Culture of Spirulina platensis in human urine for biomass production and O2 evolution

Attempts were made to culture Spirulina platensis in human urine directly to achieve biomass production and O2 evolution, for potential application to nutrient regeneration and air revitalization in life support system. The culture results showed that Spirulinaplatensis grows successfully in diluted human urine, and yields maximal biomass at urine dilution ratios of 140-240. Accumulation of lipid and decreasing of protein occurred due to N deficiency. O2 release rate of Spirulina platensis in diluted human urine was higher than that in Zarrouk medium.     

极大螺旋藻藻蓝蛋白基因在巴斯德毕赤酵母X-33中表达的研究

通过PCR技术克隆了极大螺旋藻藻蓝蛋白基因，构建了克隆载体和表达载体，并将该基因导入巴斯德毕赤酵母X-33基因组，筛选了能够有效表达藻蓝蛋白基因的毕赤酵母工程菌株PC8，从而为发酵法生产藻蓝蛋白奠定了基础。     

用螺旋藻吸收去除尿液中氮、磷和有机物

针对资源化回收利用尿液中主要营养物质的问题,在实现尿液培养螺旋藻的基础上,探讨螺旋藻对尿液中主要营养物质氮、磷和有机物的吸收和去除.螺旋藻对尿液中氮素的吸收与螺旋藻生物量的变化保持一致,最大吸收率可达59.92%;尿液中约64.15%的磷被去除,其中螺旋藻的理论吸收约占39.24%,其余24.91%可能以沉淀形式离开尿液;螺旋藻对尿液中有机物的吸收最高可达58.4%,尿液中的有机物有助于螺旋藻的生长.研究结果为尿液的资源化回用开辟1种途径.     

螺旋藻基因工程研究进展

近年来随着螺旋藻研究的深入及分子生物学的渗透，有关螺旋藻基因工程的研究迅速发展并取得了较满意的成果。本文全面综述了这些研究成虹并提出了今后螺旋藻基因工程研究发展的方向；通过重组DNA技术构建螺旋藻新品种和实现螺旋藻天然产物基因工程生产。     

极大螺旋藻(Spirulina maxima)藻蓝蛋白基因的克隆及其同源性分析

藻蓝蛋白是红藻和蓝绿藻中一种重要的捕光色素蛋白．克隆了编码极大螺旋藻藻蓝蛋白α和β两个亚基的基因，序列分析表明该基因全长为1119bp．β亚基基因位于α亚基基因上游，两个亚基基因序列长度分别为519bp和489bp，中间被111bp的基因片段间隔．除编码α和β两个亚基的开放阅读密码框外，还存在两个潜在的开放阅读密码框，但这两个密码框的意义还不清楚．比较了该藻蓝蛋白氨基酸序列和来自于其他藻类的藻蓝蛋白氨基酸序列的同源性以及藻蓝蛋白α和β两个亚基之间氨基酸序列的同源性，总的来说其同源性在45．9％．99％之间，α和β两个亚基氨基酸序列同源性为27．1％．藻蓝蛋白包含许多疏水性氨基酸，这些疏水性氨基酸在藻胆蛋白的聚集过程中起着极为重要的作用．分析表明编码藻蓝蛋白α和β两个亚基基因的密码子显示出非对称性．     

螺旋藻的生物特性及其应用价值

概述了螺旋藻的生物学特性，并介绍了其营养价值和药用价值，以及在能源和环保方面的潜在应用价值，从而阐明了螺旋藻作为一种新的资源具有很高的开发利用价值。     

螺旋藻中脂肪酸的GC-MS分析

采用索式提取法对螺旋藻中的脂肪油进行提取,并对脂肪酸进行甲酯化处理,利用气相色谱-质谱联用仪进行分析测定.螺旋藻共检测出6种脂肪酸,不饱和脂肪酸为4种,相对百分含量占检出所有脂肪酸的57.44%,其中,二十碳五烯酸（EPA）为12.17%,亚油酸为7.25%,5,8,11,14-二十碳四烯酸（花生四烯酸）为22.12%.     

