Study of C-Reactive Protein and Myocardial Infarction in the Indian Population

To analyse the association of high sensitivity C-reactive (hsCRP) protein levels and −717A/G single nucleotide polymorphism of CRP with acute myocardial infarction (AMI) in the Indian population. Study population included 100 MI cases wherein 32 patients had experienced previous MI (MI-Group-1), 68 MI cases were recruited at presentation (MI-Group-2) and equal number of age and gender matched healthy individuals. hsCRP levels were determined by ELISA and genotyping of −717A/G was carried out by polymerase chain reaction-based restriction digestion method. The −717A/G genotypes did not influence hsCRP level and their distribution did not differ between groups. However, in the present study hsCRP demonstrated significant correlation with BMI in controls of both the genders and with triglycerides in females of AMI at presentation who otherwise are with low risk profile. Identifying traditional risk factors associated with inflammation may help in controlling the acute event.     

有氧能力关联基因多态与技巧类滑雪运动员跨项选材

目的：研究优秀技巧类滑雪运动员中4个有氧能力关联多态基因ACE、GLUT4、ADRB2和PPARGC1A的分布频率，及基于4个优势等位基因的基因型总分（TGS），探索上述多态基因与TGS作为我国优秀技巧类滑雪运动员跨项选材分子标记的可行性。方法：应用基质辅助激光解吸附电离飞行时间质谱检测技术，对68名中国北方汉族优秀技巧类滑雪运动员与138名中国北方汉族普通大学生ACE基因rs4340位点、GLUT4基因rs5418位点、ADRB2基因rs1042713位点和PPARGC1A基因rs8192678位点进行解析。计算并比较运动员与对照组在上述4个位点的TGS。结果：在上述4个多态位点，运动员与对照组间的基因型和等位基因频率无显著性差异（P>0.05）；两组人在携带的优势等位基因数量、TGS分值范围、平均TGS分值及TGS分值的分布频率上也均无显著性差异（P>0.05）。结论：ACE基因rs4340位点、GLUT4基因rs5418位点、ADRB2基因rs1042713位点、PPARGC1A基因rs8192678位点多态性和基于上述4个位点的TGS均无法作为中国北方汉族技巧类滑雪运动员的有氧能力跨项分子选材标记。     

面向对象程序设计浅析

通过分析面向对象程序设计的基本思想和技术特点及他们之间的区别和联系,介绍了面向对象程序设计的优点和发展趋势。     

构造器内部多态方法的行为研究

概述Java中的多态、多态方法，并通过实例阐述Java中多态的实现机制及本质。进而对构造器中调用多态方法产生的错误结果进行了分析并得出结论。     

Genetic polymorphism analyses of a novel panel of 19 X-STR loci in the Chinese Uygur ethnic minority

The population genetic data and forensic parameters of 19 X-chromosome short tandem repeat (X-STR) loci in Chinese Uygur ethnic minority are presented. These loci were detected in a sample of 233 (94 males and 139 females) unrelated healthy individuals. We observed 238 alleles at the 19 X-STR loci, with the corresponding gene frequencies spanning the range from 0.0021 to 0.5644. After Bonferroni correction (P>0.0026), there were no significant deviations from Hardy-Weinberg equilibrium. The cumulative power of discrimination in females and males, and the probability of exclusion of the 19 X-STR loci were 0.999 999 999 999 999 999 998 091, 0.999 999 999 999 966, and 0.999 999 986 35, respectively. The cumulative mean exclusion chance was 0.999 999 992 849 in deficiency cases, 0.999 999 999 999 628 in normal trios, and 0.999 999 998 722 in duo cases. The high value of the forensic parameters mentioned above revealed that the novel panel of 19 loci had important values for forensic applications in the Uygur group.     

A taste of functional programming — 2

Functional programming has its roots in Alonzo Church’s lambda calculus. In the first part¹ of this article, we explored some basic notions of functional programming using the language Haskell. We now examine some more advanced concepts, including polymorphism, infinite data types and computations, and user-defined data types. Madhavan Mukund has been on the Computer Science faculty at Chennai Mathematical Institute since 1992, where he is currently Professor. His research interests are primarily in formal methods for specifying and verifying computing systems. He is the Secretary of the Indian Association for Research in Computing Science (IARCS) and the National Coordinator of the Indian Computing Olympiad.     

