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141.
Xing-wei Xiang Rui Yang Lin Chen Xiao-long Hu Shao-fang Yu Cui-ping Cao Xiao-feng Wu 《Journal of Zhejiang University. Science. B》2012,13(2):111-117
In the late phase of Bombyx mori nucleopolyhedrovirus (BmNPV) infection, a large amount of polyhedra appear in the infected cell nucleolus, these polyhedra
being dense protein crystals protecting the incorporated virions from the harsh environment. To investigate whether the foreign
protein could be immobilized into the polyhedra of BmNPV, two recombinant baculoviruses were generated by a novel BmNPV polyhedrin-plus
(polh+) Bac-to-Bac system, designated as vBmBac(polh+)-enhanced green fluorescent protein (EGFP) and vBmBac(polh+)-LacZ, which can express the polyhedrin and foreign protein simultaneously. Light microscopy analysis showed that all viruses
produced polyhedra of normal appearance. Green fluorescence can be apparently detected on the surface of the vBmBac(polh+)-EGFP polyhedra, but not the BmNPV polyhedra. Fluorescence analysis and anti-desiccation testing confirmed that EGFP was
embedded in the polyhedra. As expected, the vBmBac(polh+)-LacZ polyhedra contained an amount of LacZ and had a higher β-galactosidase activity. Sodium dodecyl sulfate polyacrylamide
gel electrophoresis (SDS-PAGE) and Western blotting were also performed to verify if the foreign proteins were immobilized
into polyhedra. This study provides a new inspiration for efficient preservation of useful proteins and development of new
pesticides with toxic proteins. 相似文献
142.
143.
Heuristic algorithm for off-lattice protein folding problem 总被引:1,自引:0,他引:1
Enlightened by the law of interactions among objects in the physical world, we propose a heuristic algorithm for solving the three-dimensional (3D) off-lattice protein folding problem. Based on a physical model, the problem is converted from a nonlinear constraint-satisfied problem to an unconstrained optimization problem which can be solved by the well-known gradient method. To improve the efficiency of our algorithm, a strategy was introduced to generate initial configuration. Computational results showed that this algorithm could find states with lower energy than previously proposed ground states obtained by nPERM algorithm for all chains with length ranging from 13 to 55. 相似文献
144.
利用RT-PCR技术从日本对虾中克隆到β-actin基因的cDNA,通过双酶切将其克隆到原核表达载体pGEX-4T-2中,转化大肠杆菌E.coli BL21感受态细胞,获得阳性克隆.4℃下IPTG诱导表达,Glutathione Sepharose 4B纯化后获得高纯度的β-actin蛋白.① 相似文献
145.
Xu-fei Tan Feng Chen Shan-shan Wu Yu Shi Dong-cheng Liu Zhi Chen 《Journal of Zhejiang University. Science. B》2010,11(3):221-226
Objective: To find new protein biomarkers for the detection and evaluation of liver injury and to analyze the relationship between such proteins and disease progression in concanavalin A (Con A)-induced hepatitis. Methods: Twenty-five mice were randomly divided into five groups: an untreated group, a control group injected with phosphate buffered saline (PBS), and groups with Con A-induced hepatitis evaluated at 1, 3 and 6 h. Two-dimensional gel elec-trophoresis (2-DE) and mass spectrometry (MS) were used to identify differences in protein expression among groups. Quantitative real-time polymerase chain reaction (qRT-PCR) was performed to verify the results. Results: In mice with Con A-induced hepatitis, expression levels of four proteins were increased: RIKEN, fructose bisphosphatase 1 (fbp1), ketohexokinase (khk), and Chain A of class pi glutathione S-transferase. Changes in fbp1 and khk were confirmed by qRT-PCR. Conclusion: Levels of two proteins, fbp1 and khk, are cleady up-regulated in mice with Con A-induced hepatitis. 相似文献
146.
为了提高教学效果,进行了生物物质分离与纯化课程教学模式的改革,整合教学内容、进行双师素质教学队伍建设、完善校内外实训基地、将成本核算及清洁生产等的理念贯穿到教学过程中,并建立有利于综合职业能力和素质培养的评价体系,使培养的学生具备较高的综合职业能力。 相似文献
147.
通过文献检索方法对腺苷酸活化蛋白激酶参与运动引起骨骼肌适应的基因表达调节机制进行综述.从腺苷酸活化蛋白激酶相关生物知识、运动调节腺苷酸活化蛋白激酶激活和腺苷酸活化蛋白激酶参与运动训练与基因表达的可能作用三个方面进行探讨,为运动训练提供参考. 相似文献
148.
149.
水生植物在水污染治理中的应用研究进展 总被引:9,自引:0,他引:9
阐述了水生植物治理水污染的研究进展情况、净化机理和效果.提出了水生植物在污水处理中需要关注的问题,并展望了该技术的发展前景. 相似文献
150.
Since preparation and solubilization of protein samples are crucial factors in proteome research, the authors established
a sequential extraction technique to prepare protein samples from the body wall of the 5th instar larvae of silkworm,Bombyx mori. Two kinds of protein samples were obtained from the body wall using the method. Between the two types of samples only about
15% proteins were identical; the majority were different, indicating that more species of proteins could be obtained with
the sequential extraction method; which will be useful for preparation of protein samples for proteome study. 相似文献