IN VITRO STUDY ON RIBOZYME AGAINST HEPATITIS C VIRUS

ＩＮＴＲＯＤＵＣＴＩＯＮＲｉｂｏｚｙｍｅｗａｓｆｉｒｓｔｄｅｓｃｒｉｂｅｄｂｙＣｅｃｈａｎｄＡｌｔｍａｎｉｎ 1 981 .ＴｈｉｓｆｏｒｍｏｆＲＮＡｈａｄｈｉｇｈｌｙｓｐｅｃｉｆｉｃｅｎｄｏｎｕｃｌｅａｓｅａｃｔｉｖｉｔｉｅｓｔｏＲＮＡ (Ｋｒｕｇｅｒｅｔａｌ.,1 982 ) .Ｒｉｂｏｚｙｍｅｓｈａｖｅｔｈｒｅｅｓｔｒｕｃｔｕｒｅｓ:ｈａｍｍｅｒｈｅａｄ ,ｈａｉｒｐｉｎａｎｄａｘｅｒｉｂｏｚｙｍｅｓ.Ａｍｏｎｇｔｈｅｍ ,ｔｈｅｈａｍｍｅｒｈｅａｄｒｉｂｏｚｙｍｅｈａｓａｓｉｍｐｌｅｒｓｅｃｏｎｄａｒｙｓｔｒｕｃ…     

丙型肝炎的免疫病理及临床防治研究进展

丙型肝炎(HC)发病与血源、体液的传播有关.HC的组织病理学改变表现为汇管区淋巴细胞聚集,淋巴滤泡形成,小胆管损伤,肝细胞大泡性脂肪变等.目前国内外以干扰素、核酸及核酶治疗为主.干扰素通过抑制HCV复制改善症状,减少肝细胞损害,减轻肝细胞炎症,降低ALT及TB,减慢向肝硬化、肝癌的演变而发挥重要作用.核酸治疗通过合适的载体将核酶导入细胞内,再在细胞内转录产生核酶从而发挥更稳定的抗病毒作用,其疗效也更为有效.     

关于冬虫夏草对乙型肝炎疗效及对免疫性肝损伤保护作用的研究

根据近10多年的研究报导及课题组的研究结果，综述了冬虫夏草（CS）对乙型肝炎的治疗效果及对免疫性肝损伤的保护作用。试验证明，乙型肝炎患者经CS治疗后，HbeAg有较高的阴转率，ALT、AST等肝功能指标明显改善，乏力、纳差等症状也大为好转，患者外周血中的CD3、CD4、CD4/CD8均明显提高。即便发生肝硬化者，经一定疗程CS治疗后，硬化症状减轻，肝纤维化得到有效控制和减轻。对免疫性肝损伤有很好的保护作用。     

Gene transfer into primary cultures of fetal neural stem cells by a recombinant adenovirus carrying the gene for green fluorescent protein

Objective: To evaluate the transduction efficiency of a recombinant adenovirus carrying the gene for green fluorescent protein (Ad-GFP) into the primary cultures of fetal neural stem cells (NSCs) by the expression of GFP. Methods: The Ad-GFP was constructed by homologous recombination in bacteria with the AdEasy system; NSCs were isolated from rat fetal hippocampus and cultured as neurosphere suspensions. After infection with the recombinant Ad-GFP, NSCs were examined with a fluorescent microscopy and a flow cytometry for their expression of GFP. Results: After the viral infection, flow cytometry analysis revealed that the percentage of GFP-positive cells was as high as 97.05%. The infected NSCs sustained the GFP expression for above 4 weeks. After differentiated into astrocytes or neurons, they continued to express GFP efficiently. Conclusion: We have successfully constructed a viral vector Ad-GFP that can efficiently infect the primary NSCs. The reporter gene was showed fully and sustained expression in the infected cells as well as their differentiated progenies.     

横梁镇居民乙肝健康教育的效果分析

目的 了解对横梁镇居民进行乙肝防治知识知晓情况和相关态度,评价开展农村居民乙肝健康教育的效果.方法 对451名居民进行乙肝防治知识问卷调查,内容包括乙肝的基本情况、乙肝传播途径及预防措施、对乙肝患者的态度等.结果 关于乙肝传播途径及乙肝预防措施的认识调查后知晓率提高1.6%-17.5%.对乙肝一般情况及对乙肝患者的态度教育后8个问题知晓率提高2%-31%.结论 对农村居民开展乙肝防治知识健康教育取得较好的效果,但完全认知率不高,应进行经常性教育.     

