顶空^[4]气相色谱法测定头孢他啶中的残留溶剂——丙酮、二氯甲烷、乙酸乙酯

目的：建立一种气相色谱法同时测定头孢他啶中丙酮、二氯甲烷、乙酸乙酯有机溶剂残留量的。方法：采用外标法顶空自动进样,在非极性弹性石英毛细柱^[1]上进行各组分分离,效果良好。结果：平均回收率丙酮98．6％～102．3％;二氯甲烷99．3％～102．6％;乙酸乙酯97．6％～99．5％;变异系数丙酮2．6％;二氯甲烷2．5％;乙酸乙酯3．2％。结论：方法重现性好,定量准确,便于操作。     

Gamma heavy chain disease in a patient with rheumatoid arthritis – a laboratory evaluation

Introduction:

Heavy chain diseases (HCD) are neoplastic proliferations of B cells which secrete truncated immunoglobulin heavy chains without associated light chains. Being rare and probably underdiagnosed diseases the aim of this report is to show an additional case of gamma heavy chain disease in a 48 year old female patient with rheumatoid arthritis focusing on the laboratory presentation.

Materials and methods:

Laboratory work-up included agarose gel electrophoresis (AGE), capillary zone electrophoresis (CZE), immunofixation and nephelometrically determined immunoglobulin and immunoglobulin subclasses of the patient’s serum. Urine samples were also subjected to immunofixation and to a SDS-PAGE with consecutive immunoblot.

Results:

Nephelometrically measured elevated IgG concentrations were noted in combination with a decreased gamma globulin region and an increased beta globulin region on AGE. A definite monoclonal spike was not identified on AGE but at least suspected on CZE; finally serum and urine immunofixation demonstrated a monoclonal gamma heavy chain devoid of any corresponding light chains confirming the diagnosis of HCD. Analysis of the gamma heavy chain (HC) with means of SDS-PAGE revealed proteins of 40 kD and 80 kD most likely presenting a truncated HC in its monomeric and dimeric form and possibly leading to the failure of IgG-subclass typing with the applied IgG subclass antisera.

Conclusion:

This case report illustrates a new case of gamma HCD demonstrating variable laboratory manifestations and therefore the need for heightened awareness concerning this disease when confronted with abnormal and discrepant protein profiles in routinely applied laboratory tests.     

抗坏血酸的毛细管电泳-柱端铁氰化镍修饰镍微电极电化学检测

以K3Fe(CN)6溶液为电解液,在电沉积Ni表面形成铁氰化镍膜,制得了铁氰化镍修饰Ni电极,研究了修饰电极的电化学性质及其对抗坏血酸的电催化氧化作用。该修饰电极用于抗坏血酸的毛细管电泳-电化学检测,检测的线性浓度范围为2.0×10-6~5.0×10-3mol·L-1,检测限为5.0×10-7mol·L-1。     

微波辐射固定对内源性过氧化物酶的影响——显示微血管方法的研究

为了观察不同固定液和不同条件下对内源性过氧化物酶活性的影响,将大鼠脑、脊髓及猪皮肤置于六种固定液内,分别进行室温微波辐射、低温微波辐射固定和单纯浸泡固定,结果表明,低温微波辐射固定时,内源性过氧化物酶活性最强,显示血管数量最多,颜色最深。文中对微波辐射固定的优点及原理进行了讨论.     

毛细管气相色谱法定量测定深海鱼精油中的EPA．DHA

内标与外标相结合定量,毛细管柱气相色谱分析测定深海鱼精油中EPA．DHA的含量回收率为99．7％－100．51％。变异系数为2．49％－3．65％。     

毛细管区带电泳分离测定肾上腺素和去甲肾上腺素

利用高效毛细管区带电泳研究了人血清中肾上腺素和去甲肾上腺素的分离与测定。考察了背景缓冲溶液及其添加剂、分离电压、进样时间等对分离测定肾上腺素和去甲肾上腺素的影响，确定了最佳分离条件。血清样品经过简单处理。蛋白质不干扰测定。以多巴胺为内标，测得血清中的肾上腺素和去甲肾上腺素的浓度在1—30mg／L范围内均呈良好线性关系，相关系数分别为0．9998和0．9994。检测限分别为0．1mg／L和0．3mg／L。该方法具有快速、简便、重现性好等优点。     

毛细管等速电泳检测器

对毛细管等速电泳检测器进行了分类，扼要地评述了各种类型检测器的结构、原理和性能，并展望了检测器的发展前景．     

毛细管气相色谱法测定食用植物油中主要脂肪酸的相对含量

用毛细管气相色谱法测定了食用植物油中主要脂肪酸的相对含量。     

毛细管电泳在环境分析中的应用

毛细管电泳(Capillary Electrophoresis)是以毛细管为分离通道,高压电场为驱动力的快速高效的分离分析手段.适应环境分析中多组分混合、微痕量存在和连续监测等的分析要求,毛细管电泳技术在环境分析中表现出良好的应用前景.     

为生物学专业学生开设毛细管电泳开放实验的探索与实践

该文介绍了为生物化工和生物技术二，三，四跨年级本科生共同开设“毛细管电泳开放实验”的选题目的和意义。通过开放实验，学生普遍提高了查阅文献、设计实验内容、选择实验方案、解决实验问题、分析实验结果的能力，并深入学习和了解了毛细管电泳这一新型分析技术及其在具体科研和生产过程中的应用。