利用SPA提取鼠IgG制备兔抗鼠IgG血清的尝试

本文首次用 SPA 提取鼠 IgG,制备兔抗鼠 IgG 血清,效价高,纯度好,方法简便,可靠。     

裸子植物的生化系统学(四)——杉科植物的种子蛋白和针叶过氧化物酶

用SDS聚丙烯酰胺凝胶电泳分析了杉科全部10属共13种植物的种子蛋白多肽。它们     都有分子量为32、24和10K(千道尔顿)的多肽。每个分类群一般只有2至4个主要多肽，构     成种子蛋白总含量的一半;而且其分子量都分布在24—26K及29—36K两个区域内。  因此，     杉科植物种子蛋白谱与红豆杉科、三尖杉科相似，而不同于松科。属内不同种之间，种子蛋白     组成和针叶过氧化物酶电泳谱都没有看到显著差别。根据这两种蛋白质资料，表明杉科各属除红杉、巨杉、落羽杉相互很接近外，其他各属间关系都比较远。但是还不能说杉科可以分立几个科。     

芥菜中PP2A的生物信息学初步分析

以芥菜(Brassica juncea)中具有抗逆作用的植物蛋白磷酸酶PP2A为例,对其进行了生物信息学的初步分析。预测了PP2A的一级结构,二级结构及其基本性质。为生物信息学在研究抗逆蛋白中的应用提供了方法参考,为进一步研究PP2A在抗逆生理中的功能提供了有用的理论依据。     

对虾白斑综合症病毒囊膜蛋白Vp28基因的克隆、表达及蛋白纯化

以对虾白斑病毒DNA为模板扩增出Vp28基因,经限制性内切酶（SmaI,Not I）酶切后按正确的读码框顺序插入到pGEX-4T-2表达载体上,重组质粒转化大肠杆菌,经菌落PCR和质粒双酶切鉴定、序列测定确认,证实成功地构建了Vp28基因原核表达载体．转化菌经IPTG诱导表达,SDS-PAGE显示有与预期大小约52kD相吻合的融合蛋白带,18℃诱导24h后获得了可溶性表达的目的蛋白,采用Glutathione Sepharose 4B亲和层析对重组蛋白进行纯化,获得了纯度很高的目的蛋白．     

高渗盐水抗休克作用的淋巴机制

本实验采用肠淋巴管引流术,在失血性休克大鼠模型的基础上,观察高渗盐水治疗失血性休克过程中肠淋巴流量及其蛋白含量的变化,旨在探讨高渗盐水对失血性休克大鼠治疗的淋巴机制。结果表明,休克大鼠输入高渗盐水或生理盐水后,两组大鼠的血压、肠淋巴流量及其蛋白输出量均比休克期明显升高,高渗盐水治疗组显著高于生理盐水对照组(P<0.01),而且治疗组大鼠肠淋巴流量及其蛋白输出量远远高于休克前水平(P<0.01)。结果提示,高渗盐水的抗休克机制可能与其恢复休克大鼠的淋巴流量及其蛋白输出量、改善休克时肠淋巴循环障碍有关。     

人工湿地技术在我国北方地区的试验研究

本研究在邯郸构建了二级串联人工湿地系统,进行污水净化试验．试验结果表明,构建的人工湿地系统对污染物去除效果良好,出水水质较稳定,系统对COD、TN、TP的去除率分别在25％～65％、29％-94％、30％-80％之间．在相同的水力负荷下,植物生长状况良好的人工湿地污水处理效果明显高于植物生长状况差的人工湿地．潜流人工湿地子系统对污水的净化效果和稳定性明显好于表层人工湿地系统．人工湿地处理污水受温度和季节影响较大,在温度较高的夏季去除率远远高于温度较低的冬季．     

黄粉虫体内乙酰胆碱酯酶（AChE）的分布和纯化方法

文章研究了黄粉虫乙酰胆碱酯酶（AChE）的体躯分布和纯化方法技术。结果表明：黄粉虫幼虫和成虫体内乙酰胆碱酯酶活性均集中在头部和胸部,腹部的乙酰胆碱酯酶活性显著低于头部和胸部。成虫体内乙酰胆碱酯酶活性明显高于幼虫。通过Sepharose4B和SephadexG-200纯化后,AChE的最高纯化倍数是3.86和18.84倍。     

串联亲和纯化技术筛选hCLP46 的相互作用蛋白

hCLP46(human CAP10-like protein46)是从MDS-AML患者的CD34⁺干细胞cDNA文库中筛选出的基因.我们利用串联亲和纯化技术来筛选与hCLP46有相互作用的蛋白.通过体内交联-甘氨酸洗脱策略,检测到7条有差异的蛋白带,经液相色谱-质谱联用鉴定,得到了CNX和PDI等一系列内质网伴侣蛋白.所以hCLP46可能是一个糖蛋白,其成熟过程利用了BiP/Grp94 和CNX/CRT 2套伴侣蛋白系统.     

Ginsenosides stimulated the proliferation of mouse spermatogonia involving activation of protein kinase C

The effect of ginsenosides on proliferation of type A spermatogonia was investigated in 7-day-old mice.Spermatogonia were characterized by c-kit expression and cell proliferation was assessed by immunocytochemical demonstration of proliferating cell nuclear antigen (PCNA).After 72-h culture,Sertoli cells formed a confluent monolayer to which numerous spermatogonial colonies attached.Spermatogonia were positive for c-kit staining and showed high proliferating activity by PCNA expression.Ginsenosides (1.0～10 μg/ml) significantly stimulated proliferation of spermatogonia.Activation of protein kinase C (PKC) elicited proliferation of spermatogonia at 10-8 to 107 mol/L and the PKC inhibitor H7 inhibited this effect.Likewise,ginsenosides-stimulated spermatogonial proliferation was suppressed by combined treatment of H7.These results indicate that the proliferating effect ofginsenosides on mouse type A spermatogonia might be mediated by a mechanism involving the PKC signal transduction pathway.     

Post-exercise ingestion of different amounts of protein affects plasma insulin concentration in humans

Abstract

The synergistic stimulating effect of combined intake of carbohydrate and protein on plasma insulin concentration has been reported previously. However, it remains unclear whether the amount of protein ingested after exercise affects the concentrations of plasma insulin and amino acids. This study of trained men compared the effects of post-exercise co-ingestion of carbohydrate plus different amounts of whey protein hydrolysates (WPHs) with carbohydrate alone on (1) blood biochemical parameters of carbohydrate metabolism during the post-exercise phase, and (2) endurance performance. Eight trained men exercised continuously for 70 min. Immediately after exercise and 30, 60, 90, and 120 min later, the participants received supplements containing: (1) 17.5 g carbohydrate, (2) 3.0 g WPHs and 17.5 g carbohydrate (L-WPH), or (3) 8.0 g WPHs and 17.5 g carbohydrate (H-WPH). After a 2-h recovery period, the participants performed an endurance performance test. The concentrations of blood glucose were lower and plasma insulin significantly higher in the H-WPH trial compared with the carbohydrate trial. The concentrations of plasma amino acids were increased in a dose-dependent manner following ingestion of different amounts of WPHs with carbohydrate. Endurance performance was not significantly different between the three trials. Co-ingestion of carbohydrate and H-WPH was more effective than ingestion of carbohydrate alone for stimulating insulin secretion and increasing the availability of plasma amino acids. These results suggest that plasma concentrations of amino acids during the recovery period are determined by the amount of dietary protein ingested, and that it is necessary to increase the concentration of plasma amino acids above a certain level to stimulate insulin secretion.