拟南芥热激因子AtHsfAla在PET原核表达系统中的表达及纯化

以pErl30a为载体构建的表达拟南芥热激因子AtHsfAla的大肠杆菌EcoliM15（pET30a／His6一AtHsfAla）为实验材料,以IPTG诱导6XHis融合蛋白的表达并经过Ni2＋柱分离纯化AtHsfAla,再通过SDS—PAGE鉴定表达蛋白和纯化蛋白．结果显示,AtHsfAla蛋白在在PET原核表达系统中能够有效表达,并能通过亲和层析获得纯化的AtHsfAla蛋白．研究结果为揭示拟南芥热激因子AtHsfAla的作用机理奠定基础．     

犹太教纯洁化观念下的“共同体想象”再分析

犹太教基于"纯洁化"的生活形式的追求而指向的"共同体想象"就其本质而言具有世界性意义,这一点使其在后现代视野中具有了与基督教"上帝观"(后现代条件下)之内涵达成沟通的可能,即通过二者之间的"对话"具有达成关于未来生活设想的"共同意见"的可能性,而这可以为后现代社会下的人类整体未来走向提供有益的思想借鉴。     

A case of IgA multiple myeloma: Nutritional perspective in diagnostic testing

A 64-year-old security guard and longstanding known hypertensive presented with hypertensive heart disease (HHD), weight loss, an enlarged prostate, and a spontaneously fractured rib. Malignancy of the prostate with possible metastases to the ribs was strongly suspected. Biochemical profiling revealed a paraprotein. Other biochemical and hematological correlates that were on hand before serum protein electrophoresis were rather atypical. Paraprotein studies by immunofixation revealed IgA myelomatosis. Unlike previous reports from Caucasians there was normocalcaemia, normal protein level, microcytic hypochromia, low MCHC, cholesterol level at the lower limit of the reference range and normal urea level (in the face of markedly raised creatinine level). Nutritional modulation of the classical laboratory features of this disease may account for the fairly atypical laboratory picture. The need to appreciate the influence of nutritional status on the laboratory (especially biochemical) features of a disease and thus interpretation of diagnostic tests appears of exceptional current importance, given the widening gap in socioeconomic status and the level of poverty between the resource poor and developed countries from which the classical, clinical and laboratory features of most diseases were first described. This case report reminds of the need not only to recognize theoretically the impact of nutritional status on the laboratory characteristics of a disease but of the practical application of the nutritional perspective in the interpretation of diagnostic investigations, especially in nutritionally disadvantaged communities.     

寡聚核苷酸研究进展

对寡聚核苷酸近年来在化学及生物医学等领域的研究进展进行了比较全面的介绍,包括寡聚核苷酸片断与蛋白质、药物的相互作用以及反义寡聚核苷酸的研究进展及应用前景等。     

乳糖诱导Pfu DNA聚合酶基因的表达及酶的纯化

利用乳糖诱导重组大肠杆菌BL21(DE3)中Pfu DNA聚合酶基因的表达。对诱导菌株起始生长量、乳糖浓度和诱导持续时间进行了优化,尝试使用JK110弱阳离子交换柱和Sephadex G-75凝胶层析柱来分离纯化Pfu DNA聚合酶,并用PCR扩增实验检测酶活性。结果表明乳糖可以有效诱导Pfu酶的表达,JK110离子交换柱有较好的纯化效果,从500 mL培养液中可以得到3×105U的酶活,比活达22,200 U/mg,结果好于IPTG诱导表达。     

尚湖水环境变化趋势及对策

简述尚湖生态系统及环境容量及近几年来水环境变化趋势，提出严格控制和削减污染物排放总量，实施尚湖生态工程，提高水环境质量的对策措施。     

Cloning, expression, purification, and characterization of LC-1 ScFv with GFP tag

INTRODUCTION Because of its low molecular weight and ability to fluoresce independently (George, 1997), the new molecular tag, green fluorescent protein (GFP), has become more and more popular after Prasher et al.(1992) cloned its cDNA in 1992. There are many reports describing the co-expression of GFP and a specific antibody or cytokine gene, with the fusion protein expressing the fluorescent activity and bio-logical activity of the complement protein (Haraguchi et al., 1999; Mclean…     

含油有机废水的电化学除油褪色

采用电化学净化的方法对模拟废水进行除油褪色处理，分别从电解电压、电解时间等方面进行条件实验，结果表明：在室温下，该模拟废水电化学除油褪色的最佳条件是电压为6V，电解时间为20min．     

桑树DNJ提取、纯化和检测的研究进展

1-脱氧野尻霉素(1-deoxynojirimycin,DNJ)是存在于桑树中的一种多羟基哌啶生物碱,因其通过抑制α-葡萄糖苷酶活性,进而具有降血糖、抗病毒、抗肿瘤等生理活性和药理活性.DNJ广泛存在于桑树的各种组织中,不同桑树类型和品种中DNJ含量不同,采取的每一种提取、测定方法均具有选择性和特异性,只适用于某种类型或某种桑树品种样品的纯化和检测.同时,DNJ在桑树体内的含量较低,而且桑树体内其他次生代谢物质的干扰增加了DNJ提取和测定的难度.因此,目前文献中报道的DNJ提取、分离纯化和检测方法较多.针对已报道的DNJ的提取、分离纯化和检测方法,综述了近年来DNJ研究方面的最新进展,为更好利用和开发DNJ制品,为DNJ的工业化生产提供参考和借鉴.     

《哈克贝利·芬恩历险记》中水的意象

“水”是分析理解《哈克贝利·芬恩历险记》这部小说的重要元素,它构成了小说中的中心意象,既是母性的象征,又具有再生与复活的力量,反映了死亡与再生的主题。“水”不仅赋予小说某种神话般的寓意,还暗示了小说主人公情感的发展历程。