固定在脂质双层膜内的细胞色素c氧化酶电化学研究

含有单体牛细胞色素c氧化酶的脂质双层膜，被成功地固定在金石英晶体微平衡电极上．在较宽的pH值、温度和缓冲浓度范围内观察到了氧化酶修饰电极上的直接电子迁移．氧化酶修饰电极在80℃以上时保持直接电子迁移性质。将电极冷却到室温时，氧化酶仍保持电子迁移能力．在22～80℃范围内。温度变化可引起细胞色素c氧化酶的相转移，估计出了相转移前后的相应反应活化能通过射流分析，研究了在溶液含有亚铁细胞色素c氧化酶的电氧化过程中。乙腈同细胞色素c氧化酶的结合．结果显示：浓度1．3M具有常数Ki时。细胞色素c氧化酶和乙腈形成配合物．每个细胞色素c氧化酶配一个乙腈分子。乙腈同细胞色素氧化酶的结合过程是可逆过程．     

Rechtliche Rahmenbedingungen für die Gestaltung universit?rer Curricula

Die bereits im Zuge der Autonomie-St?rkung der Universit?ten durch das UOG 1993 im Rahmen der Erlassung des UniStG 1997 vorgenommene Aufwertung der Selbstregelungskompetenzen der Universit?ten bei der Gestaltung des Studienrechts, findet in der Fassung der studienrechtlichen Vorgaben des UnivG 2002 seine konsequente Fortsetzung. Diese Ausweitung der Selbstregelungskompetenzen geht im rechtsstaatlichen Sinne typischerweise (als Gegengewicht) mit besonderen Anforderungen an Rechtsschutz und Rechtssicherheit einher. Insofern hat der universit?re Verordnungsgeber auch auf die in den Gesetzesgrundlagen zum Ausdruck kommenden Wertungen und Grunds?tze eingehend Bedacht zu nehmen.     

无线局域网安全问题及解决方案

本研究了无线局域网常用的安全技术，分析了IEEE802．11b存在的主要安全缺陷，提出了一些解决无线安全问题的方案。     

无线网络技术与校园网的组建

介绍无线网络的基本概念,阐述其技术特点、网络标准、应用领域及所需设备,并给出一个组建校园网络的方案设想.     

鸟类mtDNA中cytb基因及在系统进化研究中的应用

本文着重介绍了鸟类mtDNA中cytb基因的结构、功能、特点及其在鸟类系统进化研究领域中的主要应用。     

浅谈无线网络在图书馆的应用

建设一个高质量的无线局域网是目前大多数图书馆发展所必要的,在技术上也是完全可行的。构建无线网络包括两个部分:布线施工和认证系统规划,须遵循可靠、先进、实用、灵活扩展、易用、安全等原则。     

Determination of organophosphorus pesticide residues in vegetables by an enzyme inhibition method using α-naphthyl acetate esterase extracted from wheat flour

The widespread use of organophosphorus pesticides (OPs) poses a great threat to human health and has made the detection of OP residues in food an important task, especially in view of the fact that easy and rapid detection methods are needed. Because OPs have inhibitory effects on the activity of α-naphthyl acetate esterase (ANAE) in plants, in this work we evaluated the possibility of detecting OPs in vegetables with ANAE extracted from commercial flour. The limits of detection (LODs) obtained for methamidophos, dichlorvos, phoxim, dimethoate, and malathion in lettuce samples with crude ANAE were 0.17, 0.11, 0.11, 0.96, and 1.70 mg/kg, respectively. Based on the maximum residue limits (MRLs) for OPs in food stipulated by Chinese laws which are 0.05, 0.20, 0.05, 1.00, and 8.00 mg/kg for methamidophos, dichlorvos, phoxim, dimethoate, and malathion, respectively, the esterase inhibition method with crude ANAE had sufficient sensitivity to detect the residues of dichlorvos, dimethoate, and malathion in lettuce, but it could not be used to guarantee the safety of the same samples if methamidophos or phoxim residue was present. The sensitivity of the method was improved by the use of esterase purified by ammonium sulfate salting-out. The LODs obtained for methamidophos and phoxim with purified esterase were lower than the MRLs for these OPs in food. This is a very promising method for the detection of OP residues in vegetables using crude or purified esterase because of its cheapness, sensitivity, and convenience.     

Antitumor effects of human interferon-alpha 2b secreted by recombinant bacillus Calmette-Guérin vaccine on bladder cancer cells

Objective:Our objective was to construct a recombinant bacillus Calmette-Guérin vaccine(rBCG) that secretes human interferon-alpha 2b(IFNα-2b) and to study its immunogenicity and in vitro antitumor activity against human bladder cancer cell lines T24 and T5637.Methods:The signal sequence BCG Ag85B and the gene IFNα-2b were amplified from the genome of BCG and human peripheral blood,respectively,by polymerase chain reaction(PCR).The two genes were cloned in Escherichia coli-BCG shuttle-vector pMV261 to obtain a new recombinant plasmid pMV261-Ag85B-IFNα-2b.BCG was transformed with the recombinant plasmid by electroporation and designated rBCG-IFNα-2b.Mononuclear cells were isolated from human peripheral blood(PBMCs) and stimulated with rBCG-IFNα-2b or wild type BCG for 3 d,and then cultured with human bladder cancer cell lines T24 and T5637.Their cytotoxicities were measured by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide(MTT) assay.Results:BCG was successfully transformed with the recombinant plasmid pMV261-Ag85B-IFNα-2b by electroporation and the recombinant BCG(rBCG-IFNα-2b) was capable of synthesizing and secreting cytokine IFNα-2b.PBMC proliferation was enhanced significantly by rBCG-IFNα-2b,and the cytotoxicity of PBMCs stimulated by rBCG-IFNα-2b to T24 and T5627 was significantly stronger in comparison to wild type BCG.Conclusions:A recombinant BCG,secreting human IFNα-2b(rBCG-IFNα-2b),was constructed successfully and was superior to control wild type BCG in inducing immune responses and enhancing cytotoxicity to human bladder cancer cell lines T24 and T5637.This suggests that rBCG-IFNα-2b could be a promising agent for bladder cancer patients in terms of possible reductions in both clinical dosage and side effects of BCG immunotherapy.     

某类解析函数的系数泛函

引入了两个新的解析函数类μa,b（φ）和μa,b^λ（φ）,利用复分析中的一些方法,讨论了这两个函数Fekete—SzegO的不等式,得到了准确的结果,推广了一些作者的相关结果．     

关于R可积函数空间的完备化

完备性是度量空间中的一个重要性质,本文运用了实变函数中点集分析的方法及其相关定义和定理讨论了Riemann积分与Lebesgue积分的本质区别在于:R[a,b]作为L[a,b]的子空间是不完备的,而L[a,b]是完备的,并证明了R[a,b]在L[a,b]中稠密,最后得到了L[a,b]是R[a,b]的完备化空间.