端到端网络环境下协同办公的应用分析

随着网络技术的发展,在下一代互联网技术和IPv6机制的支持下,网络的速度、质量、安全将得到加强,端到端技术将得到极大地应用。基于端到端技术的协同办公机制顺应扁平化发展趋势,使日常办公打破了时间和空间的限制,大大地提高了工作效率。     

网络经济下的电子商务成本分析

电子商务是21世纪新的经济增长点,代表着未来经济的发展方向,而目前电子商务的亏损使得人们对电子商务的成本进行分析和探讨。电子商务使交易形式由实体市场转为虚拟市场,这就可能导致交易成本的变化。B2B模式分析了电子商务的成本构成及电子商务下的企业交易成本的变化,探讨了B2B电子商务模式对交易成本的影响。     

不同二价阳离子对T-载体生成的影响

目的载体加dT尾是基于非模板扩增特性而发展起来的,是TA克隆技术的关键步骤之一,但实际应用中加尾率不高,为此对不同二价阳离子对载体加dT尾的影响效果进行比较,以摸索高效加尾条件。方法用限制性内切酶SmaI将质粒pGEM7Zf( )消化成平末端的线性片段,借助二价阳离子和Taq DNA聚合酶的作用在线性片段3-′末端上添加dTTP,随后与Control Insert DNA连接成环状DNA,再转化进E.coli DH5α-FT。根据白色菌落所占比例来评价不同种类和浓度的二价阳离子对T-载体生成的影响。结果不同种类和浓度的二价阳离子加尾效果比较显示,含1 mmol/L Cd2 缓冲体系的加尾效果最佳;Co2 及高浓度的Cd2 容易导致DNA反应体系变得浑浊,甚至出现白色沉淀析出的现象。结论Cd2 最有助于加尾,Mn2 次之,Mg2 最差。     

Replication,pathogenicity, and transmission of SARS-CoV-2 in minks

Minks are raised in many countries and have transmitted severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) to humans. However, the biologic properties of SARS-CoV-2 in minks are largely unknown. Here, we investigated and found that SARS-CoV-2 replicates efficiently in both the upper and lower respiratory tracts, and transmits efficiently in minks via respiratory droplets; pulmonary lesions caused by SARS-CoV-2 in minks are similar to those seen in humans with COVID-19. We further found that a spike protein-based subunit vaccine largely prevented SARS-CoV-2 replication and lung damage caused by SARS-CoV-2 infection in minks. Our study indicates that minks are a useful animal model for evaluating the efficacy of drugs or vaccines against COVID-19 and that vaccination is a potential strategy to prevent minks from transmitting SARS-CoV-2.     

优秀女子足球运动员间歇性恢复能力的评价研究

目的：：探讨运用限定级数YO-YO IR2测试以及心率恢复速率评价我国优秀女足运动员的间歇恢复能力。方法：运用测试法对2013年冬训期间的10名国家女足运动员进行YO-YO IR2测试、心率监控以及比赛监测,采集运动员测试跑动距离、心率特征以及比赛中高强度跑动距离,进行统计学分析。结论：女足运动员YO-YO IR2测试结果以及心率恢复速率与比赛中的高强度跑动能力具有紧密相关性,提示YO-YO IR2测试以及心率监控可以作为评价女足运动员间歇性恢复能力的有效方法。与传统的力竭性YO-YO IR2测试法相比,采用限定级数的YO-YO IR2测试后的心率恢复速率评价更易于女足运动员接受,在训练中具有更好的适用性。     

