Serum lipids and malondialdehyde levels in primiparous patients with pregnancy induced hypertension

Background  Pregnancy induced hypertension (PIH) contributes to 15.6% maternal mortality in India. In Behrampur, Orissa, maternal deaths due to PIH was 32%, which is twice the national incidence. Hence in this population, some factors associated with severity of PIH were studied. Serum lipid concentrations and malondialdehyde (MDA) levels were correlated with severity of PIH and birth weight of the neonate. Patients & Methods  70 primiparous PIH patients and 20 healthy controls were studied. Serum lipids and MDA were estimated. Maternal blood pressures and birth weights of the neonate were recorded. Results and Conclusion  Serum cholesterol, triglyceride, LDL, VLDL and MDA were significantly elevated in primiparous PIH patients when compared to control subjects. Average birth weight of babies born to mothers with PIH was less than those born to control subjects. The most significant factor was that in this geographical area, 26% of the primiparous patients with PIH were below 20 years of age while only 15% of the controls were less than 20 years, indicating that younger maternal age was a contributing factor to PIH.     

Correlation between chemical components of billary calculi and bile & sera and bile of gallstone patients

Total cholesterol, total bilirubin, calcium, oxalate, inorganic phosphate, magnesium, iron, copper, sodium and potassium were analyzed quantitatively in gallstones, bile of gall bladder and sera of 200 patients of cholelithiasis (52 cholesterol, 76 mixed and 72 pigment stone patients) and their contents were correlated between calculi and bile and sera and bile in these three type of stone patients. A significant positive correlation was observed between total cholesterol, total bilirubin of calculi and bile, copper of bile and sera of cholesterol stone patients, copper of calculi and bile, total bilirubin, oxalate, magnesium, potassium of sera and bile of pigment stone patients and oxalate and iron of stone and bile, total bilirubin, oxalate, sodium of sera and bile of mixed stone patients. A significant negative correlation was found between magnesium of serum and bile of cholesterol stone patients, oxalate of calculi and bile of pigment stone patients and magnesium of serum and bile of mixed stone patients.     

Determination of immunoreactivity of<Emphasis Type="Italic">Plasmodium falciparum</Emphasis> antigens,serum dilutions and biomaterials

Immunoreactivity properties of serum dilutions andPlasmodium falciparum malaria antigens were measured and compared by ELISA technique using different ELISA plates to evaluate the role of antigens and serum dilutions for optimum binding. Also effort has been made to see the effect of reaction surface and material i.e. ELISA plates for binding capacity. Serological properties were estimated by ELISA methods for detection of malaria and determination of immunological characteristics. Three Pf antigens (PfAg) i.e. ring infected erythrocyte surface antigen: AR-1 (RESA), histidine-rich protein 2 antigen (HRP-2) and glycophospholipid antigen (grown and developed Pf antigen from PSJ-M strain): GPL1 have been used for serological testing of human blood samples by Enzyme Link Immunosorbant Assay (ELISA). 1∶100, 1∶1000 and 1∶10000 dilutions of Pf positive and negative serum (50 samples in each group) and 1∶1000 dilution of Pf antigens were used to measure immunoreactive properties by ELISA method. Result of PfAg-serum immunoreactivity study showed that GPL1 has the highest degree of immuno binding reactivity compared to other Pf antigens. HRP-2 and RESA antigens showed no significant difference to each other. Study also found that Costar and Fastec ELISA plates have a better Ag−Ab binding capability compared to immulon and Falcon plates at all dilutions of serum. Serum dilution of 1∶100 showed best binding and reactivity with Pf antigens followed by 1∶1000 and 1∶10000 showed lowest reactivity.     

Study on the serum levels of soluble intercellular adhesion molecule-1 (sICAM-1) in patients with Helicobacter pylori Infection

ＩＮＴＲＯＤＵＣＴＩＯＮＳｉｎｃｅｔｈｅｆｉｒｓｔｓｕｃｃｅｓｓｆｕｌｉｓｏｌａｔｉｏｎｏｆＨ .ｐｙ ｌｏｒｉｉｎ 1 982 ,Ｈ .ｐｙｌｏｒｉｉｎｆｅｃｔｉｏｎｈａｓｂｅｅｎｆｏｕｎｄｔｏｂｅａｓｓｏｃｉａｔｅｄｗｉｔｈｖａｒｉｏｕｓｄｉｓｅａｓｅｓｉｎｃｌｕｄｉｎｇｃｈｒｏｎｉｃｇａｓｔｒｉｔｉｓ,ｐｅｐｔｉｃｕｌｃｅｒｄｉｓｅａｓｅｓ(Ｄｏｏｌｅｙｅｔａｌ.,1 989;Ｇｒａｈａｍｅｔａｌ.,1 992 ) ,ａｎｄｇａｓｔｒｉｃｎｅｏｐｌａｓｍｓ(Ｂａｙｅｒｄｏｒｆｆｅｒｅｔａｌ.,1 995) .Ｉｔｗａｓｓｕｇｇｅｓｔ…     

