Improved elastase production by<Emphasis Type="Italic">Bacillus</Emphasis> sp. EL31410 —Further optimization and kinetics studies of culture medium for batch fermentation

An efficient culture medium producing a bacterial elastase with high yields was developed further following preliminary studies by means of response surface method. Central composite design (CCD) and response surface methodology were applied to optimize the medium constituents. A central composite design was used to explain the combined effect of three medium constituents, viz, glucose, K₂HPO₄, MgSO₄·7H₂O. The strain produced more elastase in the completely optimized medium, as compared with the partially optimized medium. The fitted model of the second model, as per RSM, showed that glucose was 7.4 g/100 ml, casein 1.13 g/100 ml, corn steep flour 0.616 g/100 ml, K₂HPO₄ 0.206 g/100 ml and MgSO₄·7H₂O 0.034 g/100 ml. The fermentation kinetics of these two culture media in the flask experiments were analyzed. It was found that the highest elastase productivity occurred at 54 hours. Higher glucose concentration had inhibitory effect on elastase production. At the same time, we observed that the glucose consumption rate was slow in the completely optimized medium, which can explain the lag period of the highest elastase production. Some metal ions and surfactant additives also affected elastase production and cell growth. Project (No. 300024) supported by the Natural Science Foundation of Zhejiang Province, China     

试论大学体育教学中的心理健康教育

通过对大学体育教学中学生心理障碍的分析，强调了开展心理健康教育的重要性，并提出了相应对策，以提高体育教学效果。     

Improved production of spiramycin by mutant Streptomyces ambofaciens

INTRODUCTION which the hydroxyl group is acetylated, and spi- ramycin III in which the same position is propio- Spiramycin belongs to the macrolide antibi- nylated (Omura et al., 1979). The addition of short-otics group. The molecular structure of spiramycin chain fatty acids stimulates the production of spi-consists of a 16-membered branched lactonic ring …     

THE GENERATING THEORY OF THE FLUTES''''PROFILE OF SPECIAL REVOLVING TOOLS WITH CONSTANT-SPIRAL-ANGLED EDGES

The manafacturing theory proposed in this paper is composed of the se-lection of the machining type(motions and adjustments)and the determination of thefunctions of motion parameters.The paper shows that the special revolving tools withconstant spiral angled egdes can be ground in the[x y (?)]or[x y z (?)]systembased on the conjugate theory and differential geometry.The functions of motion pa-rameters in these two systems are deduced.     

纤维素降解菌ZN-206的分离鉴定

采用连续稀释分离法和平板划线分离法的方法,利用以羧甲基纤维素钠为碳源的筛选培养基,从堆肥土壤中共分离获得具有纤维素酶降解能力的菌株15株,其中,菌株ZN-206对纤维素降解能力最强.根据ZN-206菌株的形态特征、培养性状,生理生化指标、细胞壁组成成分及16SrDNA序列进行系统的研究,将菌株鉴定为禾粟链霉菌(Streptomyces graminearus).     

油茶组织培养研究进展

本文对目前我国油茶的组织培养研究现状进行总结,从培养条件、培养基、外植体类型、外源激素等方面分析油茶组织培养的特点和影响因素,提出油茶组织培养的研究方向及组织培养技术应用中注意的问题。     

高校体育股份制俱乐部系统管理模式的构建

从系统科学的角度出发，运用管理学方法，构建了高校体育股份制俱乐部的系统管理模式，从而有效解决高校体育教学和高校体育的社会化问题，形成科学的“学校—社区—社会”一体化的全面健身服务体系。     

慢性乙型肝炎病毒感染学生体育锻炼的现状

运用问卷调查法对高校慢性乙肝感染学生的体育锻炼意识、目的、行为和技能进行了调查分析,结果表明,慢性乙肝病毒感染学生担心体育锻炼损伤身体,体育锻炼意识及达标率明显低于正常学生,体育锻炼目的主要是应付考试。有必要对这些学生加强卫生宣传教育,改革教学方法。     

在土壤杆菌ZX09中透明颤菌血红蛋白的表达提高索拉胶的产量

研究目的：提高胞外多糖索拉胶的产量。 创新要点：透明颤菌血红蛋白基因在质粒上表达,不加抗生素发酵生产索拉胶。 研究方法：将透明颤菌血红蛋白基因构建到广谱质粒pCM158上得到重组质粒pCM158-vgb,重组质粒转入土壤杆菌ZX09中,使透明颤菌血红蛋白在土壤杆菌ZX09中表达。 重要结论：在土壤杆菌ZX09中颤菌血红蛋白的表达解决了发酵过程中氧受限的问题,提高了宿主菌的呼吸速率、蔗糖酶活性和黏度,将发酵产物索拉胶的产量提高了30％。     

芽孢杆菌葡萄糖脱氢酶结构与功能研究进展

芽孢杆菌葡萄糖脱氢酶在辅酶再生方面具有良好的应用前景,因而受到了广泛关注.本文结合葡萄糖脱氢酶的三维结构和生理生化特性,尝试建立起其结构与功能方面的相关关系,为葡萄脱氢酶的体外改造提供有价值的信息,并指出了目前应用上存在的问题,对发展趋势做了展望.