Determination of Biological Variance and Validation of a Fluorometric Assay for Measurement of α-l-Iduronidase Activity in Dried Blood Spots Samples: The First Experience in Iran

Methods for assaying lysosomal diseases in dried blood samples are very useful today due to its several advantages related to the stability of samples, its transportation, handled and analysis, and its potential use for newborn screening compared to traditional methods in leucocytes samples. For this reason, it is important to validate these assays before being used in routine laboratory. Because of different in biological markers based on ethnicity, we aimed this study to validation a DBS-based fluorometric assay for measurement of α-l-Iduronidase activity for diagnosis of MPS I patients in Iran. DBS samples were collected from 15 MPS I patients and 60 healthy age matched subjects. Diagnostic value, biological variance and α-l-Iduronidase activity were determined. DBS α-l-Iduronidase activity was significantly higher in male subjects than in female group. Using a cut-off level of 1.08 µmol/spot 20 h, sensitivity and specificity were 100 and 98 %. The linearity of test was proved and we showed that within-run and between run precision were 5.6 and 14.66 %. Measurement of α-l-Iduronidase activity in DBS samples is an accurate test for diagnosis of MPS I and because of its rapid shipping and simplicity to keeping, DBS-based enzyme activity could be considered as a useful diagnostic tool in this disease.     

Self-report vs. kinematic screening test: prevalence,demographics, and sports biography of yips-affected golfers

The yips is considered a task-specific movement disorder. Its estimated prevalence, however, is high compared to similar neurological movement disorders, possibly resulting from previous studies’ restriction of samples based on skill level, and self-report bias. Alternatively, this high prevalence might be an indication of additional aetiologies, for example the influence of previously played racket sports. We estimated the prevalence of the putting yips across the skill range, using self-reports in one study and a screening test in a second study. We explored if previously played sports matter for the development of the yips. In study 1, yips prevalence (N = 1,306) and golfers’ sports biographies (n = 264) were examined via two online surveys, in which golfers indicated if they were yips-affected. In study 2, golfers (N = 186) putted in a standardised putting test while kinematic and performance measures were recorded. Prevalence was estimated via a kinematic threshold. Sports biographies (n = 119) were obtained via an online survey. Prevalence of currently yips-affected golfers was 22.4% in study 1 and 16.7% in study 2. In both studies, more yips-affected than unaffected golfers had experience in playing racket sports. Yips prevalence remained higher than previously estimated prevalence of other movement disorders but decreased when the whole skill range including professionals and novices was considered. Future studies should use the kinematic screening test instead of self-reports to detect the yips and further investigate the influence of previously played racket sports.     

女子赛艇八单专项训练强度测试分析与运用

通过监控女子赛艇八单赛前40日训练,对安静状态和不同桨频训练下血乳酸分析,寻找变化规律,找出不同桨频的适宜训练强度,为不同训练负荷提供理论依据。     

Correlation between maximum in-shoe plantar pressures and clubhead speed in amateur golfers

Disagreements exist in the literature regarding the manner in which weight should be dynamically shared during the golf swing, both within-feet and between the back- and target-foot, to generate maximal clubhead speed. The purpose of this study was to determine whether preferential foot-loading locations underlie weight sharing by examining the correlation between clubhead speed and maximum plantar pressure (PP) distributions. Thirty-two amateur golfers with handicap indexes ranging from 2.7 to 25 performed 10 driver swings on artificial turf following a warm-up. PP distributions were recorded at 100 Hz, and clubhead speed was recorded using a ball-tracking Doppler radar system. Maximum PPs were extracted from a 2-s window approximately centred on ball contact and were regressed against clubhead speed. Significance was assessed over the entire foot surface using statistical parametric mapping (SPM), a spatially continuous technique. SPM revealed, at relatively high anatomical resolution, significant positive correlations between clubhead speed and PPs in the lateral target-foot (P < 0.05). This suggests that not only weight transfer but also weight-transfer location may be an important determinant of clubhead speed in amateur golfers.     

