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1.
INTRODUCTION Because of its low molecular weight and ability to fluoresce independently (George, 1997), the new molecular tag, green fluorescent protein (GFP), has become more and more popular after Prasher et al.(1992) cloned its cDNA in 1992. There are many reports describing the co-expression of GFP and a specific antibody or cytokine gene, with the fusion protein expressing the fluorescent activity and bio-logical activity of the complement protein (Haraguchi et al., 1999; Mclean… 相似文献
2.
J. I. Anetor F I Ajose T. S Akingbola 《Indian journal of clinical biochemistry : IJCB》2005,20(1):193-197
A 64-year-old security guard and longstanding known hypertensive presented with hypertensive heart disease (HHD), weight loss,
an enlarged prostate, and a spontaneously fractured rib. Malignancy of the prostate with possible metastases to the ribs was
strongly suspected. Biochemical profiling revealed a paraprotein. Other biochemical and hematological correlates that were
on hand before serum protein electrophoresis were rather atypical. Paraprotein studies by immunofixation revealed IgA myelomatosis.
Unlike previous reports from Caucasians there was normocalcaemia, normal protein level, microcytic hypochromia, low MCHC,
cholesterol level at the lower limit of the reference range and normal urea level (in the face of markedly raised creatinine
level). Nutritional modulation of the classical laboratory features of this disease may account for the fairly atypical laboratory
picture.
The need to appreciate the influence of nutritional status on the laboratory (especially biochemical) features of a disease
and thus interpretation of diagnostic tests appears of exceptional current importance, given the widening gap in socioeconomic
status and the level of poverty between the resource poor and developed countries from which the classical, clinical and laboratory
features of most diseases were first described.
This case report reminds of the need not only to recognize theoretically the impact of nutritional status on the laboratory
characteristics of a disease but of the practical application of the nutritional perspective in the interpretation of diagnostic
investigations, especially in nutritionally disadvantaged communities. 相似文献
3.
耐力运动对高脂膳食诱导的脂肪肝小鼠UCP2与Mn-SOD表达及与脂肪肝关系的研究 总被引:1,自引:0,他引:1
目的:研究耐力运动对高脂膳食诱导的C57BL/6J脂肪肝小鼠肝脏UCP2及Mn-SOD表达与脂肪肝的关系.方法:以高脂膳食诱导的脂肪肝小鼠为模型,分析耐力运动20周后肝脏UCP2及Mn-SOD表达变化与肝脏脂肪变的关系.结果:肝脏UCP2与Mn-SOD在高脂膳食组(HS组)均显著升高;恢复正常膳食结合耐力运动20周组(RE组),Mn-SOD表达进一步升高,而UCP2表达显著降低.HS组形成明显的脂肪肝,恢复正常膳食加20周不运动组与恢复正常膳食结合20周耐力运动组脂肪肝得到明显改善,但RE组改善更为明显.结论:耐力运动可能以上调Mn-SOD表达而下调UCP2表达,达到既清除活性氧又减少肝脏线粒体ATP合成降低目的,从而保护肝脏免受过多活性氧和ATP耗尽的损伤. 相似文献
4.
AbstractA large body of evidence now shows that higher protein intakes (2–3 times the protein Recommended Dietary Allowance (RDA) of 0.8 g/kg/d) during periods of energy restriction can enhance fat-free mass (FFM) preservation, particularly when combined with exercise. The mechanisms underpinning the FFM-sparing effect of higher protein diets remain to be fully elucidated but may relate to the maintenance of the anabolic sensitivity of skeletal muscle to protein ingestion. From a practical point of view, athletes aiming to reduce fat mass and preserve FFM should be advised to consume protein intakes in the range of ~1.8–2.7 g kg?1 d?1 (or ~2.3–3.1 g kg?1 FFM) in combination with a moderate energy deficit (?500 kcal) and the performance of some form of resistance exercise. The target level of protein intake within this recommended range requires consideration of a number of case-specific factors including the athlete's body composition, habitual protein intake and broader nutrition goals. Athletes should focus on consuming high-quality protein sources, aiming to consume protein feedings evenly spaced throughout the day. Post-exercise consumption of 0.25–0.3 g protein meal?1 from protein sources with high leucine content and rapid digestion kinetics (i.e. whey protein) is recommended to optimise exercise-induced muscle protein synthesis. When protein is consumed as part of a mixed macronutrient meal and/or before bed slightly higher protein doses may be optimal. 相似文献
5.
