不同药物对一次力竭游泳小鼠海马自由基代谢的影响

目的:以人参为阳性对照,研究北冬虫夏草和辽东木对一次力竭小鼠海马自由基代谢的影响及其机制。方法:雄性昆明种小鼠,随机分为5组:安静组,力竭运动组,虫草加力竭运动组,木加力竭运动组,人参加力竭运动组,运动模式为一次无负重力竭游泳,之前6周给相应组大鼠服用北冬虫夏草发酵液、辽东木提取物和人参茎叶提取物,最后一次运动后即刻取样测定各组小鼠海马MDA、T-AOC及游泳至力竭的时间。结果:一次力竭游泳后,运动组小鼠海马MDA含量显著增高(P<0.01),T-AOC显著下降(P<0.01);各服药组小鼠海马MDA含量明显低于运动组(P<0.05),T-AOC显著高于运动组(P<0.01);各服药组小鼠游泳至力竭的时间明显延长(P<0.05)。结论:小鼠在进行一次力竭运动后,海马内脂质过氧化水平升高,总抗氧化能力下降;服用北冬虫夏草、辽东木及人参能够降低力竭游泳小鼠海马脂质过氧化水平,提高总抗氧化能力,并延缓疲劳的发生。     

对推铅球最后用力技术的再认识

从投掷技术原理来讲, 器械飞行的远度主要取决于出手初速度, 而最后用力阶段是其获得速度的主要来源。为适应现代推铅球技术的发展, 文章扼要地提出最后用力开始的时机, 分析影响最后用力效果的因素, 并针对性地提出改进最后用力效果的途径, 对铅球最后用力阶段在技术和理论方面做点滴补充。     

几种增进机能的非兴奋剂使用药物和疗法

提出了关于在体育运动中非兴奋剂使用药物和疗法的观点，介绍了人乳、人血丙种球蛋白、氨基酸，脂肪乳制剂，人参、干姜、当归，回龙汤，能量合剂，暗示疗法，生物全息诊疗法，人工月经周期疗法等增进机能的辅助性非兴奋剂使用药物和疗法。     

中学作文教学原则初探

造成中学作文教学质量和效率低下的根本原因是教学过程和教学方法的非科学化.科学化的作文教学必须从"学习和掌握语言"的本质和规律出发,遵循作文教学的原则,即语文基本能力发展与思维品质培养相结合的原则、先放后收的原则、教师下水的原则.     

竞技场上的“药物战”

简述体育竞赛中使用兴奋剂的几个历史阶段,并通过大量事例来阐述"滥用兴奋剂是国际体育竞赛中最大的公害".     

我国体育新闻发言人制度发展现状分析

运用文献资料、逻辑法,在综合阐述我国新闻发言人制度的基础上,较全面地分析了我国体育新闻发言人制度发展现状:对新闻发言人制度的定位认识不清",官员意识"较浓;没有形成相对固定的新闻发布制度;体育新闻发布的专业人才匮乏;制度不完善,缺乏相应法律的制约和监督。提出了完善我国体育新闻发言人制度的几点建议。     

我国兽药产业发展现状分析与建议

我国兽药产业呈现企业总量规模较庞大,结构差异性与区域性明显,不同类别企业产值、产能利用水平差异大,出厂产品的抽检合格率水平较高且较为稳定等特点。但就产业整体来看,我国兽药产业生产环节存在产业集中水平低,规模不经济;低水平重复建设,利润低;产业结构不合理;产业组织内部管理不规范等问题。因此,亟待政府合理规划布局,加速结构调整;政府与企业联合配置资源,保障产业运行效率;企业内部也需以人为本,重视人才与管理,提升企业自身实力。     

中国古代知识分子孤独心态的表达与消解

古代中国知识分子被称为士人阶层,这个阶层由于对知识的占有和自身的使命感、责任感使然,常常表现出独特的孤独心态,而对这种心态的表达和消解,士人阶层也有其独特的方式和手段,这个过程同时也具有强烈的中国特色。     

Delineation of the Structural Elements of Oriental Liver Fluke PLA2 Isoforms for Potent Drug Designing

Clonorchis sinensis or the Chinese liver fluke is one of the most prevalent parasites affecting a major population in the oriental countries. The parasite lacks lipid generating mechanisms but is exposed to fatty acid rich bile in the liver. A secretory phospholipase A₂, an enzyme that breaks down complex lipids, is important for the growth of the parasite. The enzyme is also implicated in the pathogenesis leading up to the hepatic fibrosis and its complications including cancer. The five isoforms of this particular enzyme from the parasite therefore qualify as potential drug targets. In this study, a detailed structural and ligand binding analysis of the isoforms has been done by modeling. The overall three dimensional structures of the isoforms are well conserved with three helices and a β-wing stabilized by four disulfide bonds. There are characteristic differences at the calcium binding loop, hydrophobic channel and the C-terminal domain that can potentially be exploited for drug binding. But the most significant feature pertains to the catalytic site where the isoforms exhibit three variations of either a histidine-aspartate-tyrosine or histidine-glutamate-tyrosine or histidine-aspartate-phenylalanine. Molecular docking studies show that isoform specific residues and their conformations in the substrate binding hydrophobic channel make unique interactions with certain inhibitor molecules resulting in a perfect tight fit. The proposed ligand molecules have a predicted affinity in micro-molar to nano-molar range. Interestingly, few of the ligand binding interaction patterns is in accordance to the phylogenetic studies to thereby establish the usefulness of evolutionary mechanisms in aiding ligand design. The molecular diversity of the parasitic PLA₂ described in this study provides a platform for personalized medicine in the therapeutics of clonorchiasis.

Electronic supplementary material

The online version of this article (doi:10.1007/s12291-013-0377-1) contains supplementary material, which is available to authorized users.     

False negativity to carbohydrate-deficient transferrin and drugs: a clinical case

Introduction:

In this work we report on the possible effect of the medical therapy on CDT concentration in a chronic alcohol abuser, with known medical history (July 2007 – April 2012) and alcohol abuse confirmed by relatives.

Case history:

At the end of 2007, patient displayed the following laboratory results: AST 137 U/L, ALT 120 U/L, GGT 434 U/L, MCV 101 fL and CDT 3.3%. On December 2007, after double coronary artery bypass surgery, he began a pharmacological treatment with amlodipine, perindopril, atorvastatin, isosorbide mononitrate, carvedilol, ticlopidine and pantoprazole. In the next months, until may 2011, the patient resumed alcohol abuse, as confirmed by relatives; however, CDT values were repeatedly found negative (0.8% and 1.1%) despite elevated transaminases and GGT, concurrent elevated ethyl glucuronide concentration (> 50 mg/L) and blood alcohol concentration (> 1 g/L). Alcohol consumption still continued despite increasing disulfiram doses ordered by an Alcohol Rehab Center. On May 2011, the patient was transferred to a private medical center where he currently lives.

Conclusions:

This study suggests the possibility that a medical therapy including different drugs may hamper the identification of chronic alcohol abusers by CDT.