蟋蟀基因组DNA提取方法

对直翅目蟋蟀总科部分种类酒精浸泡标本的基因组DNA进行了提取，样品经检测，谱带基本呈线形，无拖尾；OD(260／280nm)值86．11％在1．6—1．8之间。     

利用随机扩增多态性DNA技术对金丝小枣9个品种的资源分析

金丝小枣是我国优良的枣类品种之一.对金丝小枣基因组DNA提取方法进行了研究,探索出了一种适合金丝小枣基因组DNA提取的方法,并利用提取的DNA对金丝小枣系列9个品种进行了RAPD技术分析鉴定,找到了特殊谱带,构建了金丝小枣系列的分子标记检索表,为准确鉴定金丝小枣种质资源提供了分子生物学依据.     

Exploring criteria for successful query expansion in the genomic domain

Query Expansion is commonly used in Information Retrieval to overcome vocabulary mismatch issues, such as synonymy between the original query terms and a relevant document. In general, query expansion experiments exhibit mixed results. Overall TREC Genomics Track results are also mixed; however, results from the top performing systems provide strong evidence supporting the need for expansion. In this paper, we examine the conditions necessary for optimal query expansion performance with respect to two system design issues: IR framework and knowledge source used for expansion. We present a query expansion framework that improves Okapi baseline passage MAP performance by 185%. Using this framework, we compare and contrast the effectiveness of a variety of biomedical knowledge sources used by TREC 2006 Genomics Track participants for expansion. Based on the outcome of these experiments, we discuss the success factors required for effective query expansion with respect to various sources of term expansion, such as corpus-based cooccurrence statistics, pseudo-relevance feedback methods, and domain-specific and domain-independent ontologies and databases. Our results show that choice of document ranking algorithm is the most important factor affecting retrieval performance on this dataset. In addition, when an appropriate ranking algorithm is used, we find that query expansion with domain-specific knowledge sources provides an equally substantive gain in performance over a baseline system.

IGF2基因的遗传学特征与生物学意义

IGF2基因是最早发现的内源性印迹基因,其印迹异常参与了许多遗传病尤其肿瘤的发生,在胎儿发育、肿瘤细胞增殖等方面具有重要的调控作用。本文主要阐述了,IGF2基因的遗传学特征、表达机制、生物学意义及其与疾病发生的关系等。     

玉米基因组DNA的提取

玉米（ｚｍａｙｓ）鲜叶不经冻干处理直接用ＣＴＡＢ法提取ＤＮＡ，其分子量和纯度均较高，可用于限制切、基因文库构建及基因组分析等．     

Cloud Computing for Voxel-Wise SEM Analysis of MRI Data

As data collection costs fall and vast quantities of data are collected, data analysis time can become a bottleneck. For massively parallel analyses, cloud computing offers the short-term rental of ample processing power. Recent software innovations have reduced the effort needed to take advantage of cloud computing. To demonstrate, we replicate a voxel-wise examination of the genetic contributions to cortical development by age using evidence from 1748 MRI scans. Specifically, we employ off-the-shelf Kubernetes software that permits us to re-run our analyses using almost the same computer code as was published in the original article. Large, well funded institutions may continue to maintain their own computing clusters. However, the modest cost of renting and ease of utilizing cloud computing services makes unprecedented compute power available to all researchers, whether or not affiliated with a research institution. We expect this to spur innovation in the sophisticated modeling of large datasets.     

Three Is Better Than Two,for Suffered Bacteria Living under Stress

<正>In response to environmental signals,the expression levels of responsive genes of organisms have to change accordingly for long-term survival.This process is far more complicated than a simple"on-off"switch,but has a subtle pattern that is subject to tight regulation.Among the known kinetic patterns,expression     

心肺健康特征指标有氧耐力训练效果的遗传预测标记研究进展

运动训练后个体的机能水平受多种因素影响,其中环境和遗传因素在很大程度上起决定作用。某一群体可能因携带特定基因型对运动训练 具有更高的敏感性,从而表现出更好的训练效果。训练效果的遗传学研究已开展40余年,在有氧耐力训练效果的个体差异领域,研究最系统,成果 也最丰富,但有关心肺健康特征指标有氧耐力训练效果的遗传预测标记研究方兴未艾。为此,综述了迄今为止该领域的候选基因法及全基因组研 究进展,旨在为相关研究的开展提供参考和研究方向。综述后发现,40余个基因的愈百位点/单体型与我国北方汉族人的有氧耐力训练敏感性相 关,血管紧张素转化酶基因等18个候选基因的作用则有待商榷。在全基因组研究中,训练后在50 W功率输出水平下每搏量（SV50）和亚极量运动 心率（HR50）分别与驱动蛋白家族成员5B基因编码区内的rs211302和位于cAMP反应元件连接蛋白1基因座5’区的rs2253206及rs2360969相关联; 通过将转录物组学与基因组学技术结合,获得了11个可共同影响23%VO2max训练效果变化量的SNPs;而在多个HERITAGE项目研究中,则分别发 现了与45%的VO2max训练效果个体差异显著相关的16个SNPs,100%影响HR50训练效果个体异质性的9个SNPs,以及20%影响20周标准耐力训 练后60% VO2max强度运动下摄氧量（△VO260）的13个SNPs及单体型。     

我国橡胶树育种的技术瓶颈与创新发展建议

橡胶树优良品种是实现天然橡胶稳产保供的核心要素。我国植胶区地处热带北缘,属于非传统植胶区,气候等条件劣于东南亚传统植胶区,致使已种植的橡胶树频繁遭受低温寒害和病害,因而生产上急需高产抗逆品种。导致橡胶树高产抗逆品种贫乏的主要原因包括：橡胶树基因组高度杂合、童期长和异花授粉等特性,使得高产性状和抗逆性状不易聚合;橡胶树育种的选择方法效率低,不仅杂交分离群体小,而且依赖长期大田鉴定。建议：应用全基因组选择育种理念,尽快构建橡胶树高世代种子园,以实现多性状聚合育种;设立专门的技术支撑岗位持续扩大育种群体规模,打造橡胶树高效育种技术体系。     

药用植物虎杖毛状根DNA提取方法研究

采用高盐低pH法、改良SDS法、CTAB法、改良CTAB法提取发根农杆菌ATCC11325感染蓼科多年生药用植物虎杖（Polygonum cuspidatum Sieb．et Zucc．）叶片产生毛状根的基因组DNA,并进行了电泳分析、含量检测及特异PCR扩增．结果表明：改良CTAB法解决了虎杖富合多酚、多糖等类物质难以提取高质量DNA的问题,提取的DNA质量较好,纯度较高,能满足进一步的实验要求．