全文获取类型
收费全文 | 306篇 |
免费 | 1篇 |
国内免费 | 7篇 |
专业分类
教育 | 230篇 |
科学研究 | 55篇 |
体育 | 8篇 |
综合类 | 18篇 |
文化理论 | 1篇 |
信息传播 | 2篇 |
出版年
2021年 | 7篇 |
2020年 | 2篇 |
2019年 | 3篇 |
2017年 | 2篇 |
2016年 | 2篇 |
2015年 | 6篇 |
2014年 | 18篇 |
2013年 | 13篇 |
2012年 | 25篇 |
2011年 | 22篇 |
2010年 | 13篇 |
2009年 | 20篇 |
2008年 | 20篇 |
2007年 | 15篇 |
2006年 | 26篇 |
2005年 | 29篇 |
2004年 | 18篇 |
2003年 | 14篇 |
2002年 | 19篇 |
2001年 | 9篇 |
2000年 | 6篇 |
1999年 | 8篇 |
1998年 | 3篇 |
1997年 | 3篇 |
1996年 | 1篇 |
1995年 | 1篇 |
1994年 | 1篇 |
1993年 | 1篇 |
1992年 | 3篇 |
1990年 | 2篇 |
1988年 | 1篇 |
1986年 | 1篇 |
排序方式: 共有314条查询结果,搜索用时 927 毫秒
81.
KIM Chong-Min WON Chung-In RYU Joonghyun CHO Cheol-Hyung BHAK Jonghwa KIM Deok-Soo 《浙江大学学报(A卷英文版)》2006,7(9):1492-1499
INTRODUCTION Being the bio era, a significant research effort has been devoted to the study of proteins since they are related to the critical body functions. There have been many studies on proteins, with the voluminous related data being currently available in various pub- lic repositories including the Protein Data Bank (PDB) (CATH, 2006; DALI, 2006; MSD, 2006; RCSB PDB, 2006; SCOP Database, 2006). Given the atomic complexes of proteins, ana- lyzing interactions between them… 相似文献
82.
Nuala Gregory 《Educational Philosophy and Theory》2017,49(13):1236-1245
AbstractThis article divides into two roughly equal parts, both of which aim to address the act rather than the art of drawing. The second part focuses on a theoretical discussion of drawing. The first bears on a number of themes including the role of drawing in colonial history, drawing and data collection, and drawing and memory. It begins by describing an episode that unfolded as an encounter between two worlds, and two ages—an episode whose meaning and effects are still controversial today. 相似文献
83.
郁达夫小说中的"忏悔情绪" 总被引:1,自引:0,他引:1
于宁志 《连云港师范高等专科学校学报》2002,(3):20-23
现代文学受基督教的影响 ,许多作家的作品中存在着强烈的忏悔意识。郁达夫的忏悔意识不是纯粹宗教意义上的忏悔 ,它不是发端于原罪 ,而是指向具体的个人生活。同郁达夫的小说风格一样 ,他的忏悔带有浓重的感伤色彩 ,忏悔情绪与真率、清新的抒情风格相联系 ,还体现着作家的创作意图。郁达夫在小说中不断地忏悔 ,自我谴责 ,既与充满矛盾的动荡时代有关 ,又与作家纤敏的情感、柔弱的性格和自我表现的坦率的气质有着密切联系 ,还受到基督教文化影响等因素的制约。 相似文献
84.
β-glucanase was purified from a solid-state culture ofTrichoderma reesei on wheat bran in three steps which comprised ammonium sulfate precipitation, Sephadex G-100 chromatography, and DEAE-Sephadex
A-50 chromatography. The molecular mass was determined to be 35.21 kilodaltons by sodium dodecyl sulfate-12.5% polyacrylamide
gel electrophoresis. The β-glucanase at low pHs was more stable than that at high pHs, and optimum pH was 5.0. The optimum
temperature was 60°C, and β-glucanase was relatively stable at below 40°C for 60 min. TheK
m of the enzyme on β-glucan was 10.86 mg/ml, and theV
max on β-glucan was 14286 μmol of glucose equivalents per mg of the pure enzyme per min. The β-glucanase activity was significantly
inhibited by Fe3+ ions, and was reduced in the presence of Cu2+ ions, Mn2+ ions and Mg2+ ions at 5 mmol/L and 10 mmol/L, respectively. The β-glucanase activity was stimulated by Co2+ ions, Ca2+ ions, Zn2+ ions, and Fe2+ ions at 1 mmol/L and 5 mmol/L, respectively.
