Protective influence of vitamin E on the antioxidant defence system in the whole blood and liver of normal and alloxan-induced diabetic rats

The effect of oral administration of vitamin E for twenty-eight consecutive days on blood glucose, reduced glutathione levels, antioxidant enzymes (superoxide dismutase and catalase activities), and levels of malondialdehyde (as an index of free radical-mediated lipid peroxidation) was observed in the whole blood and liver of normal and alloxan-induced diabetic rats. It was found that oral administration of vitamin E significantly (p<0.05) lowered the blood glucose level and increased the body weight of the diabetic rats. The activities of superoxide dismutase and levels of reduced glutathione increased significantly (p<0.05) while the level of lipid peroxidation decreased.     

运动训练和谷胱甘肽

采用资料法分析了谷胱甘肽的生理功能以及运动训练对谷胱甘肽的影响。表明,保持体内谷胱甘肽的浓度,对防止运动疲劳的自由基损伤、提高运动能力和消除疲劳具有重要作用     

金樱子果肉Cu·Zn—SOD纯化及其活性测定

通过（ＮＨ４）２ＳＯ４分级沉淀和ＳｅｐｈａｄｅｘＧ—１００分离提纯得到金樱子Ｃｕ·Ｚｎ—ＳＯＤ，经聚丙烯酞胺凝胶电泳发现有５—６条同工酶谱带，最明显的３条迁移率分别为０．４３，０．４６和０．５５，ＳＤＳ—ＰＡＧＥ测得亚基分子量约为１３ＫＤ，其活性被２ｍｍｏｌ／ＬＫＣＮ或Ｈ２Ｏ２抑制，不受氯仿—乙醇影响，在ｐＨ７．６磷酸缓冲液中活性最大，对热相对稳定。ＳＯＤ活性与ＶＣ、ＧＳＨ含量有明显的相关性，相关系数为０．９３４２和０．９１０６，果实由金黄转褐黄阶段有—ＳＯＤ活性高峰。认为以Ｖｃ为指标可以判断ＳＯＤ活性水平。     

GST-π在乳腺癌中的表达及意义

目的:探讨GST-π在乳腺癌中的表达及意义.方法:采用免疫组化的方法对GST-π在47例乳腺癌中的表达及临床意义进行研究.结果:GST-π总的阳性率为61.70%(29/47),在浸润性导管癌、单纯癌、髓样癌中的阳性率分别为62.06%、83.33%、50.00%.GST-π在乳腺癌中的表达与年龄、病理学类型及淋巴结转移无明显相关性.结论:GST-π在乳腺癌中的表达率较高,且与耐药有关,应重视对乳腺癌中GST-π表达的检测,以便优化化疗方案.     

MET对油菜幼苗活性氧代谢的影响

试验采用质量比值分别为100×10(-6),150×10~(-6),200×10~(-6)的MET喷施二叶一心期油菜幼苗,于第7,14,21(或23)和45d随机取功能叶,以比色法测定POD活性,滴定法测CAT活性及GSH含量,抗坏血酸过氧化物酶活性采用紫外吸收法测定,结果表明,喷施多效唑的油菜幼苗叶中POD和抗坏血酸过氧化物酶活性增高,CAT活性降低,GSA含量增加,从而说明了MET提高作物的抗逆性,可能是由于提高某些保护酶活性及增加非酶类自由基净化剂含量所致。     

