长期运动训练对运动性低血色素大鼠组织转铁蛋白及转铁蛋白受体基因表达的影响

了解发生运动性低血色素时转铁蛋白及转铁蛋白受体基因表达的变化对深入研究运动性贫血的发生机制具有重要的意义.实验结果表明:长期运动训练导致运动性低血色素大鼠肌肉组织中转铁蛋白和转铁蛋白受体基因表达均显著增加,表明运动训练造成的运动性低血色素大鼠肌肉中铁代谢紊乱,肌肉应激性使转铁蛋白以及转铁蛋白受体表达增加,以促进肌肉对铁的吸收;运动性低血色素大鼠肝脏组织中转铁蛋白基因表达均显著下降,这可能是由于肝脏铁储量增加而导致的转铁蛋白基因表达的适应性的降低有关;运动训练导致运动性低血色素大鼠转铁蛋白和转铁蛋白受体表达具有组织特异性,转铁蛋白受体基因表达与组织铁代谢关系密切.     

酸性环境抑制视网膜水平细胞NMDA受体活动

目的:研究酸性环境对NMDA受体的影响;方法:在分离的视网膜水平细胞上,施加不同pH值的含有NMDA和Glycine的Ringer液,测量NMDA受体电流;结果:低pH值对NMDA受体活动有抑制作用,半效抑制效应的pH值为6.6;结论:低pH值条件下,NMDA受体受到抑制,提示缺血等病理条件下的酸性环境抑制NMDA受体,起到保护作用。     

Expression of a fusion protein of human ciliary neurotrophic factor and soluble CNTF-Receptor and identification of its activity

INTRODUCTIONCiliaryneurotrophicfactor (CNTF)isanat urallyoccurringproteinwithamolecularmassofapproximately 2 2kDa .Itisexpressedinglialcellswithinthecentralandperipheralnervoussystems.CNTFstimulatesgeneexpression ,cellsurvivalordifferentiationinavarietyof…     

咳嗽机制的研究进展

本文总结了咳嗽受体和有关传入神经通路研究的进展。在咽喉部和气道中存在咳嗽受体，经有髓纤维或无髓纤维传到中枢整合后产生咳嗽。其发病机制可能与气道粘膜损害或／和粘膜下传入神经密度有关。     

SEB超抗原受体拮抗剂在大肠杆菌中的高效可溶性表达、纯化及活性初步研究

目的:在大肠杆菌(BL21)中构建可溶性表达的金黄色葡萄球菌B型肠毒素(SEB)受体拮抗剂。方法:首先确定SEB受体桔抗剂的基因序列,然后用含有SEB受体桔抗剂的基因序列重组质粒表达载体PGEX-4T-1转化大肠杆菌BL21(DE3),利用IPTG诱导表达获得蛋白,产物经GST柱纯化后,利用ELISA检测其与SEB的结合能力并进行其体内外药效学实验。结果:该质粒成功转化为可溶性表达,ELISA结果显示表达产物可与SEB特异性结合。结论:本研究成功对SEB受体拮抗剂GST可溶性表达并对其活性进行初步分析。     

The Remarkable Roles of the Receptor for Advanced Glycation End Products (RAGE) and Its Soluble Isoforms in COVID-19: The Importance of RAGE Pathway in the Lung Injuries

The respiratory symptoms of acute respiratory distress syndrome (ARDS) in the coronavirus disease 2019 (COVID-19) patients is associated with accumulation of pre-inflammatory molecules such as advanced glycation end-products (AGES), calprotectin, high mobility group box family-1 (HMGB1), cytokines, angiotensin converting enzyme 2 (ACE2), and other molecules in the alveolar space of lungs and plasma. The receptor for advanced glycation end products (RAGEs), which is mediated by the mitogen-activated protein kinase (MAPK), plays a critical role in the severity of chronic inflammatory diseases such as diabetes mellitus (DM) and ARDS. The RAGE gene is most expressed in the alveolar epithelial cells (AECs) of the pulmonary system. Several clinical trials are now being conducted to determine the possible association between the levels of soluble isoforms of RAGE (sRAGE and esRAGE) and the severity of the disease in patients with ARDS and acute lung injury (ALI). In the current article, we reviewed the most recent studies on the RAGE/ligands axis and sRAGE/esRAGE levels in acute respiratory illness, with a focus on COVID-19–associated ARDS (CARDS) patients. According to the research conducted so far, sRAGE/esRAGE measurements in patients with CARDS can be used as a powerful chemical indicator among other biomarkers for assessment of early pulmonary involvement. Furthermore, inhibiting RAGE/MAPK and Angiotensin II receptor type 1 (ATR1) in CARDS patients can be a powerful strategy for diminishing cytokine storm and severe respiratory symptoms.     

Effects of atorvastatin on progression of diabetic nephropathy and local RAGE and soluble RAGE expressions in rats

Objective  

Advanced glycation end-products (AGEs) exert inflammatory and oxidative stress insults to produce diabetic nephropathy mainly through the receptor for AGEs (RAGE). This study aimed to assess the effect of atorvastatin on diabetic nephropathy via soluble RAGE (sRAGE) and RAGE expressions in the rat kidney.     

消化道恶性肿瘤雌孕激素受体与癌胚抗原的相关性研究

利用酶联素和组化法和ABC免疫组化法进行88例消化道恶性肿瘤的雌激素受体(ER)、孕激素受体(PR)和癌胚抗原(CEA)的相关性研究。结果表明:(1)ER、PR水平与组织学分级呈负相关性。(2)低分化腺癌CEA阳性率最高,且淋巴结转移者CEA均为阳性,提示CEA与肿瘤的恶性度呈正相关。因此性激素受体和CEA提供了肿瘤组织不同的生物学信息,同时检测ER、PR及CEA水平对分泌治疗,手术范围及判断预后更有价值。     

Sequential Profiling of Anti-SARS CoV-2 IgG Antibody in Post COVID-19 Patients

Upon SARS CoV-2 infection, humoral immune system triggers production of anti-SARS CoV-2 IgM and IgG antibodies. Currently, antibodies against SARS CoV-2 spike protein receptor binding domain play a central role in disease protection, making them potential target for in vitro diagnostics applications. This study determines the expression level and sustainability of anti-receptor binding domain (RBD) SARS CoV-2 IgG in post COVID-19 patients. Anti-RBD SARS CoV-2 IgG antibodies in patient serum were analysed by standardised indirect ELISA using SARS CoV-2 spike receptor binding domain protein and HRP conjugated anti-human IgG antibody (anti-h IgG). The study was conducted using 35 adult patient samples with confirmed SARS CoV-2 infection. Additionally, correlation between antibody response after each stage and disease symptoms in post COVID-19 patients were studied. Maximum antibody titre was seen at Day 40 and decreased relatively to Day 180 in antibody positive samples when compared with controls. Overall, more IgG antibody expression is observed in patients who suffered from loss of smell and taste at Day 40. 71% of the positive subjects in this study showed high SARS CoV-2 IgG antibody concentration of above 10 ng/mL and 37% showed strong antibody concentration above 20 ng/mL at the peak of seroconversion.     

用分子动力学模拟Ryanodine受体中小肽的功能和结构

Ryanodine受体(骨骼肌RyR1)是目前为止克隆的最大的离子通道,其341位点突变会引起人恶性高热等疾病.应用分子动力学模拟给出RyR1的341位点附近肽段"GESLCFVQHV"的能量最小构象,说明能量最小化后其结构已经达到最稳定状态,即功能结构.