微量蛋白质的光散射测定

提出了测定微量蛋白质的新方法，蛋白质，包括牛血清白蛋白（BSA），人血清白蛋白（HSA），牛血红蛋白（HB）能在PH4．3的酸性介质中与桑色素结合，使桑色素的光散射信号提高，在最佳实验条件下，BSA浓度在0．5－0．8ug/ml范围内呈线性关系，将这一方面用于合成样品中微量蛋白质的测定，结果满意。     

气浮技术及其在循环水养鱼系统中的应用展望

随着水产养殖业的大力发展,气浮分离技术在养殖水体净化中的应用也日益广泛。本文介绍了气浮分离技术的原理、在工业废水处理中的应用及养鱼循环水体净化的研究现状、进展和应用前景。     

Dyslipidemia in pregnancy may contribute to increased risk of neural tube defects -a pilot study in north Indian population

Neural tube defects are congenital structural abnormalities of the brain and vertebral column resulting from improper or non-timely closure of the neural tube. Prevalence of neural tube defects is reported to be higher among women with diabetes mellitus and obesity. This study was designed to investigate the relation between the presence of dyslipidemia in antenatal patients and the risk of fetal neural tube defects. The present study was an observational, cross-sectional study involving 129 pregnant women in 16 to 18 weeks gestation period. Of these, 80 women had normal pregnancies and 49 were clinically high-risk cases for neural tube defects. Fasting blood samples were analyzed for blood sugar and lipid profile by enzymatic assay and alpha-fetoprotein levels using Enzyme Immunoassay. Alpha-fetoprotein (AFP) values were converted to Multiples of Median (MoM) appropriate for the gestational age. Based on AFP values, women were labeled as screen negative (AFP <2 MoM, n= 102) and screen positive (AFP > 2 MoM, n =27). Screen positive women were further evaluated by ultrasound and 21 women were found to carry a neural tube defects positive pregnancy. Statistical analysis was done on SPSS software. Body weight of the women showed a significant positive correlation with serum triglycerides, plasma sugar and AFP MoM values. A significant difference was observed in serum cholesterol levels (p= 0.038), triglycerides (p=0.001) and plasma sugar levels (p=0.002) between normal women and those with neural tube defects positive pregnancy. The Odds ratio for neural tube defects risk in dyslipidemic cases was 24.23 (CI 4.73 − 148.60) with a relative risk of 12.12. Dyslipidemia especially hypertriglyceridemia was found to be significantly associated with fetal neural tube defects.     

指示剂有机试剂铬天青S的纯化研究

报告了铬天青S的硅胶干柱层析法的纯化方法,探讨了固定相、淋洗液、洗脱剂对纯化产物的影响,确定了以层析硅胶为固定相,以正丁醇：吡啶：氨水=2：1：1为淋洗剂,以蒸馏水为洗脱剂的纯化条件,经过红外光谱和液相色谱表证,使用本方法可获得纯度大于98.59%的产品,结果令人满意。     

生物信息学中的马尔可夫模型

随着人类基因组研究的不断发展,在世界各个（Genbank,SwissPort等）数据库中积累了大量蛋白质、核酸序列数据,如何分析和处理这些数据以获得更多的信息成为科学家们关心的核心问题,并由此产生了生物信息学。文章介绍了在生物信息学研究中常见的几种用于数据库搜索、序列比较、建立蛋白质模型、发现新基因等研究的马尔可夫模型。     

家庭:审美教育的摇篮与基地

家庭是美育的摇篮与基地。通过美化家庭生活环境、人际关系与业余生活实施家庭美育     

通过对单原子的局域测量实现纠缠纯化

从两对原子的纠缠态出发 ,通过对单原子的局域测量 ,实现了纠缠的纯化 该方案不同于一般的通过联合的Bell基测量而实现纠缠纯化的过程 在此方案里 ,纯化包含两方面的含义 ,即四原子的纠缠态纯化为两原子纠缠态以及部分两原子纠缠态纯化为最大纠缠态     

肝素酶的原核表达与表达条件优化

利用PCR技术从肝素黄杆菌克隆到肝素酶HepI基因,通过双酶切将其克隆到原核表达载体pGEX-4T-2中,转化大肠杆菌E．coliBL21感受态细胞,获得基因工程重组菌．12℃下IPTG诱导表达12h,Glutathione Sepharose 4B纯化后获得较高纯度的HepI酶蛋白．     

论高校网络思想道德环境的净化和建设

上网是大学生学习生活的重要内容,大量的良莠混杂的网络信息,对大学生的道德观念及价值判断造成了深刻影响,其消极方面集中表现为道德主体性的失落、道德失范和道德心理失衡。加强高校网络思想道德环境的净化和建设工作刻不容缓,这就要求净化校园的网络环境,保证网络信息健康文明的内容格调;教育学生自觉遵守网络法律规范,做文明网民‘鼓励和支持学校主管部门及“两课”教师创建专门网站,开辟高校网络思想教育新阵地。     

Poly A RNA status and expression of milli (m) and micro (μ) calpains in skeletal muscle of duchenne muscular dystrophy patients

Poly A RNA status and itsin vitro translation in a rabbit reticulolysate cell free system, were assessed in the skeletal muscle of young boys with Duchenne muscular dystrophy. Compared to normals there is a significant 48% increase in poly A RNA content of dystrophic muscle and its translatability was increased by 56% based on³⁵S methionine incorporation into total protein systhesised. Immunoprecipitation of the translated products with monospecific antibodies showed that there is a 2.6 fold and a 2 fold increase in m and μ calpains respectively. This underlines the importance of both synthetic and degenerative activities in the early pathology in DMD muscle.