Transgenic barley with overexpressed PTrx increases aluminum resistance in roots during germination

A transgenic barley line (LSY-11-1-1) with overexpressed Phalaris coerulescens thioredoxin gene (PTrx) was employed to measure the growth, protein oxidation, cell viability, and antioxidase activity in barley roots during germination on the presence of 2 mmol/L AlCl₃ on filter paper. The results show that (1) compared with the non-transgenic barley, LSY-11-1-1 had enhanced root growth, although both were seriously inhibited after AlCl₃ treatment; (2) the degree of protein oxidation and loss of cell viability in roots of LSY-11-1-1 were much less than those in roots of non-transgenic barley, as reflected by lower contents of protein carbonyl and Evans blue uptakes in LSY-11-1-1; (3) activities of catalase (CAT), glutathione peroxidase (GPX), ascorbate peroxidase (APX), and glutathione reductase (GR) in LSY-11-1-1 root tips were generally higher than those in non-transgenic barley root tips, although these antioxidase activities gave a rise to different degrees in both LSY-11-1-1 and non-transgenic barley under aluminum stress. These results indicate that overexpressing PTrx could efficiently protect barley roots from oxidative injury by increasing antioxidase activity, thereby quenching ROS caused by AlCl₃ during germination. These properties raise the possibility that transgenic barley with overexpressed PTrx may be used to reduce the aluminum toxicity in acid soils.     

个人化因子：对宏大叙事的潜在拆解——以《青春之歌》为例

《青春之歌》是一部以知识分子思想改造为题材的“红色经典”小说,但主角林道静在长成的道路上,留下了大量“非革命化”的痕迹,尤其是爱情纠葛中的内心傍徨与痛苦,使“革命’’染上了人道主义的凄楚与温情。正是这些因素使作品成为描述“革命”的独特文本,获得了比较持久的艺术生命力。     

基因指纹和芯片技术在生物资源调查和标本整理中的应用

随着PCR 技术的广泛应用,基因指纹和芯片技术得到快速发展,已成为分子生物学研究的重要手段并得到广泛应用。文章首先综述了目前常用的基因指纹和芯片技术及其优缺点。常用的技术有：DNA 限制性片断长度多态性分析（RFLP）、随机扩增多态性DNA 分析（RAPD）、扩增片断长度多态性分析（AFLP）、微卫星DNA 多态性分析（SSR）、单核苷酸多态性分析（SNP）等。然后简要概述这些技术在生物资源调查和标本整理中的应用。主要应用于物种鉴定和分类、个体识别和种群数量调查、资源植物关键信息研究等。最后展望这些技术在     

论我国未决羁押制度的现状与完善

未决羁押在我国已经是一种对待犯罪嫌疑人的常态化处置方式,由于混同了逮捕与羁押,导致未决羁押司法审查的缺失及司法救济的虚无。应当从根本上对未决羁押制度进行完善,实现逮捕与羁押相分离,设置独立的羁押审查程序,让中立的司法机关对羁押的根据进行司法审查,从而将未决羁押纳入司法控制的范畴,赋予被羁押者对羁押合法性提出质疑的权利,利用司法权来保障被羁押者免受非法羁押。     

Gene editing in plants: progress and challenges

The clustered regularly interspaced short palindromic repeat (CRISPR)-associated protein 9 (Cas9) genome editing system is a powerful tool for targeted gene modifications in a wide range of species, including plants. Over the last few years, this system has revolutionized the way scientists perform genetic studies and crop breeding, due to its simplicity, flexibility, consistency and high efficiency. Considerable progress has been made in optimizing CRISPR/Cas9 systems in plants, particularly for targeted gene mutagenesis. However, there are still a number of important challenges ahead, including methods for the efficient delivery of CRISPR and other editing tools to most plants, and more effective strategies for sequence knock-ins and replacements. We provide our viewpoint on the goals, potential concerns and future challenges for the development and application of plant genome editing tools.     

有氧能力关联基因多态与技巧类滑雪运动员跨项选材

目的：研究优秀技巧类滑雪运动员中4个有氧能力关联多态基因ACE、GLUT4、ADRB2和PPARGC1A的分布频率，及基于4个优势等位基因的基因型总分（TGS），探索上述多态基因与TGS作为我国优秀技巧类滑雪运动员跨项选材分子标记的可行性。方法：应用基质辅助激光解吸附电离飞行时间质谱检测技术，对68名中国北方汉族优秀技巧类滑雪运动员与138名中国北方汉族普通大学生ACE基因rs4340位点、GLUT4基因rs5418位点、ADRB2基因rs1042713位点和PPARGC1A基因rs8192678位点进行解析。计算并比较运动员与对照组在上述4个位点的TGS。结果：在上述4个多态位点，运动员与对照组间的基因型和等位基因频率无显著性差异（P>0.05）；两组人在携带的优势等位基因数量、TGS分值范围、平均TGS分值及TGS分值的分布频率上也均无显著性差异（P>0.05）。结论：ACE基因rs4340位点、GLUT4基因rs5418位点、ADRB2基因rs1042713位点、PPARGC1A基因rs8192678位点多态性和基于上述4个位点的TGS均无法作为中国北方汉族技巧类滑雪运动员的有氧能力跨项分子选材标记。     

Study on pig growth hormone gene polymorphisms in western meat-type breeds and Chinese local breeds

INTRODUCTIONGrowthhormone (GH)isapeptidehormonewhichregulatesgrowthandvariousmetabolicac tivities (Sterle,1 995;Yuan ,1 996) .InjectionsofGHintogrowingpigsincreasedgrowthrateoftheanimalsandthepercentageofmuscle ,andwhilefataccretionwasdecreased (Bonneau ,…     

审美理想主义与乡村散文创作

中国的诗文审美文化即乐感文化,是审美理想主义滋生形成的土壤和基础。这种审美理想主义对我国现当代散文特别是“乡村散文”创作的负面影响尤为明显,其影响之所以至今余绪不断,也与“另一部分作家”对“另一种文章”的迷恋和寻找有关。欲振兴“乡村散文”写作,必得要糅人“杂感文”的“否定性”因子,如此,“乡村散文”创作才有生机和潜力。     

钩端螺旋体黄疸出血群赖株mviN基因的克隆

采用高保真PCR从我国钩体黄疸出血群赖株基因组中扩增全长mviN基因片段,对钩体黄疸出血群赖株mviN基因进行克隆.结果显示,所克隆的mviN基因的核苷酸和氨基酸序列与已发表的mviN基因序列(Accesion No.in GenBank: NC004343)同源性分别为99.68%和99.42%.因此可以认为,我们成功克隆了mviN基因,并为该基因的后续研究奠定了基础.