Due to their toxicity, the increased distribution of microcystins (MCs) has become an important worldwide problem. MCs have
been recognized as inhibitors of protein phosphatase 2A (PP2A) through their binding to the PP2A catalytic subunit. However,
the exact mechanism of MC toxicity has not been elucidated, especially concerning the cellular response and its autoregulation.
To further dissect the role of PP2A in MC-induced toxicity, the present study was undertaken to determine the response of
PP2A in human amniotic epithelial (FL) cells treated with microcystin-LR (MCLR), one of the MC congeners. The results show
that a low-dose treatment of MCLR in FL cells for 6 h induced an increase in PP2A activity, and a high-dose treatment of MCLR
for 24 h decreased the activity of PP2A, as expected. The increased mRNA and protein levels of the PP2A C subunit may explain
the increased activity of PP2A. Furthermore, MCLR altered microtubule post-translational modifications through PP2A. These
results further clarify the underlying mechanism how MCLR affects PP2A and may be helpful for elucidating the complex toxicity
of MCLR. 相似文献
6.
组织芯片技术及其在体育生物中应用展望 总被引:2,自引:0,他引:2
组织芯片是cDNA微阵列的基础上发明的一种特殊的生物芯片。本文介绍了组织芯片技术的制作原理、流程、发展历史、现状、特点和功能。并对组织芯片技术在运动选材、机能评定、运动营养等方面的应用前景进行了讨论,旨在促进组织芯片技术与体育生物学的进一步结合,推动体育生物科学的发展。 相似文献
7.
8.
Harleen Mangat Tajamul Hussain C Sundaram MP JS Anandaraj 《Indian journal of clinical biochemistry : IJCB》2002,17(2):74-79
Poly A RNA status and itsin vitro translation in a rabbit reticulolysate cell free system, were assessed in the skeletal muscle of young boys with Duchenne
muscular dystrophy. Compared to normals there is a significant 48% increase in poly A RNA content of dystrophic muscle and
its translatability was increased by 56% based on35S methionine incorporation into total protein systhesised. Immunoprecipitation of the translated products with monospecific
antibodies showed that there is a 2.6 fold and a 2 fold increase in m and μ calpains respectively. This underlines the importance
of both synthetic and degenerative activities in the early pathology in DMD muscle. 相似文献
9.
Aflatoxins are the most popular hepatotoxicants. Chronic exposure to aflatoxins leads to a wide variety of liver diseases, such as hepatocellular carcinoma. In this study, we analyzed the genome wide expression profiles of aflatoxin B1-induced rat hepatic epithelial cells. The expression of 325, 184 and 199 special genes was altered when exposed to 0.03, 0.1 and 0.2 μmol/L aflatoxin B1 respectively, and 239 genes were commonly expressed. After the functional analysis on these dose-special genes, we determined several key pathways related to hepatotoxicity, such as TGF-beta signaling pathway, tight junction, adherens junction, the regulation of actin cytoskeleton, Erb B signaling pathway, p53 signaling pathway, pathways in cancer and axon guidance. Common genes were mainly associated with focal adhesion, ECM-receptor interaction, and cell adhesion molecules. Gene ontology annotations showed a good concordance with these pathways. The quantitative real-time polymerase chain reaction(PCR) analysis of selected genes showed similar patterns in microarrays. The toxicogenomic study provides a better understanding of molecular mechanisms of aflatoxins. 相似文献
10.
Xu-fei TAN Feng CHEN Shan-shan WU Yu SHI Dong-cheng LIU Zhi CHEN 《Journal of Zhejiang University. Science. B》2010,(3)
Objective:To find new protein biomarkers for the detection and evaluation of liver injury and to analyze the relationship between such proteins and disease progression in concanavalin A (Con A)-induced hepatitis.Methods:Twenty-five mice were randomly divided into five groups:an untreated group,a control group injected with phosphate buffered saline (PBS),and groups with Con A-induced hepatitis evaluated at 1,3 and 6 h.Two-dimensional gel electrophoresis (2-DE) and mass spectrometry (MS) were used to identif... 相似文献