合作的建议,这些建议大多也适用一地其它形式的合作。     

英国14-19岁普通教育考试制度与高校入学机制的关系研究

本文研究了英国14-19岁阶段普通教育的考试制度及其与高校入学机制的关系.分析了其以资格证书为基础的考试制度的特点、经验和问题,为我国普通高中考试制度改革和高校入学机制改革提供参照.     

蛋白质修正卡方分布函数

利用统计分析和数据挖掘的知识,给出了蛋白质残基原子与其他原子的接触距离和接触数的定义,并根据蛋白质的种类的不同,计算了接触距离的数学期望和标准差,得到血红蛋白、激素和肌蛋白残基的概率分布,构造出类蛋白质ASP残基接触数的修正卡方分布函数.     

北京市商业体育俱乐部消费者行为研究

运用文献研究法、问卷调查法对北京市高、中、低档次的商业体育俱乐部的消费者基本情况、体育消费水平、体育消费目的和动机及其满意度进行了分析。     

A ‘Multimodal Ecology of Text’ in Advanced Level English Language examinations? The incorporation of graphology and ‘embodied’ facsimile texts in unit test papers and their assessment since 2001

In this paper we compare the representation of texts in a sample of Advanced Level English Language examination papers set over the past decade in order to explore the changes in ‘what constitutes English and its assessment’. Prior to the year 2000, texts used in most A Level Language examination papers were usually typeset in a word processed format stripped of original font, layout, accompanying images, and colour, and devoid of the material marks of the texts' ‘history’ and materiality. From 2001, the texts for AQA B examination papers¹ have been reproduced in facsimile form to include the original graphology used. This shift in production in the materiality of embodiment has led to a corresponding shift in assessment in the codes of recognition of what constitutes English text, and what counts as English response. The sample of texts that we discuss present an ‘ecology of text’ and an ‘ecology of texts and literacy practices’ on a ‘continuum of multimodality’: from the heavily edited, word processed, linguistically circumscribed texts of the 1990s examination papers, through to the more visually‐dependent texts of 2004 with their manner of writing, typographic detail, colour, and sometimes even complete with creases and stains. Taking a multimodal approach to these texts, we discuss the implications of this change for what is being required of students analysing texts for examination assessment and more broadly for the subject ‘English’ ‐ ‘what English is’. We also consider how this shift problematizes the English work of students, teachers, examiners, and the institutions in which these agents operate.     

陕面佛坪县图书馆的现状与发展思路

图书馆事业是人类社会重要的组成部分，随着社会的发展、新农村建设的加快、科学技术的进步、社会分工的日趋合理与完善，社会对图书馆的需求越来越大。结合佛坪的实际情况，论述县级图书馆只有立足现实，着眼长远，多方着手，才能求得生存与发展。