Nutritional quality, functional properties and anti-nutrient compositions of the larva of Cirina forda （Westwood） （Lepidoptera： Saturniidae）

INTRODUCTION Insects are the most successful group of animals constituting about 76% of known species of animals (Yoloye, 1988). Insects affect man either as destroy- ers of man’s valuable materials and crops or as sources of his nutrients. Goodman (1989) reported that chitin, an important insect component, can sig- nificantly reduce serum cholesterol, and serve as a haemostatic agent for tissue repairs and for acceler- ating healing of burns and wound. The cultural prac- tice of entomop…     

Expression, purification, and bioactivity of GST-fused v-Src from a bacterial expression system

v-Src is a non-receptor protein tyrosine kinase involved in many signal transduction pathways and closely related to the activation and development of cancers. We present here the expression, purification, and bioactivity of a GST （glutathione S-transferase）-fused v-Src from a bacterial expression system. Different culture conditions were examined in an isopropyl ,B-D-thiogalactopyranoside （IPTG）-regulated expression, and the fused protein was purified using GSH （glutathione） affinity chromatography. ELISA （enzyme-linked immunosorbent assay） was employed to determine the phosphorylation kinase activity of the GST-fused v-Src. This strategy seems to be more promising than the insect cell system or other eukaryotic systems employed in earlier Src expression.     

停流光谱在蛋白质动力学反应中应用的研究进展

介绍停流技术的原理,概述在线停流和圆二色谱、荧光光谱等仪器联用的现状,并讨论其在蛋白质与其它分子相互作用动力学过程及机理领域的应用。     

激素对驱蚊香草增殖过程中部分生理特性的影响

研究了BA和NAA处理组合对驱蚊香草增殖过程中糖和蛋白质含量的影响,结果表明: BA和NAA与BA互作对糖含量有极显著效应.而处理时间对蛋白质含量表现极显著差异.随着培养时间的延长蛋白质含量明显下降.说明继代周期不宜长,驱蚊香草增殖较适宜的培养基为:MS NAA0.4～0.8mg/L BA2.0～4.0mg/L.     

几种干果补充学龄前儿童蛋白质的比较分析

采用光谱法对几种干果中蛋白质的含量进行分析.光谱法的最大吸收波长为280nm,回归方程为A=0.5321C-0.0173,相关系数r=0.9999,线性范围为0～1.4973mg/mL.变异系数0.24％,回收率为99.32％～103.3％.实验结果表明,测定的干果样品中花生仁蛋白质含量最高为243mg/g.     

What we know about ST13, a co-factor of heat shock protein, or a tumor suppressor?

This article is to summarize the molecular and functional analysis of the gene “suppression of tumorigenicity 13” (ST13). ST13 is in fact the gene encoding Hsp70 interacting protein (Hip), a co-factor (co-chaperone) of the 70-kDa heat shock proteins (Hsc/Hsp70). By collaborating with other positive co-factors such as Hsp40 and the Hsp70-Hsp90 organizing protein (Hop), or competing with negative co-factors such as Bcl2-associated athanogen 1 (Bag1), Hip facilitates may facilitate the chaperone function of Hsc/Hsp70 in protein folding and repair, and in controlling the activity of regulatory proteins such as steroid receptors and regulators of proliferation or apoptosis. Although the nomenclature of ST13 implies a role in the suppression of tumorigenicity (ST), to date available experimental data are not sufficient to support its role in cancer development, except for the possible down-regulation of ST13 in gastric and colorectal cancers. Further investigation of this gene at the physiological level would benefit our understanding of diseases such as endocrinological disorders, cancer, and neurodegeneration commonly associated with protein misfolding.     

氨基酸紫外光谱的再测定

蛋白质含量分析在蛋白质化学、生物化学、食品科学、临床医学等领域的研究中起着重要的作用,紫外分光光度法测定蛋白质含量是生物化学实验中常开设的项目,对17种氨基酸在190~340 nm 波长的紫外吸收光谱进行了再测定,讨论了不同氨基酸紫外光谱的特点。并进一步利用紫外吸收法测定了牛血清白蛋白的含量,对其原理进行了验证和讨论。     

TMAs技术及其在体育生物科学领域中的应用展望

研究目的:探讨组织微阵列(tissuemicroarrays,TMAs或tissuechip)技术在体育生物科学领域中的应用前景。研究方法:采用历史回顾与前瞻性分析及逻辑推理相结合的方法,跟踪TMAs技术的发展及其在体育生物科学领域中的应用。研究结果与结论:TMAs技术是近年来基因芯片(DNA芯片)为代表的生物芯片技术的发展和延伸,它与细胞芯片、蛋白质芯片、抗体芯片一样,属于一种特殊的生物芯片技术。它将成百上千个不同个体或/和同一个体不同器官的组织标本排列在一张载玻片上所形成的组织微阵列生物芯片。TMAs是一种高通量、并行性、多样本研究mRNA、DNA、基因以及蛋白质定位、定性和相对定量的分析工具,在体育生物科学领域中具有广阔的应用前景。     

Efficacy of a Fish Protein Hydrolysate in Malnourished Children

Protein hydrolysates are good nutritional supplements as their bioactive ingredients can be easily absorbed and utilized for various metabolic activities. A fish protein hydrolysate (Amizate), prepared by a unique process of hydrolysis has the advantage of high di/tri peptide content (<10 kDa) along with essential and non essential amino acids, micronutrients and vitamins. The effect of Amizate on malnourished children (6–8 years, a total of 438) of Grade I and II (Gomez’s classification) with respect to immunoglobulins, CD4/CD8 ratios and hemoglobin was examined. Measurement of these parameters during the user trial study (at the beginning and the end after 4 months) indicated that the levels of the immunological parameters were not significantly altered by the Amizate treatment. The values of immunoglobulins and CD4/CD8 ratios of malnourished children (India) are in the normal range and are in accordance with the reported values of various ethnic groups.