温度、pH及碳氮源对产色素青霉菌株（Penicilliumsp．）生长的影响

PSO312是从土壤分离的1个青霉菌株,生长中能产生不同酸碱性条件下显黄色与紫红色等天然色素物质．通过菌株固体平板上恒温培养,168h测定菌落直径的方法研究了温度、pH及碳、氮源对菌株（Penicilliumsp．）生长的影响．研究表明31℃为菌株生长的最适温度．31℃培养以pH2—3的最快,pH8—9生长次之,24℃培养以pH8—9生长最快,pH3—4其次．菌株以蛋白胨4g／L、牛肉膏4g／L、酵母膏2g／L及尿素2g／L为氮源培养的菌落直径分别为55．75mm、51．5mm、55．5mm及25．3mm．在（NH4）2SO4 2g／L、NH4NO3 3g／L、NaNO3 4g／L、KN034g／L用量之上培养生长最快,菌落直径分别为35mm、34mm、35mm、及32mm．葡萄糖、蔗糖用量30g／L、果糖20g／L及乳糖25g／L的生长速率最快,菌落直径分别为45mm、38mm、38mm及39mm．丙三醇与淀粉25g／L培养的菌落直径均为39mm．     

Improvement of Desferrioxamine B Production of Streptomyces pilosus ATCC 19797 With Use of Protease Inhibitor and Minerals Related to Its Activity

The relationship between protease and Desferal production was assayed. Experiments were performed using a cultivation of Streptomyces pilosus ATCC 19797 in soybean broth medium containing 2% soybean flour and 2% mannitol. The metabolism of the trihydroxamic acid sidrophore desferrioxamine B and protease production by a S. pilosus in nine continues days after culture were investigated as well as the effect of protease inhibitors was examined. It is found that the Desferal formation decreased with increased protease production. Also the effect of protease inhibitors and minerals in determined day of protease production in the culture medium by S. pilosus has been investigated.     

世界一流大学的普世精神及其悖论

世界一流大学存在某些普遍和共同的特征与规训，在风起云涌的全球化浪潮中，这些特征和规训往往成为评判世界一流大学的重要尺牍而被竞相标榜，大学在迎合这种普世化的同时，也成为一种推行普世理念的文化工具。但是，这些普世理念在理论上存在诸多悖论，在实践中陷入两难，发展中国家的世界一流大学建设，在向西方世界一流大学趋同和靠近的过程中，必须保持个性和特色，尤其要注重“本土生长”的内生因素。     

转基因鱼腥藻7120流加培养的研究

流加培养对转TNF—α基因鱼腥藻7120的生长有利.单独流加碳源对转基因鱼腥藻7120生长与外源基因表达影响很小,单独流加氮源和同时流加碳氮源可明显促进生长,生物量达到2.964g/L和3.059g/L,分别增长9.91％和12.71％,而且能轻微促进藻细胞中的TNF表达.     

二种鸟疟原虫红内期的超微结构的观察

通过对广西疟原虫新种（Plasmodiumguangxiensesp．nov．）、三趾鹑疟原虫新种（Plasmodiumturnixsp．nov．）的红内期有性体和无性体电镜观察，结果表明2种的超微结构与前人报导的已知种的超微结构基本一致．     

一株产生淀粉糖化酶的拟青霉

从土壤中分离筛选到一株降解生淀粉能力较强的菌株,通过固体发酵其生淀粉糖化酶活为2237U/g（按U/g麦麸计）,RDA值为15.25%。根据形态学特性初步鉴定该菌属于拟青霉属（Paecilomyces sp.）。其最适生长温度为30℃,最适生长起始pH5。其粗酶液24h水解生玉米淀粉的水解率为86.88%;对不同来源的生淀粉的水解能力不同,为玉米粉〉面粉〉米粉〉木薯粉〉甘薯淀粉;其最适作用pH为4,最适作用温度为70℃。     

紫外线复合Nd:YAG激光对Bacillus sp.产脂肪酶的诱变

以Bacillus sp．为出发菌株，经过紫外线诱变，筛选出较稳定的菌株UV-4，其产脂肪酶活力较出发菌株提高了28％；UV-4经过YAG激光诱变处理，得到变株Y-1，其产酶能力在此基础上又提高了41％，且产酶水平较为稳定。     

Isolation and characteristics of Arthrobacter sp. strain CW-1 for biodegradation of PAEs

Isolation of new bacterial strains and recognition of their metabolic activities are highly desirable for sustainability of natural ecosystems. Biodegradation of dimethyl phthalate (DMP) under anoxic conditions has been shown to occur as a series of sequential steps using strain CW-1 isolated from digested sludge of Sibao Wastewater Treatment Plant in Hangzhou,China. The microbial colony on LB medium was yellowish,3～5 mm in diameter,convex in the center,and embedded in mucous externally. The individual cells of strain CW-1 are irregular rods,measuring (0.6～0.7)×(0.9～1.0) μm,V-shaped,with clubbed ends,Gram positive and without any filaments. 16S rDNA (1438 bp) sequence analysis showed that the strain was related to Arthrobacter sp. CW-1 and can degrade PAEs utilizing nitrate as electron acceptor,but cannot mineralize DMP completely. The degradation pathway was recommended as: dimethyl phthalate (DMP)→monomethyl phthalate (MMP)→phthalic acid (PA). DMP biodeg-radation was a first order reaction with degradation rate constant of 0.3033 d-1 and half-life 2.25 d. The DMP conversion to PA by CW-1 could be described by using sequential kinetic model.     

一株新型稻瘟霉素链霉菌JZB130180的鉴定及其对桃褐腐病的生防效果评价（英文）

目的:筛选对桃褐腐病有显著防效的链霉菌并解析其生防因子。创新点:本研究筛选到一株新型稻瘟霉素链霉菌JZB130180,其对桃褐腐病具有显著防效。通过对其生防因子解析及发酵液分析,为后续分离抑菌次生代谢物奠定了基础。方法:通过对峙培养和离体实验,检测链霉菌对桃褐腐病的抑制作用。通过平皿抑菌实验,计算链霉菌发酵液的室内毒力;随后进行了发酵液的理化性质检测。通过酶活力测定等,解析链霉菌中的生防因子。结论:链霉菌JZB130180及其发酵液对桃褐腐病具有显著的抑制作用。发酵液对桃褐腐病菌的半数有效抑制浓度(EC50)为38.3μg/mL,且对酸碱、温度和紫外线都有一定的耐受能力。此外,该菌株可以分泌几丁质酶、纤维素酶、蛋白酶等重要生防因子。     

Spatio-temporal expression of the pathway-specific regulatory gene redD in S. coelicolor

INTRODUCTION Streptomycetes are gram-positive soil bacteriarenowned for their ability to produce a large numberof different secondary metabolites. Their life cycleinvolves complex morphological differentiation,which is believed to be closely co-ordinated with geneexpression that promotes a variety of physiologicaland structural changes including the onset of antibi-otic biosynthesis. S. coelicolor has been extensivelystudied as a model system with the aid of genetic andmolecular technique…