Proteomic analysis of primary colon cancer-associated fibroblasts using the SELDI-ProteinChip platform

Objective: Cancer-associated fibroblasts (CAFs) are one of the hallmarks of the cancer microenvironment. Recent evidence has indicated that CAFs are more competent in enhancing cancer cell growth and migration than normal fibroblasts. However, the unique protein expression of CAFs has not been fully elucidated. This study aims to investigate the characterizations of colon CAFs by comparing the differential protein expression between CAFs and normal fibroblasts. Methods: Primary fibroblasts were isolated from surgical specimen of human colon cancer and matched normal colonic tissue. Purity of the cell population was verified through immunostain analysis. Total cell lysates and conditioned media from each group of cells were extracted, and protein expression analysis was conducted using the surface-enhanced laser desorption/ionization time-of-flight mass spectrometry (SELDI-TOF-MS) ProteinChip platform. Results: Most primary cells showed typical fibroblast-like features after two weeks. Increased proportion of α-smooth muscle actin-positive myofibroblasts was detected within the CAFs in four of the six pairs of primary cells. Fibroblast activation protein was weakly expressed in most cells without differences. Using SELDI-TOF-MS ProteinChip platform, four protein peaks mass over charge ratio (m/z) 1142, 3011, 4035, and 4945 were detected in the total cell lysates, and two protein peaks m/z 1368 and 1389 were detected in the conditioned media. The potential candidate proteins found in the Swiss-Prot database include morphogenetic neuropeptides, FMRFamide-related peptides, insulin-like growth factor II, thymosin β-4-like protein 3, and tight junction-associated protein 1. Conclusions: Using the SELDI-ProteinChip platform, differential protein expressions were identified in colon CAFs compared with normal colonic stromal fibroblasts. The complex proteomic alternations in colon CAFs may play important roles related to the colon cancer microenvironment.     

激光频率分裂与模竞争实验系统构建

与工厂合作,为研究生专业课"现代光学实验"开发出独创的半外腔型激光频率分裂与模竞争教学实验系统,在同一谐振腔中得到频差较大而偏振态正交的2组激光纵模输出,借助扫描干涉仪和数字示波器可观测和记录2组纵模的频率分裂特性和模竞争现象。研究生通过该实验可深刻理解激光器工作原理和纵模形成机制,并研讨利用激光频率分裂特性进行几何量和物理量的测量方法。     

激光通信综合实验演示仪设计

为了演示激光通信技术的基本原理,介绍了一种激光通信综合实验演示仪的设计原理和工作方法.设计出双模式激光发射、光电转换、功率放大和带通滤波相结合的电路,接收光电探测器采集的激光信号,并还原信号实现解调.该实验仪性能稳定、操作方便,并实现了激光调制与通信演示实验的结合,在实际应用中取得了良好的教学效果.     

基于NPSA算法的ZnO镀膜光纤传感器反射系数研究

根据ZnO镀膜光纤传感器的几何结构和等效网络模型,建立了目标函数压电层反射系数模型。提出了一种交叉算子的小生境粒子群算法,优化后的压电层反射系数收敛性较高,入射波的接收效率大大提高。得出了一系列器件参数数据,为ZnO镀膜光纤声光相位传感器的制作提供了理论参考。     

稳功率氦氖激光器电源的研制

一种新型氦氖激光器电源以直流12V输入,并采用开关电源逆变、倍压整流等技术;摒弃了传统的大电阻恒流的方式,采用了负反馈电路控制电流,减少了功耗,且稳流精度高于2％;还增加了稳功率电路,稳功率精度可达3％以上。     

三维彩色扫描系统及算法

提出了一种大尺寸 3D物体几何形状的扫描测量系统 ,该系统由一个线结构光源和一个彩色CCD摄像机组成 .CCD摄像机两次摄取被扫描物体的图像 ,首先从激光平面中获取被扫描物体的三维信息 ,然后将 3D物体的彩色信息和三维信息自动进行叠合 ,以获得 3D物体的真实模型 .     

激光的振幅和位相调制诱导分子的局域模振动

在两激光场与三能级分子相互作用系统中，调制光场的振幅和位相，诱导分子的局域模振动，并讨论了分子的动力学规律     

对量子噪声实虚部互关联情况下饱和激光模型光强涨落的分析

针对实、虚部关联的量子噪声及泵噪声驱动的饱和激光模型,基于近似福克—普郎克方程,推导激光强度定态概率分布函数,利用投影算子法,分析了由于量子噪声实虚部关联度λq导致锁相时的光强度关联函数C(τ)和集中驰豫时间Tc。通过计算机数据计算及作图,得到了定态情况下光强概率分布函数图形,讨论了量子噪声实、虚部的互关联对光强关联函数和集中驰豫时间的影响。结果表明:量子噪声实、虚部互关联会导致激光强度涨落增大。     

Er^3＋-Yb^3＋ Co-Doped Fiber Ring and Line Laser

Er^3 -Yb^3 co-doped fiber of 2 m long is used as the laser gain medium. Two fiber lasers with different structures have been set up, one is the line cavity fiber laser with the dielectric mirror being replaced by an all-fiber reflecting mirror,the other is the ring cavity all-fiber laser. Both set-ups have achieved lasing operation at the wavelength of 1.53 μm. Pumped by the 1 064 nm light from all-solid-state Nd : YAG laser, the two fiber lasers at 1 530 nm are operational. Their output powers are 7.8 mW and 2 mW with 130 mW and 160 mW pump powers.     

便携式半导体激光治疗仪的设计

采用10毫瓦半导体激光管,以AT89C2051单片机、TPS76333稳压块、CA358运算放大器等集成电路为核心,设计经济实用的激光治疗仪,给出了硬件电路和编程方法。同时,论证了治疗仪设计的合理性和经济实用性。