Purification and relationship with gastric disease of a 130 kDa (CagA) protein of<Emphasis Type="Italic">Helicobacter pylori</Emphasis>

Objective: The aims of this research were to purify and identify the 130 kDa (CagA) protein ofH. pylori clinical isolate HP97002 and evaluate the relationships between the purified 130 kDa (CagA) protein and gastric diseases. Methods: The procedure for isolating the protein included 6 mol/L guanidine extract, size exclusion chromatography and elusion from gel. Sera of 68 patients with gastric diseases (44 with chronic gastritis, 15 with atrophic gastritis, 7 with peptic ulcer disease, 2 with gastric cancer) were obtained, and the serological response to CagA was studied by Western-blot using the purified protein. Results: The purified protein was 130 kDa and preserved good antigenicity and revealed basic isoelectric point about of 8.1. Among 68 sera, 43 sera could recognize the purified protein associated with chronic gastritis 47.7% (21/44), atrophic gastritis 86.7% (13/15), peptic ulcer disease 100% (7/7), gastric cancer 100% (2/2). Compared with each other, the difference was significant (χ²=13.327,P=0.004), and 130 kDa (CagA) protein was associated with severe gastric diseases (r _s=0.442,P=0.001). Conclusion: The 130 kDa (CagA) protein was associated with severe gastric diseases. Project supported by the China Medical Board (96-628) and Zhejiang Province Hygiene Bureau (2000 A055)     

顶空进样—毛细管柱气相色谱法分析啤酒的香味组分

介绍了采用毛细管柱气相色谱法分析测定啤酒中的微量挥发性香味组分.以高纯N2(99.999%)作为载气,并结合自动顶空进样技术,对啤酒中的12种微量挥发性香味组分进行定性和定量分析.结果表明:变异系数除乙醛与辛酸乙酯外均小于5%,回收率均在90%以上.该法具有较好的重现性和准确度.     

纳米晶铝酸锌的合成及表征

以硬脂酸法合成了纳米晶铝酸锌并用TG—DTA，XRD和TEM对其进行了表征．结果表明，纳米晶粒径小，粒度均匀，且呈尖晶石结构；纳米晶的粒径随烧结温度的变化而变化．     

纵向整体—分段连接:师范生语文教师专业技能训练体系

师范生语文教师专业技能"纵向整体—分段连接"的训练体系,是在"大语文教育"观和"整合连贯型"教师教育培养模式的背景下产生的,是对以往诸如"横向整体—学科渗透"训练体系的补充。其内涵是:注重语文教育教学作为一个整体却又具有小学、初中、高中的阶段特征,将师范生语文教师专业技能训练中的这三个阶段全面贯通、整体融合。培养既能承担小学语文教学,又能承担初中、高中语文教学的高素质语文教学师资。     

直接液相微萃取-高效液相色谱法测定水中速灭威残留

农药的使用对农业有重要的、不可替代的意义,然而很多农药的使用会给环境带来污染,影响人类健康.本文采用直接液相微萃取法（Dir-LPME）与高效液相色谱（HPLC）联用分析测定环境水样中的速灭威.对影响Dir-LPME的各种因素如萃取溶剂类型、萃取溶剂体积、萃取时间等条件进行了优化,并将其应用于江水、自来水和农田水中速灭威的分析检测.速灭威的萃取富集倍数为10,线性范围为0.2-2μg/mL,加标回收率在95%—105%之间,相对标准偏差低于7.4%.     

Relative bioavailability and pharmacokinetic comparison of two different enteric formulations of omeprazole

In order to comply with the requirements for a drug listed in China, the study was developed to compare the pharmacokinetics and relative bioavailability of two different enteric formulations of omeprazole (OPZ) in healthy Chinese subjects. A total of 32 volunteers participated in the study. Plasma concentrations were analyzed by nonstereospecific liquid chromatography/tandem mass spectrometric (LC-MS/MS) method. After administration of a single 40-mg dose of the two OPZ formulations, the comparative bioavailability was assessed by calculating individual AUC_0−t (the area under the concentration-time curve from time zero to the last measurable concentration), AUC_0−∞ (the area under the concentration-time curve extrapolated to infinity), C _max (the maximum observed concentration), and T _peak (the time to C _max) values of OPZ, 5-hydroxyomeprazole (OH-OPZ), and omeprazole sulfone (OPZ-SFN), respectively. The 90% confidence intervals (CIs) of AUC_0−t , AUC_0−∞, and C _max were 85.4%–99.0%/88.8%–98.6%/87.6%–99.4%, 85.5%–99.2%/89.0%–98.6%/88.5%–101.3%, and 72.3%–87.6%/79.6%–91.1%/88.4%–99.1% for OPZ/OH-OPZ/OPZ-SFN, respectively, and T _peak values did not differ significantly. In this study, the test formulation of OPZ in fasting healthy Chinese male volunteers met the Chinese bioequivalance standard to the reference formulation based on AUC, C _max, and T _peak.     

高效液相色谱法测定武夷橙中的有机酸

利用反相高效液相色谱法研究了武夷橙中7种有机酸的分离测定条件。采用C18为分析柱,以2.5%磷酸二氢铵缓冲溶液（PH=2.5）为流动相,流速为1mL/min,紫外检测波长在210nm处可以很好的分离测定有机酸。结果表明峰面积与相应的质量浓度有良好的线性关系,其r大于等于0.9990,平均回收率为98%～110%,该方法具有测定结果准确可靠,重现好等特点。     

混合型寡糖手性固定相的制备

先制备了纤维二糖手性固定相和棉子糖手性固定相两种手性固定相,然后制备了混合型手性固定相,以正己烷∶异丙醇=90∶10为流动相,拆分2种手性药物,没有拆分开.结果表明,混合型寡糖手性固定相拆分效果不好.     

金属-有机骨架材料在色谱分离中的应用

金属-有机骨架(MOFs)材料作为一种新型功能材料由于其特殊的属性吸引了许多研究者的目光.本文讨论了近年来手性与非手性金属-有机骨架材料作为固定相在气相色谱(GC)和高效液相色谱(HPLC)分离中的应用;并对这种新型功能材料在色谱分离中的广阔应用前景作了展望.     

凝胶燃烧法制备镍掺杂氧化锌及其光致发光性能

采用凝胶燃烧法制备了粒径为100 nm~300 nm 的 Zn1-xNixO (x=0,0.01,0.02,0.03,0.04)纳米粉末.使用 SEM、 XRD、荧光分光光度计对产物的结构、形貌及光学性能进行表征.研究结果表明,氧化锌中镍的最大掺杂量为 x=0.03,超出以后会析出氧化镍晶体.随着镍掺杂量的增加,镍掺杂氧化锌的紫外吸收限发生红移,能带宽度逐渐降低,紫外光区的吸收减弱而可见光区的吸收增强;镍掺杂氧化锌发射光谱发射峰强度减弱、宽化,并出现一些新的发射峰