渗透压仪研制及其在机电一体化教学中的作用

渗透压仪在医药研究、食品安全等领域应用十分广泛。针对传统的渗透压检测方法存在测量精度低、可靠性差、结构复杂、系统成本高等缺点,提出了一种基于拉乌尔冰点理论的新型渗透压测试仪器,重点介绍了该仪器的原理、实现方案及功能特点,阐述了仪器自主研制对机电一体化教学的作用及意义。     

顺铂联合热处理对术中回收血红细胞及其中混杂的肝肿瘤细胞株(HepG2)的影响（英文）

目的:研究离体热处理和顺铂联合热处理对术中回收血红细胞功能的影响及其中混杂的肝肿瘤细胞株(HepG2)的杀灭作用。创新点:(1)采用多种评价指标研究了不同时间离体热处理对术中回收血中混杂的HepG2的杀灭作用及对红细胞的影响,并确定了对红细胞安全且能有效杀灭HepG2的离体热处理时间。(2)从多个角度评价了离体顺铂联合热处理对术中回收血红细胞的影响及对其中混杂的HepG2的杀灭作用,确定了该方案中对红细胞安全且能有效去除HepG2的顺铂浓度。方法:采用3-(4,5-二甲基噻唑-2)-2,5-二苯基四氮唑溴盐(MTT)、5-乙炔基-2’脱氧尿嘧啶核苷(EdU)和平板克隆形成评估HepG2的细胞存活率、DNA复制率和克隆形成能力(图1和3);从红细胞渗透脆性、携氧能力(2,3-二磷酸甘油酸(2,3-DPG)、半饱和氧分压(P50))、能量代谢(Na+-K+-ATPase、pH)、膜完整性(游离血红蛋白(Hb)、血清K+和Na+浓度、细胞膜磷脂酰丝氨酸外翻比例)等角度评估红细胞功能(图2和4;表3)。结论:肝肿瘤术中回收血经离体顺铂联合热处理(42°C,50μg/ml)60min后,能有效清除其中混杂的HepG2,但对红细胞无显著影响,值得体内进一步研究顺铂热处理有效应用于肿瘤手术自体血液回输的安全方案。     

锦屏一级水电站坝基f2断层水泥灌浆施工技术

断层及层间挤压错动带采用高压水冲洗置换灌浆的方式进行处理,能够冲洗出一部分软弱介质,冲洗后的空腔再采用水泥浆液进行置换固结灌浆,相当于在断层上施加了水泥柱桩,提高了断层基础的承载力。本文介绍锦屏一级水电站大坝左岸f2断层高压冲洗水泥置换灌浆加固施工技术的成功运用。     

FluidSIM在高职液压传动课程改革中的应用

介绍了德国Festo公司液压传动教学仿真软件FluidSIM的功能。结合实例说明了FluidSIM软件在液压传动课程改革中的应用,营造了"教、学、做"一体化的教学环境,有利于激发学生的学习兴趣,提高学习效果。     

SYSMEX UF-1000i尿有形成分分析仪对尿路感染的诊断价值

采用Sysmex UF-1000i尿有形成分分析仪和AX-4280尿干化学分析仪测定尿标本,同时进行尿细菌定量培养。把测定值与尿培养金标准比对,干化学法白细胞酯酶和UF-1000i细菌计数结果的一致性适中,与亚硝酸盐结果的一致性较低,与尿白细胞计数结果的一致性极高。细菌定量培养的特异性、灵敏度及一致性高于UF-1000i白细胞计数。UF-1000i在临床快速诊断尿路感染时应用价值较高。     

补阳还五汤治疗中风后遗症的研究进展

补阳还五汤为清代名医王清任所创,始见于《医林改错》,由黄芪、当归、川芎、地龙、赤芍、桃仁、红花组成,具有补气活血通络的功效。临床的治疗研究与实验证明,本方对于治疗中风后遗症效果良好,尤其以气虚血瘀型效果明显。现代研究表明,补阳还五汤具有抗血栓、抗氧化、抗动脉粥样硬化、和促进神经系统修复等多种作用。文章综述了近年来补阳还五汤对中风后遗症的临床治疗实验及相关研究进展。