毛细管电泳安培检测法测定山楂中的有效成分

用毛细管电泳安培检测法同时测定山楂中的芦丁、金丝桃甙和绿原酸的含量.考察了实验条件包括电极电位、运行缓冲液的酸度和浓度、电泳电压及进样时间对三组分分离和检测的影响.在实验条件为：以碳圆盘电极（直径300μm）为检测电极,检测电位为＋0.95 V（vs.SCE）,在50 mmoL/L硼酸盐（pH为9.2）的运行缓冲液中,芦丁、金丝桃甙和绿原酸在20 min内能完全分离.被测物浓度与响应电流在三个数量级范围内呈较好的线性,检测限分别为1×10-8、1×10-8和2×10-8g/mL.     

DP-AW表面张力组合实验仪的使用与改进

根据DP-AW表面张力组合实验仪使用中的问题,提出了对部分组件的改进意见及仪器使用中的一些注意事项。     

大口径石英毛细管气相色谱法测定盐酸尼非卡兰中有机残留溶剂

通过外标定量法 ,利用大口径石英毛细管柱测定盐酸尼非卡兰中有机溶剂甲醇、氯仿、吡啶、四氢呋喃和二甲基甲酰胺的残留量 .该方法的相关系数均大于 0 .99,相对标准偏差小于 7.0 % ,最小检出量 :甲醇为 1× 10 -3 μg ;氯仿为 2× 10 - 3μg ;吡啶为 5× 10 -3 μg;四氢呋喃为 2× 10 -3 μg ;二甲基甲酰胺为 8× 10 -3 μg.     

Gamma heavy chain disease in a patient with rheumatoid arthritis – a laboratory evaluation

Introduction:

Heavy chain diseases (HCD) are neoplastic proliferations of B cells which secrete truncated immunoglobulin heavy chains without associated light chains. Being rare and probably underdiagnosed diseases the aim of this report is to show an additional case of gamma heavy chain disease in a 48 year old female patient with rheumatoid arthritis focusing on the laboratory presentation.

Materials and methods:

Laboratory work-up included agarose gel electrophoresis (AGE), capillary zone electrophoresis (CZE), immunofixation and nephelometrically determined immunoglobulin and immunoglobulin subclasses of the patient’s serum. Urine samples were also subjected to immunofixation and to a SDS-PAGE with consecutive immunoblot.

Results:

Nephelometrically measured elevated IgG concentrations were noted in combination with a decreased gamma globulin region and an increased beta globulin region on AGE. A definite monoclonal spike was not identified on AGE but at least suspected on CZE; finally serum and urine immunofixation demonstrated a monoclonal gamma heavy chain devoid of any corresponding light chains confirming the diagnosis of HCD. Analysis of the gamma heavy chain (HC) with means of SDS-PAGE revealed proteins of 40 kD and 80 kD most likely presenting a truncated HC in its monomeric and dimeric form and possibly leading to the failure of IgG-subclass typing with the applied IgG subclass antisera.

Conclusion:

This case report illustrates a new case of gamma HCD demonstrating variable laboratory manifestations and therefore the need for heightened awareness concerning this disease when confronted with abnormal and discrepant protein profiles in routinely applied laboratory tests.     

不同耐力负荷对左室乳头肌纤维及其血管变化的实验研究

本文以不同耐力负荷的雄性 Wistar 大鼠心脏为研究对象,采用活体腹主动脉注墨,组织切片,HE、Masson 染色等方法,观察了不同耐力负荷下左室乳头肌纤维及其血管的变化规律。     

毛细管电泳中固定套管式电极定位装置的改进

研究采用固定套管式电极调节定位方式,实现了工作电极与毛细管检测端口的完美对接。将两石英玻璃套管(内径400μm)经精确准直后固定在检测池中,工作电极由细微的石英玻璃毛细管(外径375μm,内径200μm)制作而成,工作时只需将制作的工作电极和电泳毛细管(石英玻璃质,外径375μm)分别插入套管,在两套管(间距约3 mm)中部轻微接触对接即可。该方法定位精确,操作简单方便,工作效率高,检测重现性好,较目前市面上部分使用的三维自由调节方式和螺杆调节方式的检测池有明显的优势和实用意义。     

Characterization and separation of DNA complexes containing poly-L-lysine with or without the modification of PEG by capillary electrophoresis

The characterization of complexes is particularly critical for quality control and development of gene delivery systems. Here, the method of capillary zone electrophoresis (CZE) for the characterization of DNA and poly-L-lysine (MW 28 500) or DNA and poly-L-lysine modified with polyethylene glycol (MW10000) complexes at various charge ratios in phos-phate buffer is described firstly. During the characterization, DNA complexes can be separated into various components with different charge-to-mass ratio, i.e, components with single physicochemical property. And also the size and zeta potential of complexes were characterized by using photon correlation spectroscopy. This method is useful to characterize various com-plexes formed by DNA and polycations, and has the potential to separate complexes into homogeneous component for better transfection efficiency in vitro and in vivo in future.     

毛细管等速电泳检测器

对毛细管等速电泳检测器进行了分类，扼要地评述了各种类型检测器的结构、原理和性能，并展望了检测器的发展前景．     

RAPID DETERMINATION OF GUANINE BY HIGH PERFORMANCE CAPILLARY ELECTROPHORESIS

A simple and reproduciable method for determining guanine by high performance capillary electrophoresis is presented. The method involved the use of 2-amino-5-mercapto-3,4-dithiazole as internal standard and 40mmol/L borax-H3PO4 (pH2)-5% EtOH as.the background electrolyte. The result showed that the linear range for guanine is 5-300 mg/L with the recovery of 94%-98% , the detection limit of 2. 80 mg/L and the RSD of 3. 1%. The satisfactory result was obtained when it was used to detect the concentration of guanine in acyclovir product.     

毛细管电泳法研究槐角生长过程中黄酮类化合物含量变化

建立了一种毛细管电泳法测定槐角中黄酮类化合物的方法,利用所建立的方法研究了槐角生长过程中黄酮类化合物的含量变化,所得结果对槐角药物质量控制和药用资源开发具有一定参考价值．