影响聚式酶链式反应实验效果的基本因素

研究聚式酶链式反应(PCR)实验中影响其扩增效果的多种因素,寻求反应体系中理想的各因素浓度配比。选取含5+10优质亚基的小麦品种为材料,以特异扩增1DX5基因PCR实验为例,从模板DNA、引物终浓度、Taq酶、二价镁离子、4dNTPs混合液、缓冲液浓度几方面入手,通过设计梯度实验,研究不同因素对PCR扩增效果的影响。结果表明,反应体系6个基本因素,缺少任何1个都扩增不出产物。在反应总体积25μL的PCR反应体系中,模板DNA选用32 ng/μL,Mg2+终浓度2 mmol/L,Taq酶1.2 U/25μL,引物0.8μmol/μL,4dNTPs0.3 mmol/L,缓冲液1×buffer最为合适,能扩增出理想的结果。     

基于THPZKZ-1型中央空调系统温度控制实验的研究

首先,介绍THPZKZ-1型中央空调系统的实验平台。然后,以此平台为基础,设计单回路温度控制实验,通过临界比例度法整定PID参数。最后,分析实验结果,总结控制规律。     

The Art of Straying: Benjamin on Distraction and the Informal Education of the City

In this article, Tyson E. Lewis argues for an alternative form of educational attunement beyond attentiveness. Discourses and practices of schooling emphasize being attentive as a primary educational virtue. This emphasis casts distraction as either the negation of attention or its deterioration. In either case, distraction lacks educational use and, as such, must be overcome through the cultivation of attention. Yet this approach to the question of attention versus distraction misses how distraction contains within itself a different kind of educational potentiality, a different kind of educational attunement. In an attempt to carve out space and time for distraction, Lewis turns to the writings of Walter Benjamin who offers up a more complex view of distraction than is presently found in educational philosophy. In particular, Benjamin argues that cities are not only places of negative forms of distraction (as mere diversions), but also places for cultivating productive distraction in the form of an open and alert attunement to new ways of experiencing and perceiving the relationship between world and self. Cities, for Benjamin, become educational spaces that teach lessons in a distracted form of life, the culmination of which is the creativity expressed in what he refers to as “the art of straying.”     

Calculated democracy—Explorations in gerrymandering

This paper describes a lesson centered around a metric for measuring partisan gerrymandering: the efficiency gap. We situate the efficiency gap in the current US political climate, describe the activity itself, and then provide recommendations for readers interested in adapting the lesson for their own classrooms.     

Critical Thinking and Community Engagement through Artistic Actions

In a society that strives for sustainability it is important to observe and value different forms of knowledge. Creating art is not a meaningless exercise. Instead, one might say that works of art serve as a window that interprets the world. In the article it is argued that artistic actions can offer a combination of critical and creative thinking reflecting on the types of knowledge and experience that count as valuable for sustainable communities. The article reflects on the participatory installation Colours of Rovaniemi and how ABER was used in the creation process. The methodology focused on the transformative power of community engagement when creating artistic actions that engage with sustainability. The installation provoked critical thinking and encouraged the participants to take a stand on the issues discussed. Artworks of this kind are well suited to help us understand the issues we face regarding sustainability. The work raised questions of who we are, what we prioritise in our lives. It initiated discussion of the connection between man and nature, attitudes, values and national self‐identity, challenging the participants’ perspectives.     

高校文科非哲学专业逻辑学教学改革新探

逻辑学教学改革由来已久,但始终没有达成共识,＂传统＂与＂现代＂之争还在继续,但或许此时我们该重新审视此争论并提出新的改革方案。为此,在已有教学实践的基础上,对高校文科非哲学专业逻辑学教学从教学目标、学生需求、学生可接受程度三个方面进行分析,提出了将形式逻辑与非形式逻辑（批判性思维）结合在逻辑学这一课程名称之下的改革方案,并具体分析了改革后逻辑学课程设置、教学内容的选择、教学方法的运用、教师队伍建设、教材编写几个方面的内容。     

浅述碱-骨料反应对混凝土耐久性的影响及预防措施

碱骨料反应对混凝土耐久性有极大的危害,本文就碱骨料反应的机理、产生条件、破坏特征、骨料碱活性的测定和如何预防进行了阐述。     

四组分反应高效合成多取代环庚基吡啶衍生物

在微波加热条件下,芳香醛、环庚酮、溴代对苯基苯乙酮吡啶盐和醋酸铵在醋酸中反应,以中等产率快速合成多取代环庚基吡啶衍生物。产物结构经过光谱方法和X-衍射单晶结构分析确证。本研究采用一锅四组分反应的方法,是高效合成多取代环庚基吡啶衍生物的新方法。     

教师发展阶段理论综述及发展水平模型的构建

一、研究源起 教师的专业成熟要经过一个长期的发展过程,从一个新教师成长为一个合格的、优秀的教师乃至知名的教育家,不是一蹴而就的,就专业成熟的过程而言,需要经历一个发展阶段。因此,对教师成长的周期和规律的研究,有助于为教师指明个人的专业发展道路,为教师的专业学习和训练提供需要的基本内容。     

向死而生的狂欢——对杨昭中篇小说《日蚀》的深度解读

以《日蚀》为标本,解析杨昭小说写作思想的根源,透视"新现实主义"在新世纪文坛视域下的拓展维度,从而再次观照中国继鲁迅以来对国民性探索的当下性创作。