复杂网络在蛋白质折叠中的应用

简述了复杂网络的静态几何量及其机制模型,然后列举了复杂网络在蛋白质研究领域的应用进展．     

净化与美化:西方古代审美教育的理想

以古希腊为代表的西方古代审美教育强调通过对人心灵的美化和净化,实现特定人格的塑造和健康心灵的达成.以心灵净化和美化的美育实践以模仿为起点,在循序渐进的过程中寓教于乐,在个别性与普遍性的相融中得到人与社会的和谐统一.     

Improving the sensitivity of protein microarray by evanescent-field-induced fluorescence

To improve the sensitivity of protein microarray, a prism surface replaces the surface of the common microscope slide. The protein targets arrayed on the surface are hybridized and labelled by fluorescent probes. Evanescent excitation occurs when the convergent laser reaches the surface, and a photomultiplier tube detects the emitted fluorescent signal. A two-dimensional actuator scans the whole surface to achieve planar laser excitation and fluorescence collection. The penetration depth of the evanescent field into the protein targets is only some hundred nanometers and can be controlled by different incident angle of the laser beam, so the undesired background signals are reduced dramatically and the detection sensitivity is improved by a factor of 50 to 100 comparing to confocal excitation. This approach can detect low abundance analytes without signal amplification.     

发酵液中纳豆激酶盐析法分离的研究

就纳豆激酶(Nattokinase)的粗纯化方法进行了初步的研究．从初筛的10株纳豆杆菌(Bacillus natto)中筛选出一株高产纳豆激酶的菌株，用液体发酵法将其发酵，产生的纳豆激酶采用硫酸铵盐析法进行粗提纯．实验结果表明，硫酸铵盐在70％饱和度时提纯效果较好。     

Purification and some properties of a β-glucanase from a strain, Trichoderma reesei GXC

β-glucanase was purified from a solid-state culture of Trichoderma reesei on wheat bran in three steps which comprised ammonium sulfate precipitation, Sephadex G-100 chromatography, and DEAE-Sephadex A-50 chromatography. The molecular mass was determined to be 35.21 kilodaltons by sodium dodecyl sulfate-12.5% polyacrylamide gel electrophoresis. The β-glucanase at low pHs was more stable than that at high pHs, and optimum pH was 5.0. The optimum temperature was 60 ℃, and β-glucanase was relatively stable at below 40 ℃ for 60 min. The Km of the enzyme on β-glucan was 10.86 mg/ml, and the Vmax on β-glucan was 14286 μmol of glucose equivalents per mg of the pure enzyme per min. The β-glucanase activity was significantly inhibited by Fe3+ ions, and was reduced in the presence of Cu2+ ions, Mn2+ ions and Mg2+ ions at 5 mmol/L and 10 mmol/L, respectively. The β-glucanase activity was stimulated by Co2+ ions, Ca2+ ions, Zn2+ ions, and Fe2+ ions at 1 mmol/L and 5 mmol/L, respectively.     

洛伐他汀工艺现状及研究进展

洛伐他汀在防治心脑血管疾病药物中,因其作用机理明确,临床疗效显著,近年来其研究和应用受到世界各国医药学界和企业的普遍重视.从洛伐他汀的发现出发,对国内外洛伐他汀的工艺现状及研究进展做了全面的综述.     

粗硫酸铜提纯实验中增大硫酸铜结晶颗粒的方法

向过饱和溶液中加入晶种以增大结晶颗粒是化工单元操作———结晶中最常用的方法 将该方法引入化学实验可达到实验过程误差减小、提高学生兴趣的效果     

能量和蛋白质对母猪繁殖性能的影响

本文从母猪营养需要的角度,综述了妊娠和哺乳母猪的能量和氨基酸的需要,及对改善母猪繁殖性能的重要意义。     

蛇足石杉生物碱的提取与测定

研究蛇足石杉生物碱的提取工艺.整个工艺是：用酸浸提、微波加热、蒸馏、萃取、反萃取、脱色等分离技术从蛇足石杉中提取石杉碱，最后定性定量检测.结果表明：（1）微波加热处理可提高石杉碱的提取率；（2）萃取和反萃取相结合，使用旋转蒸发仪蒸馏有助于分离石杉碱；（3）用碘化铋钾试液对提取的生物碱进行饱和滴定，然后烘干称量，再根据化学反应式来计算生物碱含量，是一种较好的近似测定方法；（4）根据标准样品的直线回归方程，利用紫外分析仪测定石杉碱甲含量是一种经济高效的方法；（5）洪江市塘湾镇的蛇足石杉的石杉碱甲的含量为0.085‰，粗提物中石杉碱甲的百分含量为26.1%.     

20世纪40年代与"纯诗"的综合化

“纯诗”的综合发展是中国新诗发展吏的一条主线。这种传统从20世纪20年代“纯诗”首倡之时即已建立,到40年代表现得更加自觉和明显。以袁可嘉、唐湜为代表的“中国新诗”派明确强调建立平衡的诗歌美学特征,在诗歌主题内容方面采取宽容综合的态度,试图建立纯诗的“高度综合的性质”和“现实、象征、玄学的新的综合传统”,走出了一条更具民族性和现实性的“纯诗化”道路。