Hypertonic saline resuscitation reduces apoptosis of intestinal mucosa in a rat model of hemorrhagic shock

Objective: To investigate the early effects of hypertonic and isotonic saline solutions on apoptosis of intestinal mueosa in rats with hemorrhagic shock. Methods: A model of rat with severe hemorrhagic shock was established in 21 Spragne-Dawley (SD) rats. The rats were randomly divided into the sham group, normal saline resuscitation (NS) group, and hypertonic saline resuscitation (HTS) group, with 7 in each group. We detected and compared the apoptosis in small intestinal mucosa of rats after hemorrhagic shock and resuscitation by terminal deoxynucleotidyl transferase dUTP nick end labelling (TUNEL), FITC (fluo-rescein-iso-tbiocyanate)-Annexin V/PI (propidium iodide) double staining method, and flow cytometry. Results: In the early stage of hemorrhagic shock and resuscitation, marked apoptosis of small intestinal mucosa in the rats of both NS and HTS groups was observed. The numbers of apoptotic cells in these two groups were significantly greater than that in the sham group (P＜0.01). In the HTS group, the apoptic cells significantly decreased, compared with the NS group (P＜0.01). Conclusion: In this rat model of severe hemorrhagic shock, the HTS resuscitation of small volume is more effective than the NS resuscitation in reducing apoptosis of intestinal mucosa in rats, which may improve the prognosis of trauma.     

过氧化氢法处理镀锡含氰废水

对试用过氧化氢法对含氰电镀废水酸化回收后进行二次处理的试验情况、处理原理，生产影响因素及安全等进行了探讨，近半年的生产应用情况表明，该法具有工艺操作简单，投资省，成本低等优点，能容易地将含氰（CN^-）500mg/L的酸化回收尾液处理到0．5mg/L以下。     

Detection of Hydrogen Peroxide Photogenerating from a Hypocrellin B Derivative

Hypocrellins,includinghypocrellinA (HA)andhypocrellinB (HB) (Fig .1) ,arenewtypesofphoto sensitivepigments growingabundantlyinChina[1,2 ] .Theyexhibitseveraladvantagesoverhaematoporphyrinderivatives (HPDs) presentlyusedinclinics[3] .Howev er,hypocrellinsarelipophilicagentsandexhibitweakab sorptioninthe phototherapeuticwindow (6 0 0— 90 0nm) ,whichpreventsfurtherinvestigationsontheirbio logicalconsequencesandlimitstheirPDTapplicationclinically .Fig .1　Chemicalstructuresofhypocrellinpare…     

过氧化氢氧化苯甲醛合成苯甲酸的研究

在无溶剂、无相转移催化荆条件下,以过渡金属离子作为催化剂,碳酸钠作为碱性添加物,30％过氧化氢作为氧源,在常温搅拌下来催化氧化苯甲醛制备苯甲酸。最佳实验条件为：n（苯甲醛）／n（碳酸钠）／n（催化剂）,n（过氧化氢）为100：3：0．5：196,反应时间为1h,苯甲酸产率达84．2％。     

谷氨酸钠溶液514nm激光拉曼光谱受环境光影响的研究

探讨了环境光的存在对谷氨酸钠溶液的514nm绿色激光激发的拉曼光谱的影响。研究表明不同的环境光、自然光和室内荧光灯光均会对514nm激光拉曼光谱产生干扰,存在着特征干扰峰。自然阳光干扰较小;而荧光灯光表现为1127、2107cm^-1和2170cm^-1等3个明显尖锐的脉冲峰,不能忽略。建议在进行谷氨酸钠溶液514nm绿色激光拉曼光谱检测时,须在暗室或暗罩中进行,以完全隔离环境光的影响。     

气相色谱法测定面粉中过氧化苯甲酰

面粉中的过氧化苯甲酰经乙醚提取浓缩后,被碘化钾还原反应生成苯甲酸,利用气相色谱法对其进行分离测定,并与标准品比较定性、定量.该方法的最低检出含量为0.3 mg/kg,加标回收率在90%～99%之间.该方法分离效率高,样品用量少,检测灵敏度高.     

Expression and proteolytic activity of calpain in lens epithelial cells of oxidative cataract

Objective: To study the role of calpain in the mechanism of oxidative cataract through detecting the level of intracellular free Ca2 , the expression and proteolytic activity of calpain in the lens epithelial cells (LECs) of H2O2-induced cataract. Methods: Rat lenses were cultured in vitro and cataract was induced by H2O2. The level of intracellular free Ca2 was measured by fluorescence determination with fura-2/AM. The expression of m-calpain protein in LECs was detected with immunohistochemical method. The proteolytic activity in LECs was measured using a fluorogenic synthetic substrate. Results: There were significant differences of the level of intracellular free Ca2 (P=0.001, 0.000, 0.000), the expression of m-calpain (P=0.001, 0.000, 0.000) and the proteolytic activity of calpain (P=0.001, 0.000, 0.000) between H2O2-induced and control group at 6, 12 and 24 h, respectively. Conclusions: H2O2 can increase intracellular free Ca2 , then enhance the expression and proteolytic activity of calpain which may play a role in the mechanism of oxidative cataract of rat.     

对甲苯胺合成的均匀设计方法

本文用均匀设计方法考察了甲苯胺的优化工艺条件.由对硝基甲笨和结晶硫化钠直接反应制备对甲苯胺,收率为88.9%.     

琼脂糖固定化过氧化氢酶的电化学性能研究

利用琼脂糖（agarose）将过氧化氢酶（Cat）固定在热裂解石墨电极表面,制备Cat—agarose膜修饰电极．包埋在琼脂糖中妁过氧化氢酶与电极直接传递电子,在pH6．0的缓冲溶液中可得一对可逆的氧化还原峰,式电势为-0．343V（vs SCE）,这是过氧化氢酶辅基血红素Fe（Ⅲ）／Fe（Ⅱ）电对的氧化还原．其式电势随溶液的pH值增加而负移且成线性关系,直线斜率为-36．8mV／pH,说明过氧化氢酶的电子传递过程伴随有质子的转移．过氧化氢酶修饰电极．在16．5—278μmol／L的范围内,催化电流与过氧化氢浓度成线性关系．     

钒酸盐对蟾蜍心室肌FRP和兴奋性的影响

本实验采用累积给药法，以偏钒酸钠（ＮａＶｏ３）灌流蟾蜍心室肌条，观察ＮａＶｏ３对心室肌功能性不应期（ＦＲＰ）和兴奋性的影响。结果：１０—１６０μｍｏｌ·Ｌ－１浓度的ＮａＶｏ３不同程度地延长心室肌ＦＲＰ，当灌流液ＮａＶｏ３浓度为８０μｍｏｌ·Ｌ－１时，心室肌ＦＲＰ的廷长与对黑浓度呈现显茗性差异（Ｐ＜０．０５），ＮａＶｏ３浓度为１６０μｍｏｌ·Ｌ－１时，其差异更显著（Ｐ＜０．０１）。ＮａＶｏ３浓度为４０μｍｏｌ·Ｌ－１和８０μｍｏｌ·Ｌ－１时，显著提高心室肌的兴奋性。