复方丹参片早期干预对APP/PS1转基因小鼠学习记忆的影响

目的：探讨复方丹参片早期干预对APP/PS1转基因小鼠学习记忆的影响及作用机制。方法：4月龄野生型小鼠和APP/PS1转基因小鼠分别灌胃给予溶剂或药物8周后,采用新物体识别实验和跳台实验评价复方丹参片对小鼠行为学表现的影响;应用ELISA检测小鼠海马β淀粉样蛋白（β-amyloid protein,Aβ）Aβ40、Aβ42的含量;采用Real time PCR和Western blot分别检测胰岛素分解酶（insulin degrading enzyme,IDE）/脑啡肽酶（neprilysin,NEP）m RNA和蛋白水平的变化。结果：新物体识别实验结果显示,复方丹参片720 mg·kg-1显著性增加APP/PS1转基因小鼠对新物体的识别指数。跳台实验结果显示,复方丹参片720、360 mg·kg-1显著性减少APP/PS1转基因小鼠学习次数,此外,复方丹参片720 mg·kg-1明显增加小鼠在平台的停留时间。ELISA结果显示,复方丹参片720、360 mg·kg-1显著性降低APP/PS1转基因小鼠海马Aβ40、Aβ42水平。Real-time PCR结果显示,复方丹参片720 mg·kg-1显著性上调APP/PS1转基因小鼠海马IDE和NEP m RNA的表达。Western blot结果显示,复方丹参片720 mg·kg-1显著性增加小鼠海马IDE蛋白表达。结论：复方丹参片可改善APP/PS1转基因小鼠学习记忆能力,可能与减少海马Aβ水平和增加IDE的表达有关。     

R&D co-operation and innovation a microeconometric study on French firms

The scope of this paper is to report new empirical evidence on the determinants of R&D co-operation. Indeed, the literature on the capabilities of firms emphasises the role of knowledge in the performance and evolution of firms who use knowledge developed in others to build their own knowledge capital. R&D co-operation between firms is one of the many strategies by which this knowledge may be transmitted. Several theoretical models have stressed that R&D co-operation is more likely when the level of spillovers is high. While this supposition is used in many theoretical models, it has rarely been tested before. Our results do not lend strong support to this intuition. Indeed, our spillover variables (national pure spillovers, national rent spillovers and imports of machine tools), which were supposed to match the theoretical notion of spillovers used in these theoretical models have a positive but not a significant role when explaining R&D co-operation. The R&D co-operation increases with size and with R&D intensity, but not with market share. It also increases with the budget spent on paying license fees and on acquiring patents and labour from foreign firms. In this study, we also analyse the determinants of innovation. In more precise terms, we compare the effects of R&D co-operation to those played by traditional internal factors and those exerted by external, pure and rent spillovers on the innovation capacity of the firms. Our results show that the commercial success of innovations of French firms depends mainly on size, market share, R&D intensity and human capital. Inward FDI from industrialised countries exerts a positive and significant effect. The finding highlights the important role of the absorptive capacity of firms. Spillover measurements, such as the acquisition of machine tools, foreign patents, licenses, and technological opportunities have a positive impact on innovation.     

标度选择对群决策影响的研究

人们在研究群决策问题的时候很少关注标度问题,本文通过实例说明标度对群决策的影响是至关重要的。同时通过对标度问题的研究,给出了评价标度合理性的准则。对人们普遍使用的标度进行了简单的评价,说明了指数标度的合理性。     

先秦汉语V_1而不V_2结构研究

先秦汉语中“V1而不V2 ”是一种常见结构形式 ,其变化格式有“动 动”、“动 形”、“形 形”、“名 名”等不同类型 ;V1与V2 之间的意义关系也多种多样 ,有同义关系、近义关系、反义关系等 ;其语法功能相当于一个词组 ,可作主语、谓语等句子成分     

学习者因素与母语正迁移

二语习得研究表明母语正迁移作用不容忽视。母语正迁移受到诸多因素的影响和制约,其中学习者因素在很大程度上对母语迁移的发挥起到决定性作用。影响和制约母语迁移的学习者因素主要有学习者的意识因素以及学习者的发展期因素等。在前人研究的基础上,探讨和研究学习者因素与母语正迁移的关系有利于对语言正迁移进行全面认识并提高对二语习得本质的理解并促进二语习得。     

高等职业教育实施“1+X”证书制度培养模式探析

“1+X”证书制度是关于职业教育人才培养模式、评价模式的一种制度设计,契合当今高等职业教育发展的要求,在增强职业教育适应社会经济发展能力,培养智能化时代复合型人才,指导未来职业教育,推进高职教育制度与就业制度改革,以及高等职业教育教学管理模式的变革等方面具有重要意义。该模式可以提升高等职业院校学生的就业竞争能力和院校的人才供给能力,增强职业院校的市场吸引力,为推动我国职业教育的深入改革提供持续动力。     

荧光PCR基因分析仪的快速并行温度控制

提出一种模仿人工经验的智能升降温控制系统,成功地应用于荧光PCR基因分析仪,同时也给出DS18B20温度传感器的详细用法及程序。     

HPLC-ELSD法测定血栓通注射液中人参皂苷Rg1和人参皂苷Rb1的含量

目的:用HPLC-ELSD法测定血栓通注射液中人参皂苷Rg1和人参皂苷Rb1的含量。方法:HPLC-ELSD法,色谱柱:WaterODS色谱柱(4.6x150mm,5um),流动相:乙腈-水(20:80-40:60)。结果:人参皂苷Rg1回收率为99.0%,RSD=1.02%;人参皂苷Rb1回收率为99.3%,RSD=1.31%。结论:本法简便、准确,可作为该制剂的质量控制方法。     

NPC蛋白研究进展

C型尼曼氏病(Niemann-Picktype C，NPC)是一种致命的神经退行性失调疾病，常见于儿童早期发作。其特征是患者细胞中胆固醇和鞘糖脂等脂类沉积在晚期胞内体／溶酶体中。NPC症状是由NPCI或NPC2的功能缺陷引起的。NPCI是一个跨膜蛋白，目前认为它参与内吞后的底物向高尔基复合体或胞内其它位点运输，在脂类物质循环和平衡控制机制中起重要作用。NPC2是一个可溶性溶酶体蛋白，可结合胆固醇。目前尚不清楚NPCI和NPC2如何调控溶酶体等细胞器的确切功能。