大强度训练对自行车运动员淋巴细胞亚群和NK细胞活性的影响

本文观察了大强度训练对自行车运动员外周血T淋巴细胞亚群和NK细胞活性的影响。实验结果发现,经过四周大强度训练,CD8 细胞比例显著上升(P<0.05),CD4 /CD8 比值显著下降(P<0.05),NK细胞活性显著下降(P<0.05)。提示大强度训练造成机体免疫功能下降与抑制性T淋巴细胞过度激活,继发造成免疫平衡失常和NK细胞活性下降有关。     

超负荷训练对大鼠心脏左室心肌纤维及微血管的影响

以 SD大鼠为实验对象 ,并对其进行了超负荷实验研究。结果表明 ,超负荷训练对大鼠的心血管系统影响较大。实验组与对照组的三项测试指标对比均有显著性差异 (P <0 .0 5 )。分析造成其结果的主要原因是持续的超负荷训练使鼠体各器官的机能得不到恢复和补偿 ,以至导致鼠心血管系统向病理性方向发展。这一结果证明 ,把握好超负荷训练的持续时间、强度和超量恢复对心血管系统至关重要。     

木兰拳练习对女性高血压病患者红细胞变形性的影响——与红细胞膜脂质成分关系的初步研究

采用实验测试的方法，研究长期规律木兰拳练习对绝经期女性高血压病患者红细胞变形性的影响，以及红细胞变形性与红细胞膜脂质成分的可能关系。结果发现，在绝经期进行长期规律木兰拳练习，可以显著改善女性高血压病患者红细胞膜脂质成分，这种红细胞膜脂质成分的改善，包括膜胆固醇(CHO)与膜C／PL比值．与红细胞变形能力的改善有密切关系。     

心房肌细胞的特殊颗粒形态和机能的研究

简要综述了心房肌细胞的特殊颗粒形态学特征、分布与其它细胞器的关系，特殊颗粒的组织化学、心房肽类物质的提纯和作用以及运动对心钠素影响等问题，从而对心脏在循环系统中的作用带来某些新的认识，对于心脏功能传统概念将会带来某些修正和补充。     

提高细胞生物学教学效果的途径

细胞生物学是生命科学的重要基础学科,是生物科学、生物工程专业必修课程之一．在更新教学内容、优化知识结构、改革教学方法和组织课堂讨论等方面进行了探索和改进,提高了教师的教学质量和学生的学习积极性．     

山寨机的成因、困境和对策分析——以深圳为例

以深圳＂山寨机＂为例,分析了山寨机出现的经济原因、政策原因、技术原因、价格原因、产业链原因、产品推出速度原因、外观设计原因、功能原因、利益链重构原因,指出了＂山寨化＂的深层次根源在于中国产业的＂三元化＂,分析了＂山寨机＂面临的市场困境、价格困境、技术困境、信任困境、品牌困境、生存困境,并在此基础上提出山寨厂商、行业协会、政府部门应各负其责、营造更好产业成长环境的未来出路。     

医学细胞生物学考试方法的改革与实践

为加强和优化医学细胞生物学教学,促进教育从应试型向素质型转变,对学生成绩的考核进行了改革,采用一种新的综合考核方式。结果表明,82.2%的同学赞同这种考试形式的改革,认为起到了加强素质教育的作用。此外,还对进一步完善这种考试形式的改革进行了讨论,并提出了建议。     

Metachronous contralateral testicular and bilateral adrenal metastasis of chromophobe renal cell carcinoma: a case report and review of the literature

Chromophobe renal cell carcinoma(ChRCC) metastatic to the testis has not,to the best of our knowledge,been reported in the literature.Nor have there been reports of delayed bilateral adrenal metastasis of ChRCC.Here we report a case of metachronous contralateral testicular and bilateral adrenal metastasis of ChRCC in a 70-year-old man who underwent right radical nephrectomy for RCC six years ago.He was admitted to the hospital because of left intrascrotal enlargement of two-month duration.Ultrasonography re...     

Heat shock protein 90 protects rat mesenchymal stem cells against hypoxia and serum deprivation-induced apoptosis via the PI3K/Akt and ERK1/2 pathways

Mesenchymal stem cell(MSC)transplantation has shown a therapeutic potential to repair the ischemic and infracted myocardium,but the effects are limited by the apoptosis and loss of donor cells in host cardiac microenvironment.The aim of this study is to explore the cytoprotection of heat shock protein 90(Hsp90)against hypoxia and serum deprivation-induced apoptosis and the possible mechanisms in rat MSCs.Cell viability was determined by3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide(MTT)assay.Apoptosis was assessed by Hoechst 33258nuclear staining and flow cytometric analysis with annexin V/PI staining.The gene expression of Toll-like receptor-4(TLR-4)and V-erb-b2 erythroblastic leukemia viral oncogene homolog 2(ErbB2)was detected by real-time polymerase chain reaction(PCR).The protein levels of cleaved caspase-3,Bcl-2,Bcl-xL,Bax,total-ERK,phospho-ERK,totaI-Akt,phospho-Akt,and Hsp90 were detected by Western blot.The production of nitric oxide was measured by spectrophotometric assay.Hsp90 improves MSC viability and protects MSCs against apoptosis induced by serum deprivation and hypoxia.The protective role of Hsp90 not only elevates Bcl-2/Bax and Bcl-xL/Bax expression and attenuates cleaved caspase-3 expression via down-regulating membrane TLR-4 and ErbB2 receptors and then activating their downstream PI3K/Akt and ERK1/2 pathways,but also enhances the paracrine effect of MSCs.These findings demonstrated a novel and effective treatment strategy against MSC apoptosis in cell transplantation.     

Effect of high glucose levels on the calcification of vascular smooth muscle cells by inducing osteoblastic differentiation and intracellular calcium deposition via BMP-2/Cbfα-1 pathway

In this paper,we investigate the effect and the possible mechanism of high glucose levels on the calcification of human aortic smooth muscle cells (HASMCs).HASMCs were divided into four groups: normal glucose group (NG),osmolality control group (OC),high glucose group (HG,HASMCs culture medium containing 30 mmol/L glucose),and high glucose plus recombinant human Noggin protein (bone morphogenetic protein-2 (BMP-2) antagonist) group (HN).The mRNA levels and the protein expressions of BMP-2 and core binding factor alpha-1 (Cbfα-1) were measured by real-time quantitative polymerase chain reaction (PCR) and Western blot.After induced by 10 mmol/L β-glycerol phosphoric acid,cells were harvested for assessments of alkaline phosphatase (ALP) activities at Days 1,2,and 3,and intracellular calcium contents at Days 7 and 14,respectively.High glucose levels increased the mRNA levels and the protein expressions of BMP-2 and Cbfα-1 (P0.05).The expression of Cbfα-1 was partially blocked by Noggin protein (P0.05),while BMP-2 was not (P0.05).After being induced by β-glycerol phosphoric acid,high glucose levels increased the ALP activity [(48.63±1.03) vs.(41.42±2.28) U/mg protein,Day 3;P0.05] and the intracellular calcium content [(2.76±0.09) vs.(1.75±0.07) μmol/mg protein,Day 14;P0.05] in a time-dependent manner when compared with the NG group,while the ALP activity could not be blocked by Noggin protein [(48.63±1.03) vs.(47.37±0.97) U/mg protein,Day 3;P0.05].These results show that high glucose levels can evoke the calcification of HASMCs by inducing osteoblastic trans-differentiation and intracellular calcium deposition via the BMP-2/Cbfα-1 pathway,which can be partially blocked by Noggin protein.