Spirulina platensis aqueous extracts ameliorate colonic mucosal damage and modulate gut microbiota disorder in mice with ulcerative colitis by inhibiting inflammation and oxidative stress

Ulcerative colitis (UC) is a chronic and recurrent inflammatory bowel disease (IBD) that has become a major gastroenterologic problem during recent decades. Numerous complicating factors are involved in UC development such as oxidative stress, inflammation, and microbiota disorder. These factors exacerbate damage to the intestinal mucosal barrier. Spirulina platensis is a commercial alga with various biological activity that is widely used as a functional ingredient in food and beverage products. However, there have been few studies on the treatment of UC using S. platensis aqueous extracts (SP), and the underlying mechanism of action of SP against UC has not yet been elucidated. Herein, we aimed to investigate the modulatory effect of SP on microbiota disorders in UC mice and clarify the underlying mechanisms by which SP alleviates damage to the intestinal mucosal barrier. Dextran sulfate sodium (DSS) was used to establish a normal human colonic epithelial cell (NCM460) injury model and UC animal model. The mitochondrial membrane potential assay 3-‍‍(4,5-dimethylthiazol-2-yl)-2,‍5-diphenyltetrazolium bromide (MTT) and staining with Annexin V-fluorescein isothiocyanate (FITC)/propidium iodide (PI) and Hoechst 33258 were carried out to determine the effects of SP on the NCM460 cell injury model. Moreover, hematoxylin and eosin (H&E) staining, transmission electron microscopy (TEM), enzyme-linked immunosorbent assay (ELISA), quantitative real-time polymerase chain reaction (qPCR), western blot, and 16S ribosomal DNA (rDNA) sequencing were used to explore the effects and underlying mechanisms of action of SP on UC in C57BL/6 mice. In vitro studies showed that SP alleviated DSS-induced NCM460 cell injury. SP also significantly reduced the excessive generation of intracellular reactive oxygen species (ROS) and prevented mitochondrial membrane potential reduction after DSS challenge. In vivo studies indicated that SP administration could alleviate the severity of DSS-induced colonic mucosal damage compared with the control group. Inhibition of inflammation and oxidative stress was associated with increases in the activity of antioxidant enzymes and the expression of tight junction proteins (TJs) post-SP treatment. SP improved gut microbiota disorder mainly by increasing antioxidant enzyme activity and the expression of TJs in the colon. Our findings demonstrate that the protective effect of SP against UC is based on its inhibition of pro-inflammatory cytokine overproduction, inhibition of DSS-induced ROS production, and enhanced expression of antioxidant enzymes and TJs in the colonic mucosal barrier.     

钝顶螺旋藻藻胆体核心与藻蓝蛋白的重组

用钝项螺旋藻进行了藻胆体核心和藻蓝蛋白重组的研究.在室温荧光发射光谱中,藻胆体核心-藻蓝蛋白重组复合物的荧光发射峰位于663nm,与藻蓝蛋白的室温荧光发射峰643nm和藻蓝蛋白 藻胆体核心混合物(未重组)的室温荧光发射峰648nm相比,荧光发射峰向长波方向发生了移动,并向藻胆体核心室温荧光发射峰664nm逼近.说明藻蓝蛋白与藻胆体核心发生了重组,而且重组效果较好.在液氮温度荧光发射光谱中,其结果也表明藻蓝蛋白与藻胆体核心发生了重组.     

藻菌混合固定化及其对亚甲基兰的脱色研究

将盐泽螺旋藻和细菌混合固定化,比较对亚甲基兰的脱色情况．结果表明：单独固定化藻对亚甲基兰作用4d,脱色率达到80．9％,而在混合固定化条件下,24h即超过了单一固定化藻的脱色效果,脱色率达到83％,作用4d后脱色率达到92．9%．可以认为细菌的加入提高了盐泽螺旋藻对亚甲基兰的抗毒性,并在较大程度上提高了固定化盐泽螺旋藻对亚甲基兰的脱色能力．