对面向对象程序设计的探讨

认真区分面向对象程序设计与面向过程程序设计,了解面向对象程序设计的特征,列出了面向对象程序设计的优点.     

MSTN基因rs7570532多态性对全身振动训练干预绝经后女性体成分效果的影响及分子调控机制

摘要：目的：对绝经后女性进行为期3个月的全身振动训练,观察其对绝经后女性体成分的影响,分析肌肉生长抑素（myostain, MSTN）基因rs7570532位点多态性对振动训练干预体成分效果的影响,并探讨其分子调控机制。方法：从社区招募60名汉族健康绝经后女性（58.87±4.70岁）,对其进行为期3个月的全身振动训练,分别在振动训练干预前、后测试受试者的身高、体重、体重指数（body mass index, BMI）以及体成分,体成分的测试采用Prodigy Lunar双能X线骨密度仪,测试指标包括全身脂肪百分比（Percentage of fat mass, FM%）、全身脂肪含量（fat mass, FM）和全身瘦体重含量（lean mass, LM）。采用飞行质谱法对MSTN基因rs7570532多态位点进行基因分型;采用荧光素酶报告系统检测不同基因型重组质粒报告基因的相对活性。结果：绝经后女性经过3个月的全身振动训练,其体重、BMI、FM%和FM均显著下降,LM无显著变化。受试者基因分型结果显示,34人为TT型,19人为TC型,仅有2人为CC型,受试者基因型频率符合H-W遗传平衡定律。经过3个月的全身振动训练,TT型受试者的FM%显著下降（36.4±5.4, 35.2±5.0, P<0.01）,FM显著下降（22.6±5.5, 21.7±5.3, P<0.01）,LM显著上升（36.8±3.6, 37.2±3.9, P<0.05）;TC+CC型受试者的体重和体成分各指标均无显著变化。在控制干预前受试者各项指标的基础值后,TT型和TC+CC型受试者之间FM%和LM具有显著差异,FM的差异性消失。pGL3-promoter-MSTN-T重组质粒报告基因的相对活性（3.30±1.121）显著高于pGL3-promoter-MSTN-C（1.76±0.92, p<0.01）,且2者均显著高于空质粒对照组（0.81±0.17, p<0.01）。结论：1)3个月的全身振动训练可显著改善绝经后女性的体重和体成分;2)MSTN基因rs7570532位点多态性显著影响振动训练干预体成分的效果;3)且此位点多态性可显著影响下游基因的表达活性,其可能是振动训练改善体成分效果个体差异的原因之一。     

Methylenetetrahydrofolate reductase C677T polymorphism predicts response and time to progression to gemcitabine-based chemotherapy for advanced non-small cell lung cancer in a Chinese Han population

Objective:The aim of this study was to evaluate the association between the methylenetetrahydrofolate reductase(MTHFR) C677T excision repair cross-complementation group 1(ERCC1) genetic polymorphisms and the clinical efficacy of gemcitabine-based chemotherapy in advanced non-small cell lung cancer(NSCLC).Methods:A total of 135 chemonaive patients with unresectable advanced NSCLC were treated with gemcitabine/platinum regimens.The polymorphisms of MTHFR C677T,ERCC1 C8092A,and ERCC1 C118T were genotyped using the TaqMan methods.Results:The overall response rate was 28.9%.Patients with MTHFR CC genotype had a higher rate of objective response than patients with variant genotype(TT or CT)(41.2% versus 19.1%,P=0.01).Median time to progression(TTP) of patients with MTHFR CC genotype was longer than that of patients with variant genotype(7.6 months versus 5.0 months,P=0.003).No significant associations were obtained between ERCC1 C118T and C8092A polymorphisms and both response and survival.Conclusions:Our data suggest the value of MTHFR C677T polymorphism as a possible predictive marker of response and TTP in advanced NSCLC patients treated with gemcitabine/platinum.     

β3肾上腺素受体的基因多态性与肥胖的关系及运动的影响

β3肾上腺素能受体在脂肪代谢中起着非常重要的作用。β3-AIR基因第64位密码子变异导致色氨酸被精氨酸取代,该变异与肥胖、胰岛素抵抗及2型糖尿病发病提早密切相关。相关研究表明,β3-AR基因Trp64Arg变异可能会干扰肥胖者的运动疗法。提示为肥胖患者制定运动处方时应考虑该变异的影响。