大负荷游泳运动后大鼠额叶神经元凋亡和线粒体形态变化及Drp1、Mfn2表达的研究

目的：：通过观察一次性大负荷游泳运动后线粒体形态结构以及线粒体融合分裂基因及蛋白表达的动态变化,探讨一次性大负荷游泳运动引起大鼠额叶神经元凋亡发生发展的可能机制。方法：72只雄性SD大鼠随机分为对照组(C组,n=18),一次性大负荷游泳运动组(E组,n=54),再根据运动后取材时间的不同将 E组分为：即刻组(E0组,n=18);24h组(E24组,n=18);48h组(E48组,n=18)。 C组常规饲养,E组进行一次性大负荷游泳运动,无负重游泳4小时。用透射电镜观察额叶神经元及神经元线粒体的形态结构;用TUNEL法检测细胞凋亡指数( AI);检测线粒体融合分裂基因Drp1、Mfn2 mRNA转录及蛋白表达水平。结果：检测发现E组大鼠大脑额叶神经元发生了细胞凋亡,E组各组大鼠AI显著性高于C组(P<0.01),E组各时间点AI比较为：E24>E0> E48,各组间差异均具有显著性( P<0.05)。一次性大负荷运动后各时间点Drp1和Mfn2 mRNA转录和蛋白表达均高于C组( P<0.05),其中E24组大鼠大脑额叶Drp1和Mfn2 mRNA转录和蛋白的表达最强(P<0.01);与E24组比较,E48组大鼠大脑额叶Drp1 mRNA转录和蛋白的表达下调显著(P<0.05),而Mfn2 mRNA转录和蛋白的表达下调不明显(P>0.05)。结论：一次性大负荷游泳运动引起大鼠额叶神经元凋亡及神经元线粒体形态结构异常,该异常在运动后48h内呈现动态性变化,机体可能通过调节线粒体融合分裂基因Drp1、Mfn2表达,影响大鼠额叶线粒体的形态结构和功能,从而调控额叶神经元凋亡的发生和发展。     

Kinship,Camaraderie and Contestation: Fāṭimid Relations with the Ashrāf in the Fourth/Tenth Century.

The founding of the Fā?imid caliphate across the southern Mediterranean, and then in Egypt, Syria and the ?ijāz at the turn of the fourth/tenth century, necessitated its negotiation with the ashrāf, those who claimed lineal descent from the Prophet Mu?ammad, and who by this time had gained significant influence as a social class based on their charismatic descent. While other dynastic powers fostered relationships with various members of the ashrāf, the Fā?imid–ashrāf dynamics were distinctive in that the Fā?imids legitimised their rule as Ismā?īlī Shī?ī imām-caliphs, based on their claim of descent from the Prophet Mu?ammad, and as the sole successors to his authority and leadership over the Islamic world. Consequently, Fā?imid–ashrāf relations were permeated by fraternal camaraderie as well as by competing contestations based on their shared claim to Prophetic lineage.     

The Impact of an Erasmus Placement in Students’ Perception of their Intercultural Communicative Competence

Pre- and post-questionnaires answered by UK Nottingham Trent University (NTU) and Spain’s University of Salamanca (USAL) students on their placement abroad support a comparative analysis of its impact on their intercultural communicative competence, comprising the awareness, attitude, skills and knowledge dimensions. NTU students started their placements with better results in them all. Yet, while both groups completed their stay with a similarly increased awareness, the knowledge and skills of the USAL group matched the results of NTU students and, whereas the scores of the attitude dimension towards the host country of NTU students did not increase significantly, those of USAL students decreased.     

非金融机构开展互联网金融的现况、影响及监管

2005年开始,国内兴起非金融机构经营互联网金融业务的热潮。非金融机构提供在线互联网金融服务,经营业务主要包括“支付”与“投、融资”两大类,其形态主要有四：第三方支付、基金销售、P2P网络借贷,以及众筹。非金融机构经营互联网金融,虽可加快国内金融业转型的步伐,并满足中小企业及个人无法自银行取得资金的投、融资需求,但也因发展过速,监管不足,致使网络借贷倒闭事件频传,不利于金融稳定等诸多负面效应。为此,需要主管机关研究制定与互联网金融相关的法律,加强监管,并准许这些非金融机构转型为正规商业银行。     

学术迹及其应用研究的综述

[目的/意义]综述目前学术迹在学术影响力评价中的应用研究。[方法/过程]从学术迹的概述、学术迹的相关指标、学术迹的应用与学术迹的缺陷及改进这4个方面系统地分析了学术迹作为新的指标体系在各学术主、客体学术影响力评价中的作用。[结果/结论]学术迹这一指标存在忽略论文合作现象、时效性差、评价不全面的缺陷,今后应加强和完善。