一步之遥，八年光阴：丙肝病原体的发现到证明之路

2020年的诺贝尔生理学或医学奖授予了在发现丙肝病毒(HCV)方面作出突出贡献的哈维·阿尔特(Harvey J. Alter)、迈克尔·霍顿(Michael Houghton)和查尔斯·赖斯(Charles M. Rice)。然而,从1989年霍顿捕获HCV到1997年赖斯证明HCV能引发肝炎,间隔了8年时间。这看似一步之遥的距离何以耗时如此之久?文章通过回顾文献,对这一时期的研究脉络进行了梳理,明确了包括赖斯团队在内的不同研究者对于推进HCV相关认识所作的具体贡献。同时,针对研究过程中的两个关键节点——病毒RNA基因组3′末端的准确测序和感染性分子克隆的建立,文章从技术储备、路径依赖和目标定位的角度分析了赖斯团队为何能在科学研究竞争中拔得头筹。     

苦参素联合熊去氧胆酸对慢性乙型肝炎GGT的疗效观察

目的探讨苦参素联合熊去氧胆酸(UDCA)治疗GGT持续升高的慢乙肝患者的疗效.方法治疗组47例给予UDCA联合苦参素治疗,对照组39例只用苦参素治疗,疗程2周.观察治疗组与对照组血清GGT值的变化.结果慢乙肝中度患者中,治疗组与对照组治疗后GGT值相比有明显差异(P＜0.05).结论应用UDCA和苦参素治疗慢性乙型肝炎能有效降低GGT,且无明显副作用.     

慢性乙型肝炎病毒感染学生体育锻炼的现状

运用问卷调查法对高校慢性乙肝感染学生的体育锻炼意识、目的、行为和技能进行了调查分析,结果表明,慢性乙肝病毒感染学生担心体育锻炼损伤身体,体育锻炼意识及达标率明显低于正常学生,体育锻炼目的主要是应付考试。有必要对这些学生加强卫生宣传教育,改革教学方法。     

Hepatitis during respiratory syncytial virus infection – a case report

Introduction:

Respiratory syncytial virus (RSV) infection is the most common cause of hospitalization in infants and small children. The aim was to present a 13-months old boy diagnosed with acute airway infection, acute otitis media (AOM) and hepatitis during the RSV-infection.

Material and methods:

Serum catalytic activities of alkaline phosphatase (ALP), aspartate aminotranspherase (AST), alanine aminotranspherase (ALT), gamma glutamyl transpherase (GGT), lactate dehydrogenase (LD), and concentrations of bilirubin were monitored during hospitalization and at control examination.

Results:

The child had clinical signs and symptoms of respiratory failure, AOM, and laboratory findings of virus infection and liver disease. On admission, catalytic activities of enzymes were markedly increased, especially the activity of ALP (10333 U/L, i.e. 24-fold increase in comparison with the upper reference limit). The highest increased in AST (339 U/L, 4.5-fold), ALT (475 U/L, 10.3-fold) and LD (545 U/L, 1.5-fold) were registered on the 3^rd day, and the highest increase in GGT (68 U/L, 3.1-fold) occurred on the 11th day. Seven weeks after discharge AST, ALT, GGT and LD decreased into reference range, and ALP remain mildly increased (478 U/L, 1.1 fold increase). RSV was confirmed in nasal lavage fluid.

Conclusion:

Laboratory results in patient with RSV infection needs to be interpreted in the light of both, respiratory and extrapulmonary manifestations of the infection, respectively.     

网络企业病毒式营销策略分析

随着互联网的快速发展,其应用价值得到众多企业的认可,越来越多企业选择在互联网环境下开展营销活动。作为网络营销方式之一,病毒式营销应用价值日益显现。本文在此背景下,首先介绍了病毒式营销的概念和要素,其次对几组典型的网络企业病毒式营销应用实例进行分析,最后提出网络企业实施病毒式营销的几点策略。