Project supported by Foundation for University Key Teacher of the State Ministry of Education, the National Natural Science
Foundation of China (No. 30000118) and Zhejiang Provincial Natural Science Foundation of China (No. 399409). 相似文献
85.
作为一种优秀的杀菌剂 ,2 ,4 ,4′ 三氯 2′ 羟基二苯醚在 2 0世纪 70年代投入使用 .本文制备了 3种羟基二苯醚化合物 ,确定了它们的液相色谱分离条件 .对三氯 2′ 羟基二苯醚的纯化条件进行了研究 ,发现活性炭、膨润土及三氧化二铝等脱色剂对粗产品中各个组分的脱色作用各不相同 :2 ,4 ,4′ 三氯 2′ 羟基二苯醚可以被活性炭吸附 ,产品中的有色杂质可以被氧化铝吸附 ,产品中无色杂质则可被膨润土吸附 相似文献
86.
从土壤样品中提取和纯化微生物DNA方法研究进展 总被引:3,自引:0,他引:3
自然界存在的微生物只有一少部分能够通过标准纯培养方法获得,而绝大多数是难培养和不能培养的微生物,近年出现了一些新的直接从环境基质中提取微生物DNA的方法,用来研究环境中微生物多样性。对目前用于提取和纯化环境样品中微生物DNA的研究进展进行了综述. 相似文献
87.
以葛根异黄酮含量为44.2%的葛根粗提物为原料,通过对聚酰胺(13~40目)、AB-8、HP-20 三种树脂对葛根异黄酮静态吸附与解吸的比较,在筛选出一种具有较高吸附率与解吸率的树脂的基础上,系统优化了此种树脂柱分离纯化葛根异黄酮的技术参数.结果表明,静态吸附与解吸时,HP-20树脂最适合于葛根异黄酮分离纯化,吸附率、解吸率分别达57.53%、37.49%.HP-20树脂柱分离纯化葛根异黄酮的最优技术参数为:50%乙醇洗脱,流速0.9BV/h,3BV的洗脱液.在此工艺条件下,葛根异黄酮的洗脱率、纯度和得率分别达53.67%、55.78%和44.70%. 相似文献
88.
开发污染治理技术是当前国内外水处理领域的研究热点。磁场、超声波单独对污水处理的研究已经较多。首先分别介绍了磁分离和超声波水处理的原理,总结了各自的影响因素,然后探讨了两者强相互作用下对污水的效应,提出了处理污水的新方法,具有广阔的应用前景。 相似文献
89.
Pankaj Abrol Ashok Verma H. S. Hooda 《Indian journal of clinical biochemistry : IJCB》2001,16(2):221-223
Thyroid hormonal status was measured in 80 malnourished children of different grades (I–IV) of protein energy malnutrition
(PEM). Serum levels of tri-iodo thyronine (T3), thyroxine (T4) and thyroid stimulating hormone (TSH) were measured by radioimmunoassay. The results were compared with 20 healthy, age
and sex matched controls. Levels of T3 and T4 were significantly low in PEM cases whereas TSH levels were similar in PEM cases when compared to controls. 相似文献
90.
A 21-day laboratory incubation experiment was conducted to investigate the impact of pesticides (insecticide, herbicide, fungicide)
on paddy field soil health under controlled moisture (flooded soil) and temperature (25°C) environment. The electron transport
system (ETS)/Dehydrogenase activity showed negative correlation with pesticides concentrations, decreased with increase of
pesticide concentration. The higher doses (5 to 10 times field rates) of pesticides significantly inhibited ETS activity,
while lower rates failed to produce any significant reducing effect on the control. The toxicity of pesticides in decreasing
the ETS activity was in the order: insecticide > fungicide > herbicide, irrespective of their rates of application. The pesticides
increased the soil phenol content, which increased with increasing concentration of agrochemicals. The pesticide application
did not produce any significant change in soil protein content. The response of biomass phospholipid content was nearly similar
to that of ETS activity. The phospholipid content decreased with the addition of pesticides in the order insecticide > fungicide
> herbicide and the toxicity was in the order: 10 FR (field rate) > 5 FR > 1.0 FR > 0.5 FR > control.
Project (No. G1999011809) supported by the National Key Basic Research Foundation (973) of China
Ph. D. student at Zhejiang University, from Ministry of Agriculture (Punjab), Government of Pakistan 相似文献