Role of bilirubin, vitamin C and ceruloplasmin as antioxidants in coronary artery disease [CAD]

A study has been carried out on 250 CAD patients to see how the serum levels of three antioxidants i.e., Vitamin C, Bilirubin and Ceruloplasmin are related to the CAD risk factors and characteristics in these patients. The number of severe category CAD patients declined by 7–18% with increasing serum levels of the three antioxidants and, triple vessel disease declined by 14–20%. A decline of 39% in Myocardial Infarction (MI) occurred with increasing serum Ceruloplasmin. Serum Ceruloplasmin was significantly lower in the MI group compared to the non MI group CAD patients. There was a steady and a significant decline in the mean values of serum levels of cholesterol, Triglyceride, VLDL cholesterol along with BMI with increasing serum level. The study indicates that with increasing serum levels of the three antioxidants studied, the % MI along with % Triple vessel disease and severity of CAD goes down suggesting that the modifiable risk factors have to be suitably modified in order to maintain a reasonably high level of these antioxidants, as the risk factors are inversely related to the serum antioxidant levels.     

糖尿病患者血清中微量元素的分析研究

对 ６５例糖尿病患者进行了血清中微量元素锌、铜、铁、铬、锰的含量测定,并与正常对照组进行了比较．结果表明,病例组的糖尿病患者血清锌、铁、铬、锰均低于对照组,其中锌、铬、锰水平与对照组比较差异显著（Ｐ＜０．０５）;血清铁虽低于对照组,但无统计学意义．血清钢水平则高于对照组,铜水平与对照组比较差异显著（Ｐ＜０．０５）．     

Serum ionised calcium in birth asphyxia

Serum total and ionised calcium levels were measured at birth and at 48 hours in 25 term neonates with birth asphyxia (one minute APGAR score of 6 or less) and in 25 normal term neonates (one minute APGAR score of 7 or more). Infants were categorised into two groups TAGA (term appropriate for gestational age) and TSGA (term small for gestational age). Asphyxiated infants had significantly lower serum total and ionised calcium values at birth as well as at 48 hours. Abnormal clinical features were observed in 48% of asphyxiated infants. Low ionised calcium was detected in symptomatic babies, who had otherwise normal total calcium values. Due to hyocalcemia especially ionised calcium in asphyxiated infants and high frequency of functional derangement associated with this hypocalcemioa, serial monitoring of serum isonised calcium levels is necessary.     

Predictive value of serum thyroglobulin in treated patients with differentiated thyroid carcinoma

An in-house radioimmuno assay for serum thyroglobulin was developed in our laboratory and compared its relative sensitivity with that of whole body scan in the detection of residual tumour or metastases and evaluated the predictive value of serum thyroglobulin in the clinical course of the disease. Ninety six patients after thyroidectomy were followed up for a maximum period of five years in this study. The sensitivity and specificity of serum thyroglobulin were found to be close to that of whole body scan (85% and 94% respectively). According to this study, a serum thyroglobulin >40 ng/ml can differentiate between patients with metastases and those in remission. Serum thyroglobulin can replace whole body scan during the subsequent follow-up if the patient had concordant whole body scan and serum thyroglobulin during initial assessment.     

Development of a highly sensitive direct microtiter plate ELISA for human serum testosterone

A simple, highly sensitive, direct, competitive ELISA for human serum testosterone has been indigenously developed. Specific antisera against testosterone were raised in rabbits using testosterone—3carboxymethyl oxime (CMO)—bovine serum albumin (BSA) as the antigen. For the enzyme conjugate, testoterone—3CMO was coupled with horse raddish peroxidase by the active ester method. The standard curve covered a wide range from 3.9 pg/ml to 500 pg/ml. The inter and intra-assay variation were found to be low and within the acceptable limits. Specificity and accuracy for the assay was established by having negligible crossreactivity with the related steroids and an excellent parallelism between the sample and standard dilution curve. Samples measured by RIA and ELISA showed very high degree of correlation (r=0.991).