SINGLE CELL DEGENERATE OLIGONUCLEOTIDE PRIMER-PCR AND COMPARATIVE GENOMIC HYBRIDIZATION WITH MODIFIED CONTROL REFERENCE

ＩＮＴＲＯＤＵＣＴＩＯＮＦｌｕｏｒｅｓｃｅｎｃｅｉｎ ｓｉｔｕｈｙｂｒｉｄｉｚａｔｉｏｎ (ＦＩＳＨ )ａｎｄｐｏｌｙｍｅｒａｓｅｃｈａｉｎｒｅａｃｔｉｏｎ (ＰＣＲ)ｈａｄｂｅｅｎｓｕｃｃｅｓｓｆｕｌｌｙｕｓｅｄｆｏｒｐｒｅｉｍｐｌａｎｔａｔｉｏｎｇｅｎｅｎｔｉｃｄｉ ａｇｎｏｓｉｓ (ＰＧＤ ) (Ｖｅｒｌｉｎｓｋｙｅｔａｌ.,1 997,Ｓａｂｔａｌｏｅｔａｌ.1 995)ａｌｔｈｏｕｇｈｔｈｅｙｎｏｒｍａｌｌｙｏｎｌｙｏｆｆｅｒｉｎｆｏｒｍａｔｉｏｎｆｒｏｍｏｎｅｏｒｆｅｗｃｈｒｏｍｏｓｏｍｅｒｅ ｇｉｏｎｓｏｒｓｐ…     

Hlder continuity for solutions of elltptic equations involving measures

1IntroductionLetGbeaboundeddomaininthen-dimensionalEuclideanspaceE".ItwasshownbylJ'M'RakotosonandW'P'Ziemerint13'thattheellipticequationinvolvingmeasurep-dit)A(x,u,7u) B(x,u,7u)=p,inG(l.1)isdeducedbysolvingvariationalinequalityinvolvingsameellipticdifferentialoperator.WenowconsiderthecasethatA(x,u,E)andB(x,u,E)sat1sfythefollowingrnoregeneralstructural(excepttheyaredefinedonGXE1XE",measurableinxandcontinuousinuandE)wherein(l.2)andthereexistconstantsL>O,0rA相似文献   

Single cell degenerate oligonucleotide primer-PCR and comparative genomic hybridization with modified control reference

For investigating the possibility of applying degenerate oligonucleotide primer PCR (DOP-PCR) and comparative genomic hybridization (CGH) technique to analyses of genomic genetics in a single cell, the whole genomic DNA of a single cell with XX, XY, XO, XXY, +13 or +21 was amplified by DOP-PCR. Single cell DOP-PCR CGHs with conventional and modified control references, the genomic DNA and a single cell DOP-PCR product from normal male, were carried out respectively. The results showed that the average profile of the fluorescence intensity ratio in CGH with the genomic DNA as reference fluctuates much and that the standard deviation in about 30% haploid is beyond the normal limits. False positive hyper-representation was found to exist in X chromosome while trisomy 13 and 21 were not detected. However, the distributions of the mean and the standard deviation of the ratio in the CGH with DOP-PCR product as reference were quite acceptable. The copy number changes of chromosome X, Y, 13 and 21 were revealed. Those results suggested that there is unrandom unequal amplification in a single cell DOP-PCR. Using a single DOP-PCR product as reference can decrease its influence on CGH. Single cell DOP-PCR-CGH and its application in the genetic analyses of preimplantation embryo or fetal cell in maternal blood may be possible.     

一类退化椭圆系统的线性特征值问题

通过对一类退化椭圆系统线性特征值问题的研究,得到了其线性特征值问题的一列无界的递增的特征值序列。同时,还给出了特征值序列以及对应的特征函数序列的若干性质,这为进一步研究退化椭圆系统的近共振问题奠定了理论基础。     

用筒并微扰法分析金属晶体中散射波较强情况

在一维晶格中,若电子波矢忌落在布里渊区边界k=±（nπ／α）时,电子将遭受到与布里渊区边界平行的晶面族的反射,由于在布里渊区边界散射波很强,满足布拉格全彦射的条件,此时微扰论不再适用,须用简并微扰法来处理电子的能带分布。     

以圆为变型线的二阶混合—复合型方程组的边值问题

使用极值原理,Fredholm二择一定理和压缩映射定理,证明二类边值问题解的存在性和第一类边值问题解的唯一性.     

二阶线性退化抛物方程的局部相容性

局部相容性条件在二阶线性退化抛物方程C∞光滑解的研究中起着相当重要的作用。本文证明了一类相当普遍的函数关于二阶线性退化抛物算子：满足局部相容性条件。     

双平均亚洲期权定价问题的Legendre谱方法

Abstract In this paper, the evaluation of discretely sampled Asian options was considered by numerically solving the associated partial differential equations with the Legendre spectral method. Double average options were discussed as examples. The problem is a parabolic one on a finite domain whose equation degenerates into ordinary differential equations on the boundaries. A fully discrete scheme was established by     

“同声”的失语与“指路石”的错位——从书评的现状看文坛的堕落

书评应是"读者的良友"与"作者的诤友",但当前良莠不齐、泥沙俱下的书评状况令人堪忧:一是纯粹商业功利化的广告式写作;二是为私谊无原则地对作品极尽拔高之能;三是沦为泄私愤相互公讦的口水。因而书评不仅丧失了服务于读者的信息功能、中介功能、导读功能等,而且在客观上也为文坛的堕落推波助澜。这不能不引起我们足够的重视。