棒叶落地生根叶片中抗坏血酸-谷胱甘肽循环对干旱与复水的响应

棒叶落地生根植株干旱20d与70d,并对干旱70d的植株进行复水处理,测定叶片中抗坏血酸过氧化物酶（APX）、谷胱甘肽还原酶（GR）与脱氢抗坏血酸还原酶（DHAR）的活性,以及抗坏血酸（AsA）与谷胱甘肽（GSH）含量的变化。结果表明,随着干旱进行,APX与DHAR活性均显著增加,GR活性先增加后降低;AsA、脱氢抗坏血酸（DHA）含量与AsA／DHA比值均显著增加,GSH含量增加但不显著,氧化型谷胱甘肽（GSSG）含量显著增加,GSH／GSSG比值增加但不显著。复水后叶片中APX、DHAR活性显著低于干旱70d时,GR活性高于干旱70d时;AsA、DHA含量与AsA／DHA比值显著低于干旱70d时,GSH、GSSG含量与GSH／GSSG比值低于干旱70d时,但差异不显著。可见,在棒叶落地生根遭遇干旱时,AsA—GSH循环系统清除活性氧的能力提高。     

昆虫谷胱甘肽硫转移酶农药解毒与内源代谢研究进展

谷胱甘肽S-转移酶（Glutathione S-transferase,GST）是生物体内重要的解毒酶系之一,广泛存在于动物、植物和微生物中。目前有关昆虫GST的研究多集中在其对农药的抗性机制及代谢解毒方面,但深入的分子机制尚有待深入研究。本文结合国内外昆虫GST的最新研究成果以及课题组多年来对飞蝗GST的研究成果,着重从分子水平介绍昆虫GST基因对农药代谢解毒机制及其参与内源物质代谢机理的研究进展。对昆虫GST分子机制的研究,将有助于深化对昆虫GST的抗药性机制和生理功能的认识,发现新的分子靶标,为新型杀虫剂的研发和农林害虫的防治提供新思路。     

Protection of salvianolic acid B for human endothelial cells against hydrogen peroxide-induced oxidative damage

Salvianolic acid B(Sal B) is an active component of traditional Chinese medicine Salvia miltiorrhiza and is used to treat vascular diseases. To better understand its mechanism, the antioxidant capacities of Sal B was evaluated with human endothelial cells under oxidative stress. Human endothelial cells were pretreated with Sal B for 12 h followed by hydrogen peroxide for another 12 h. Production of reactive oxygen species (ROS), activities of superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPX), and concentration of glu-tathione were measured: Protective effect of Sal B on the endothelial cells from hydrogen peroxide-induced damage ' was observed, and ROS production in the cells was found significantly inhibited. Sal B remarkably enhanced the activities of antioxidant enzymes SOD, CAT and GPX. Furthermore, Sal B up-regulated the intracellular glutathione concentration. The results indicate that Sal B protected endothelial cells from oxidative stress by improving the redox status of the cells through enhancing the antioxidant enzyme activities and increasing the reductive glutathione concentration after the oxidative challenge.     

老年人血小板谷胱甘肽过氧化物酶的纯化和分子特性研究

报道老年人血小板谷胱甘肽过氧化物酶由ＳｅｐｈａｒｏｓｅＱ柱层析、ＭｏｎｏＱＨＲ５／５ＦＰＬＣ和亲和层析后，经ＳＤＳ／ＰＡＧＥ鉴定为一条区带，其分子量为２３ｋＤａ．等电聚焦测定ＰＩ为５．１．用Ｓｕｐｅｒ－ｏｓｅＨＲ１０／３０柱经ＦＰＬＣ测定的分子量为９２ｋＤａ，这表明在自然状态下血小板谷胱甘肽过氧化物酶由四个相同的亚单位组成。这些结果与人红细胞谷胱甘肽过氧化物酶的特性相一致．     

外源谷胱甘肽对水稻种子萌发过程中铜毒害的缓解效应

采用种子萌发试验,研究了10μmol.L-1CuCl2胁迫下,外源谷胱甘肽对水稻种子萌发过程中铜毒害的缓解效应.结果表明,100mg.L-1的外源谷胱甘肽能够显著提高水稻种子的发芽率、发芽指数及活力指数.10μmol.L-1CuC l2对幼根的生长及a-淀粉酶的活性具有显著的抑制效应,50 mg.L-1及100 mg.L-1外源谷胱甘肽对这种抑制效应具有显著的缓解作用.